PMID- 30169651
OWN - NLM
STAT- MEDLINE
DCOM- 20190221
LR  - 20191204
IS  - 1525-3163 (Electronic)
IS  - 0021-8812 (Print)
IS  - 0021-8812 (Linking)
VI  - 96
IP  - 12
DP  - 2018 Dec 3
TI  - Dietary sulfur amino acids affect jejunal cell proliferation and functions by 
      affecting antioxidant capacity, Wnt/beta-catenin, and the mechanistic target of 
      rapamycin signaling pathways in weaning piglets.
PG  - 5124-5133
LID - 10.1093/jas/sky349 [doi]
AB  - Intestinal epithelial cells undergo rapid renewal along the crypt-villus axis 
      (CVA), which ensures intestinal functions. Weaning stress differentially effects 
      intestinal epithelial cell metabolism and physiological states along the CVA. 
      Sulfur amino acids (SAA) play a key role in intestinal epithelial cell 
      functioning. This study evaluated the effects of SAA dietary supplementation on 
      weaning pig jejunal epithelial cells along the CVA. Sixteen Duroc x Landrace x 
      Yorkshire piglets (6.16 +/- 0.22 kg BW) were weaned at 21 d of age and were blocked 
      by BW and gender and the randomly assigned to 1 of 2 groups fed diets consisting 
      of low (0.53%) or high (0.85%) levels of SAA for a 7-d period. All piglets were 
      euthanized for tissue sampling on day 7 postweaning. Jejunal epithelial cells 
      were isolated along the CVA to yield 3 "cell fractions" (upper villus, middle 
      villus, and crypt cells). The number of proliferating cells per crypt of piglets 
      fed the high SAA diet was lower (P < 0.05) than that for low SAA diet. High SAA 
      diet piglets tended to have decreased (P = 0.059) sucrase activities compared low 
      SAA diet piglets. A high SAA diet increased (P < 0.05) total antioxidant 
      capacity, catalase, and superoxide dismutase activities compared with a low SAA 
      diet. mRNA expression levels of claudin-1, Slc5a1, and Slc7a9 in high SAA diet 
      piglets were lower (P < 0.05) than for low SAA diet piglets. There were no 
      interactions between dietary SAA and cell sections along the CVA for enzyme 
      activities and mRNA expression in any of the weaned piglets. Protein amounts and 
      phosphorylation levels related to Wnt/beta-catenin and mechanistic targeting of 
      rapamycin (mTOR) signaling pathways were affected by SAA in weaning piglets. 
      These findings indicate that dietary SAA affects jejunal cell proliferation and 
      functions in weaning piglets. There appears to be no interactions between dietary 
      SAA and cell sections along the CVA. The effects of SAA may be partly through 
      affecting antioxidant capacity, and Wnt/beta-catenin and mTOR signaling pathway.
FAU - Yan, Shanling
AU  - Yan S
AD  - Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, 
      Animal Nutrition and Human Health Laboratory, School of Life Sciences, Hunan 
      Normal University, Changsha City, Hunan, China.
FAU - Long, Lina
AU  - Long L
AD  - Chinese Academy of Science, Institute of Subtropical Agriculture, Research Center 
      for Healthy Breeding of Livestock and Poultry, Hunan Engineering and Research 
      Center of Animal and Poultry Science and Key Laboratory for Agroecological 
      Processes in Subtropical Region, Scientific Observation and Experimental Station 
      of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, 
      Changsha City, Hunan, China.
FAU - Zong, Enyan
AU  - Zong E
AD  - Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, 
      Animal Nutrition and Human Health Laboratory, School of Life Sciences, Hunan 
      Normal University, Changsha City, Hunan, China.
FAU - Huang, Pengfei
AU  - Huang P
AD  - Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, 
      Animal Nutrition and Human Health Laboratory, School of Life Sciences, Hunan 
      Normal University, Changsha City, Hunan, China.
FAU - Li, Jianzhong
AU  - Li J
AD  - Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, 
      Animal Nutrition and Human Health Laboratory, School of Life Sciences, Hunan 
      Normal University, Changsha City, Hunan, China.
FAU - Li, Yali
AU  - Li Y
AD  - Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, 
      Animal Nutrition and Human Health Laboratory, School of Life Sciences, Hunan 
      Normal University, Changsha City, Hunan, China.
FAU - Ding, Xueqin
AU  - Ding X
AD  - Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, 
      Animal Nutrition and Human Health Laboratory, School of Life Sciences, Hunan 
      Normal University, Changsha City, Hunan, China.
FAU - Xiong, Xia
AU  - Xiong X
AD  - Chinese Academy of Science, Institute of Subtropical Agriculture, Research Center 
      for Healthy Breeding of Livestock and Poultry, Hunan Engineering and Research 
      Center of Animal and Poultry Science and Key Laboratory for Agroecological 
      Processes in Subtropical Region, Scientific Observation and Experimental Station 
      of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, 
      Changsha City, Hunan, China.
FAU - Yin, Yulong
AU  - Yin Y
AD  - Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, 
      Animal Nutrition and Human Health Laboratory, School of Life Sciences, Hunan 
      Normal University, Changsha City, Hunan, China.
AD  - Chinese Academy of Science, Institute of Subtropical Agriculture, Research Center 
      for Healthy Breeding of Livestock and Poultry, Hunan Engineering and Research 
      Center of Animal and Poultry Science and Key Laboratory for Agroecological 
      Processes in Subtropical Region, Scientific Observation and Experimental Station 
      of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, 
      Changsha City, Hunan, China.
FAU - Yang, Huansheng
AU  - Yang H
AD  - Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, 
      Animal Nutrition and Human Health Laboratory, School of Life Sciences, Hunan 
      Normal University, Changsha City, Hunan, China.
AD  - Chinese Academy of Science, Institute of Subtropical Agriculture, Research Center 
      for Healthy Breeding of Livestock and Poultry, Hunan Engineering and Research 
      Center of Animal and Poultry Science and Key Laboratory for Agroecological 
      Processes in Subtropical Region, Scientific Observation and Experimental Station 
      of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, 
      Changsha City, Hunan, China.
LA  - eng
PT  - Clinical Trial, Veterinary
PT  - Journal Article
PL  - United States
TA  - J Anim Sci
JT  - Journal of animal science
JID - 8003002
RN  - 0 (Amino Acids, Sulfur)
RN  - 0 (Antioxidants)
RN  - 0 (beta Catenin)
SB  - IM
MH  - Amino Acids, Sulfur/administration & dosage/*pharmacology
MH  - Animal Feed/*analysis
MH  - Animal Nutritional Physiological Phenomena
MH  - Animals
MH  - Antioxidants/metabolism
MH  - Cell Proliferation
MH  - Diet/*veterinary
MH  - Dietary Supplements
MH  - Epithelial Cells/drug effects/metabolism
MH  - Intestinal Mucosa/metabolism
MH  - Jejunum/cytology/*drug effects/metabolism
MH  - Random Allocation
MH  - Signal Transduction
MH  - Swine/*physiology
MH  - beta Catenin/*metabolism
PMC - PMC6276574
EDAT- 2018/09/01 06:00
MHDA- 2019/02/23 06:00
PMCR- 2019/12/03
CRDT- 2018/09/01 06:00
PHST- 2018/04/11 00:00 [received]
PHST- 2018/08/25 00:00 [accepted]
PHST- 2018/09/01 06:00 [pubmed]
PHST- 2019/02/23 06:00 [medline]
PHST- 2018/09/01 06:00 [entrez]
PHST- 2019/12/03 00:00 [pmc-release]
AID - 5085566 [pii]
AID - sky349 [pii]
AID - 10.1093/jas/sky349 [doi]
PST - ppublish
SO  - J Anim Sci. 2018 Dec 3;96(12):5124-5133. doi: 10.1093/jas/sky349.