PMID- 30170587 OWN - NLM STAT- MEDLINE DCOM- 20181108 LR - 20181114 IS - 1746-6148 (Electronic) IS - 1746-6148 (Linking) VI - 14 IP - 1 DP - 2018 Aug 31 TI - Visual and equipment-free reverse transcription recombinase polymerase amplification method for rapid detection of foot-and-mouth disease virus. PG - 263 LID - 10.1186/s12917-018-1594-x [doi] LID - 263 AB - BACKGROUND: Foot-and-mouth disease (FMD), which is caused by foot-and-mouth disease virus (FMDV), is a highly contagious tansboundary disease of cloven-hoofed animals and causes devastating economic damages. Accurate, rapid and simple detection of FMDV is critical to containing an FMD outbreak. Recombinase polymerase amplification (RPA) has been explored for detection of diverse pathogens because of its accuracy, rapidness and simplicity. A visible and equipment-free reverse-transcription recombinase polymerase amplification assay combined with lateral flow strip (LFS RT-RPA) was developed to detect the FMDV using primers and LF probe specific for the 3D gene. RESULTS: The FMDV LFS RT-RPA assay was performed successfully in a closed fist using body heat for 15 min, and the products were visible on the LFS inspected by the naked eyes within 2 min. The assay could detect FMDV serotypes O, A and Asia1, and there were no cross-reactions with vesicular stomatitis virus (VSV), encephalomyocarditis virus (EMCV), classical swine fever virus (CSFV), porcine reproductive and respiratory syndrome virus (PRRSV), porcine circovirus 2 (PCV2) and pseudorabies virus (PRV). The analytical sensitivity was 1.0 x 10(2) copies in vitro transcribed FMDV RNA per reaction, which was the same as a real-time RT-PCR. For the 55 samples, FMDV RNA positive rate was 45.5% (25/55) by LFS RT-RPA and 52.7% (29/55) by real-time RT-PCR. For the LFS RT-RPA assay, the positive and negative predicative values were 100% and 80%, respectively. CONCLUSIONS: The performance of the LFS RT-RPA assay was comparable to real-time RT-PCR, while the LFS RT-RPA assay was much faster and easier to be performed. The developed FMDV LFS RT-RPA assay provides an attractive and promising tool for rapid and reliable detection of FMDV in under-equipped laboratory and at point-of-need facility, which is of great significance in FMD control in low resource settings. FAU - Liu, Libing AU - Liu L AD - Center of Inspection and Quarantine, Hebei Entry-Exit Inspection and Quarantine Bureau, Shijiazhuang, 050051, People's Republic of China. FAU - Wang, Jinfeng AU - Wang J AD - Center of Inspection and Quarantine, Hebei Entry-Exit Inspection and Quarantine Bureau, Shijiazhuang, 050051, People's Republic of China. FAU - Zhang, Ruoxi AU - Zhang R AD - Hebei Animal Disease Control Center, Shijiazhuang, 050050, People's Republic of China. FAU - Lin, Mi AU - Lin M AD - State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, People's Republic of China. FAU - Shi, Ruihan AU - Shi R AD - Center of Inspection and Quarantine, Hebei Entry-Exit Inspection and Quarantine Bureau, Shijiazhuang, 050051, People's Republic of China. AD - Hebei Academy of Science and Technology for Inspection and Quarantine, Shijiazhuang, 050051, People's Republic of China. FAU - Han, Qingan AU - Han Q AD - Hebei Animal Disease Control Center, Shijiazhuang, 050050, People's Republic of China. FAU - Wang, Jianchang AU - Wang J AD - Center of Inspection and Quarantine, Hebei Entry-Exit Inspection and Quarantine Bureau, Shijiazhuang, 050051, People's Republic of China. jianchangwang1225@126.com. AD - Hebei Academy of Science and Technology for Inspection and Quarantine, Shijiazhuang, 050051, People's Republic of China. jianchangwang1225@126.com. FAU - Yuan, Wanzhe AU - Yuan W AUID- ORCID: 0000-0003-2442-0539 AD - College of Veterinary Medicine, Agricultural University of Hebei, No.38 Lingyusi Street, Baoding, Hebei, 071001, People's Republic of China. yuanwanzhe2015@126.com. LA - eng GR - C2017325001/Natural Science Foundation Youth Project of Hebei Province/ GR - 16226604D/Science and Technology Project Foundation of Hebei Province/ GR - SLRC2017039/fund for one-hundred outstanding innovative talents from Hebei institution of higher learning/ GR - HBCT2018140204/Earmarked Fund for Hebei Sheep&Goat Innovation team of Modern Agro-industry technology Research System/ PT - Journal Article DEP - 20180831 PL - England TA - BMC Vet Res JT - BMC veterinary research JID - 101249759 RN - 0 (Reagent Strips) RN - 0 (Recombinases) SB - IM MH - Animals MH - Cattle MH - Foot-and-Mouth Disease/blood/*diagnosis MH - Foot-and-Mouth Disease Virus/*genetics/*isolation & purification MH - Nucleic Acid Amplification Techniques/methods/*veterinary MH - Reagent Strips MH - Recombinases MH - *Reverse Transcription MH - Sensitivity and Specificity MH - Serogroup MH - Swine PMC - PMC6119248 OTO - NOTNLM OT - 3D gene OT - FMDV OT - LF probe OT - Lateral flow strip OT - RPA COIS- ETHICS APPROVAL AND CONSENT TO PARTICIPATE: This study was approved by the Institutional Animal Care and Ethics Committee of Agricultural University of Hebei (approval no. IACECHEBAU20110509). CONSENT FOR PUBLICATION: Not applicable. COMPETING INTERESTS: The authors declare that they have no competing interests. PUBLISHER'S NOTE: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. EDAT- 2018/09/02 06:00 MHDA- 2018/11/09 06:00 PMCR- 2018/08/31 CRDT- 2018/09/02 06:00 PHST- 2018/01/23 00:00 [received] PHST- 2018/08/24 00:00 [accepted] PHST- 2018/09/02 06:00 [entrez] PHST- 2018/09/02 06:00 [pubmed] PHST- 2018/11/09 06:00 [medline] PHST- 2018/08/31 00:00 [pmc-release] AID - 10.1186/s12917-018-1594-x [pii] AID - 1594 [pii] AID - 10.1186/s12917-018-1594-x [doi] PST - epublish SO - BMC Vet Res. 2018 Aug 31;14(1):263. doi: 10.1186/s12917-018-1594-x.