PMID- 30172411
OWN - NLM
STAT- MEDLINE
DCOM- 20181231
LR  - 20181231
IS  - 1525-3198 (Electronic)
IS  - 0022-0302 (Linking)
VI  - 101
IP  - 11
DP  - 2018 Nov
TI  - Variation in fibroblast expression of toll-like receptor 4 and 
      lipopolysaccharide-induced cytokine production between animals predicts control 
      of bacterial growth but not severity of Escherichia coli mastitis.
PG  - 10098-10115
LID - S0022-0302(18)30784-7 [pii]
LID - 10.3168/jds.2017-14372 [doi]
AB  - Mastitis caused by environmental pathogens such as Escherichia coli is highly 
      problematic to the dairy industry because it incurs substantial cost and tends to 
      be difficult to manage. An effective innate immune response by the host is key to 
      controlling infection, but it should also limit collateral damage to the mammary 
      gland. Between-animal differences in mastitis severity have been attributed to 
      variability in the innate response. In the current study, we used primary dermal 
      fibroblast as a model to rank animals based on composite expression of the 
      toll-like receptor 4 gene (TLR4) and lipopolysaccharide (LPS)-induced IL-8 and 
      IL-6 protein production. Animals ranked as high and low responders (HR and LR, 
      respectively) were then infected with the P4 strain of E. coli to determine how 
      difference in rank would affect response to mastitis. All animals developed an 
      acute response to the infection with varying degrees in severity; however, HR 
      animals had an elevated somatic cell count and fever response at 12 h 
      post-infection and greater production of milk IL-8 at 24 h post-infection. The HR 
      animals were also significantly more capable of limiting bacterial growth. No 
      differences in post-infection milk production or concentrations of milk BSA were 
      measured. The current study indicates that HR animals have an early upregulation 
      in their innate response that is beneficial for bacterial clearance; however, 
      they are equally susceptible to tissue damage caused by an exuberant response to 
      the infection. The dermal fibroblast may be used in conjunction with other cell 
      types to determine how the innate response is regulated to mitigate unnecessary 
      injury to the mammary gland while still effectively clearing the pathogen.
CI  - Copyright (c) 2018 American Dairy Science Association. Published by Elsevier Inc. 
      All rights reserved.
FAU - Korkmaz, F T
AU  - Korkmaz FT
AD  - Department of Animal and Veterinary Sciences, College of Agriculture and Life 
      Sciences, Burlington 05405; Cellular, Molecular and Biomedical Sciences Program, 
      University of Vermont, Burlington 05405.
FAU - Elsasser, T H
AU  - Elsasser TH
AD  - Animal Genomics and Improvement Laboratory, USDA, Agricultural Research Service, 
      Beltsville, MD 20705.
FAU - Kerr, D E
AU  - Kerr DE
AD  - Department of Animal and Veterinary Sciences, College of Agriculture and Life 
      Sciences, Burlington 05405; Cellular, Molecular and Biomedical Sciences Program, 
      University of Vermont, Burlington 05405. Electronic address: david.kerr@uvm.edu.
LA  - eng
PT  - Journal Article
DEP - 20180829
PL  - United States
TA  - J Dairy Sci
JT  - Journal of dairy science
JID - 2985126R
RN  - 0 (Interleukin-6)
RN  - 0 (Interleukin-8)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Toll-Like Receptor 4)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Cell Count/veterinary
MH  - Dairying
MH  - Escherichia coli/*immunology
MH  - Escherichia coli Infections/*immunology/microbiology
MH  - Female
MH  - Fibroblasts/drug effects/immunology
MH  - Gene Expression Regulation
MH  - *Immunity, Innate
MH  - Interleukin-6/immunology
MH  - Interleukin-8/immunology
MH  - Lipopolysaccharides/*immunology/pharmacology
MH  - Mastitis, Bovine/*immunology/microbiology
MH  - Milk/metabolism
MH  - Toll-Like Receptor 4/*immunology
OTO - NOTNLM
OT  - dairy cow
OT  - experimental mastitis
OT  - innate immunity
OT  - lipopolysaccharide
EDAT- 2018/09/03 06:00
MHDA- 2019/01/01 06:00
CRDT- 2018/09/03 06:00
PHST- 2017/12/31 00:00 [received]
PHST- 2018/06/12 00:00 [accepted]
PHST- 2018/09/03 06:00 [pubmed]
PHST- 2019/01/01 06:00 [medline]
PHST- 2018/09/03 06:00 [entrez]
AID - S0022-0302(18)30784-7 [pii]
AID - 10.3168/jds.2017-14372 [doi]
PST - ppublish
SO  - J Dairy Sci. 2018 Nov;101(11):10098-10115. doi: 10.3168/jds.2017-14372. Epub 2018 
      Aug 29.