PMID- 30172909
OWN - NLM
STAT- MEDLINE
DCOM- 20190408
LR  - 20200225
IS  - 1879-0089 (Electronic)
IS  - 0145-305X (Print)
IS  - 0145-305X (Linking)
VI  - 90
DP  - 2019 Jan
TI  - Innate immune cell signatures in a BCWD-Resistant line of rainbow trout before 
      and after in vivo challenge with Flavobacterium psychrophilum.
PG  - 47-54
LID - S0145-305X(18)30274-X [pii]
LID - 10.1016/j.dci.2018.08.018 [doi]
AB  - Phenotypes of myeloid-lineage cells remain poorly understood in the rainbow 
      trout, and were the focus of this study, including effects of in vivo challenge 
      to Flavobacterium psychrophilum (Fp), the cause of Bacterial Cold Water Disease 
      (BCWD). A genetic line was used that is highly resistant to BCWD (R-line) as well 
      as a susceptible control line (S-line). Using flow cytometry, we describe two 
      Pax5-negative, myeloid-lineage populations: Population 1 consisted of small cells 
      with high SSC and strong staining for Q4E, MPO, Pu1, EBF, and IL- 1beta, which we 
      named "neutrophil-like" cell. Population 2 had high Q4E, but weaker MPO, Pu1, 
      EBF, and IL-1beta staining. Five days after Fp-challenge, both genetic lines had a 
      reduced abundance of neutrophil-like cells in anterior kidney, PBL, and spleen. 
      Pop. 2 abundance was reduced in anterior kidney, and increased in spleen. S-line 
      fish responded more strongly to Fp-challenge compared to R-line fish. Challenged 
      fish with a higher abundance of neutrophil-like cells had significantly lower 
      Fp-loads after challenge, suggesting that these cells aid in the resistance to 
      BCWD.
CI  - Copyright (c) 2018 Elsevier Ltd. All rights reserved.
FAU - Moore, Catherine
AU  - Moore C
AD  - Department of Biology, The College of William and Mary, Williamsburg, VA, 23185, 
      USA.
FAU - Hennessey, Erin
AU  - Hennessey E
AD  - Department of Biology, The College of William and Mary, Williamsburg, VA, 23185, 
      USA.
FAU - Smith, Meaghan
AU  - Smith M
AD  - Department of Biology, The College of William and Mary, Williamsburg, VA, 23185, 
      USA.
FAU - Epp, Lidia
AU  - Epp L
AD  - Department of Biology, The College of William and Mary, Williamsburg, VA, 23185, 
      USA.
FAU - Zwollo, Patty
AU  - Zwollo P
AD  - Department of Biology, The College of William and Mary, Williamsburg, VA, 23185, 
      USA. Electronic address: pxzwol@wm.edu.
LA  - eng
GR  - R15 AI119978/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20180830
PL  - United States
TA  - Dev Comp Immunol
JT  - Developmental and comparative immunology
JID - 7708205
RN  - 0 (PAX5 Transcription Factor)
SB  - IM
MH  - Animals
MH  - Bacterial Load
MH  - Cell Line
MH  - Disease Resistance
MH  - Fish Diseases/*immunology
MH  - Flavobacteriaceae Infections/*immunology
MH  - Flavobacterium/*physiology
MH  - Genetic Predisposition to Disease
MH  - Immunity, Innate
MH  - Myeloid Cells/*physiology
MH  - Neutrophils/*immunology
MH  - Oncorhynchus mykiss/genetics/*immunology
MH  - PAX5 Transcription Factor/*genetics
MH  - Transcriptome
PMC - PMC6436949
MID - NIHMS1506289
EDAT- 2018/09/03 06:00
MHDA- 2019/04/09 06:00
PMCR- 2020/01/01
CRDT- 2018/09/03 06:00
PHST- 2018/05/25 00:00 [received]
PHST- 2018/08/27 00:00 [revised]
PHST- 2018/08/27 00:00 [accepted]
PHST- 2018/09/03 06:00 [pubmed]
PHST- 2019/04/09 06:00 [medline]
PHST- 2018/09/03 06:00 [entrez]
PHST- 2020/01/01 00:00 [pmc-release]
AID - S0145-305X(18)30274-X [pii]
AID - 10.1016/j.dci.2018.08.018 [doi]
PST - ppublish
SO  - Dev Comp Immunol. 2019 Jan;90:47-54. doi: 10.1016/j.dci.2018.08.018. Epub 2018 
      Aug 30.