PMID- 30174716
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1741-427X (Print)
IS  - 1741-4288 (Electronic)
IS  - 1741-427X (Linking)
VI  - 2018
DP  - 2018
TI  - Aqueous Extract of Glucoraphanin-Rich Broccoli Sprouts Inhibits Formation of 
      Advanced Glycation End Products and Attenuates Inflammatory Reactions in 
      Endothelial Cells.
PG  - 9823141
LID - 10.1155/2018/9823141 [doi]
LID - 9823141
AB  - We have previously shown that sulforaphane not only inhibits formation of 
      advanced glycation end products (AGEs) but also exerts anti-inflammatory effects 
      on AGE-exposed human umbilical vein endothelial cells (HUVECs) and AGE-injected 
      rat aortae. Here we examined the effects of aqueous extract of glucoraphanin-rich 
      broccoli sprouts on formation of AGEs and then investigated whether the extract 
      could attenuate inflammatory or oxidative stress reactions in tumor necrosis 
      factor-alpha (TNF-alpha)- or AGE-exposed HUVECs. Fresh broccoli sprouts were 
      homogenized in phosphate-buffered saline and filtered through a gauze. After 
      centrifugation, clear extract was obtained. AGE formation was measured by 
      enzyme-linked immunosorbent assay. Gene expression was evaluated by real-time 
      reverse transcription-polymerase chain reaction. Reactive oxygen species (ROS) 
      generation were measured using a fluorescent dye. Five percent broccoli sprout 
      extract inhibited the formation of AGEs, reduced basal gene expressions of 
      monocyte chemoattractant protein-1 (MCP-1), intercellular adhesion molecule-1 
      (ICAM-1,) and receptor for AGEs (RAGE), and upregulated endothelial nitric oxide 
      synthase (eNOS) mRNA levels in HUVECs. TNF-alpha upregulated MCP-1, ICAM-1, and RAGE 
      mRNA levels in HUVECs, all of which were attenuated by the treatment with 1% 
      broccoli sprout extract. Pretreatment of 1% broccoli sprout extract prevented the 
      ROS generation in HUVECs evoked by AGEs. The present study demonstrates that 
      sulforaphane-rich broccoli sprout extract could inhibit the AGE-RAGE axis and 
      exhibit anti-inflammatory actions in HUVECs. Supplementation of sulforaphane-rich 
      broccoli sprout extract may play a protective role against vascular injury.
FAU - Sotokawauchi, Ami
AU  - Sotokawauchi A
AD  - Department of Pathophysiology and Therapeutics of Diabetic Vascular 
      Complications, Kurume University School of Medicine, Kurume, Japan.
FAU - Ishibashi, Yuji
AU  - Ishibashi Y
AD  - Department of Pathophysiology and Therapeutics of Diabetic Vascular 
      Complications, Kurume University School of Medicine, Kurume, Japan.
FAU - Matsui, Takanori
AU  - Matsui T
AD  - Department of Pathophysiology and Therapeutics of Diabetic Vascular 
      Complications, Kurume University School of Medicine, Kurume, Japan.
FAU - Yamagishi, Sho-Ichi
AU  - Yamagishi SI
AUID- ORCID: 0000-0003-0102-0823
AD  - Department of Pathophysiology and Therapeutics of Diabetic Vascular 
      Complications, Kurume University School of Medicine, Kurume, Japan.
LA  - eng
PT  - Journal Article
DEP - 20180808
PL  - United States
TA  - Evid Based Complement Alternat Med
JT  - Evidence-based complementary and alternative medicine : eCAM
JID - 101215021
PMC - PMC6106845
EDAT- 2018/09/04 06:00
MHDA- 2018/09/04 06:01
PMCR- 2018/08/08
CRDT- 2018/09/04 06:00
PHST- 2018/06/21 00:00 [received]
PHST- 2018/07/27 00:00 [revised]
PHST- 2018/08/01 00:00 [accepted]
PHST- 2018/09/04 06:00 [entrez]
PHST- 2018/09/04 06:00 [pubmed]
PHST- 2018/09/04 06:01 [medline]
PHST- 2018/08/08 00:00 [pmc-release]
AID - 10.1155/2018/9823141 [doi]
PST - epublish
SO  - Evid Based Complement Alternat Med. 2018 Aug 8;2018:9823141. doi: 
      10.1155/2018/9823141. eCollection 2018.