PMID- 30176067
OWN - NLM
STAT- MEDLINE
DCOM- 20200422
LR  - 20200422
IS  - 1098-2396 (Electronic)
IS  - 0887-4476 (Linking)
VI  - 73
IP  - 1
DP  - 2019 Jan
TI  - Quantitative proteomics of forebrain subcellular fractions in fragile X mental 
      retardation 1 knockout mice following acute treatment with 
      2-Methyl-6-(phenylethynyl)pyridine: Relevance to developmental study of 
      schizophrenia.
PG  - e22069
LID - 10.1002/syn.22069 [doi]
AB  - The fragile X mental retardation 1 knockout (Fmr1 KO) mouse replicates behavioral 
      deficits associated with autism, fragile X syndrome, and schizophrenia. Less is 
      known whether protein expression changes are consistent with findings in subjects 
      with schizophrenia. In the current study, we used liquid chromatography tandem 
      mass spectrometry (LC-MS/MS) proteomics to determine the protein expression of 
      four subcellular fractions in the forebrains of Fmr1 KO mice vs. C57BL/6 J mice 
      and the effect of a negative allosteric modulator of 
      mGluR5-2-Methyl-6-(phenylethynyl)pyridine (MPEP)-on protein expression. Strain- 
      and treatment-specific differential expression of proteins was observed, many of 
      which have previously been observed in the brains of subjects with schizophrenia. 
      Western blotting verified the direction and magnitude of change for several 
      proteins in different subcellular fractions as follows: neurofilament light 
      protein (NEFL) and 2',3'-cyclic-nucleotide 3'-phosphodiesterase (CNP) in the 
      total homogenate; heterogeneous nuclear ribonucleoproteins C1/C2 (HNRNPC) and 
      heterogeneous nuclear ribonucleoprotein D0 (HNRNPD) in the nuclear fraction; 
      excitatory amino acid transporter 2 (EAAT2) and ras-related protein rab 3a 
      (RAB3A) in the synaptic fraction; and ras-related protein rab 35 (RAB35) and 
      neuromodulin (GAP43) in the rough endoplasmic reticulum fraction. Individuals 
      with FXS do not display symptoms of schizophrenia. However, the biomarkers that 
      have been identified suggest that the Fmr1 KO model could potentially be useful 
      in the study of schizophrenia.
CI  - (c) 2018 Wiley Periodicals, Inc.
FAU - Folsom, Timothy D
AU  - Folsom TD
AD  - Department of Psychiatry, Division of Neuroscience Research, University of 
      Minnesota Medical School, Minneapolis, Minnesota.
FAU - Higgins, LeeAnn
AU  - Higgins L
AD  - Department of Biochemistry, Molecular Biology, and Biophysics, University of 
      Minnesota Medical School, Minneapolis, Minnesota.
FAU - Markowski, Todd W
AU  - Markowski TW
AD  - Department of Biochemistry, Molecular Biology, and Biophysics, University of 
      Minnesota Medical School, Minneapolis, Minnesota.
FAU - Griffin, Timothy J
AU  - Griffin TJ
AD  - Department of Biochemistry, Molecular Biology, and Biophysics, University of 
      Minnesota Medical School, Minneapolis, Minnesota.
FAU - Fatemi, S Hossein
AU  - Fatemi SH
AUID- ORCID: 0000-0002-6859-2626
AD  - Department of Psychiatry, Division of Neuroscience Research, University of 
      Minnesota Medical School, Minneapolis, Minnesota.
AD  - Department of Neuroscience, University of Minnesota Medical School, Minneapolis, 
      Minnesota.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20181003
PL  - United States
TA  - Synapse
JT  - Synapse (New York, N.Y.)
JID - 8806914
RN  - 0 (Excitatory Amino Acid Antagonists)
RN  - 0 (Excitatory Amino Acid Transporter 2)
RN  - 0 (Fmr1 protein, mouse)
RN  - 0 (GAP-43 Protein)
RN  - 0 (Neurofilament Proteins)
RN  - 0 (Proteome)
RN  - 0 (Pyridines)
RN  - 0 (Ribonucleoproteins)
RN  - 0 (Slc1a2 protein, mouse)
RN  - 0 (neurofilament protein L)
RN  - 139135-51-6 (Fragile X Mental Retardation Protein)
RN  - 7VC0YVI27Y (6-methyl-2-(phenylethynyl)pyridine)
RN  - EC 3.1.4.37 (2',3'-Cyclic Nucleotide 3'-Phosphodiesterase)
RN  - EC 3.1.4.37 (Cnp protein, mouse)
RN  - EC 3.6.5.2 (rab GTP-Binding Proteins)
SB  - IM
MH  - 2',3'-Cyclic Nucleotide 3'-Phosphodiesterase/genetics/metabolism
MH  - Animals
MH  - Excitatory Amino Acid Antagonists/*pharmacology
MH  - Excitatory Amino Acid Transporter 2/genetics/metabolism
MH  - Fragile X Mental Retardation Protein/*genetics
MH  - GAP-43 Protein/genetics/metabolism
MH  - Gene Deletion
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Neurofilament Proteins/genetics/metabolism
MH  - Prosencephalon/drug effects/*metabolism
MH  - Proteome/*genetics/metabolism
MH  - Pyridines/*pharmacology
MH  - Ribonucleoproteins/genetics/metabolism
MH  - Schizophrenia/*genetics/metabolism
MH  - rab GTP-Binding Proteins/genetics/metabolism
OTO - NOTNLM
OT  - FMRP
OT  - FXS
OT  - MPEP
OT  - autism
OT  - brain
OT  - mGluR5
OT  - schizophrenia
EDAT- 2018/09/04 06:00
MHDA- 2020/04/23 06:00
CRDT- 2018/09/04 06:00
PHST- 2018/03/15 00:00 [received]
PHST- 2018/08/13 00:00 [revised]
PHST- 2018/08/30 00:00 [accepted]
PHST- 2018/09/04 06:00 [pubmed]
PHST- 2020/04/23 06:00 [medline]
PHST- 2018/09/04 06:00 [entrez]
AID - 10.1002/syn.22069 [doi]
PST - ppublish
SO  - Synapse. 2019 Jan;73(1):e22069. doi: 10.1002/syn.22069. Epub 2018 Oct 3.