PMID- 30177602
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 2305-6304 (Electronic)
IS  - 2305-6304 (Linking)
VI  - 6
IP  - 3
DP  - 2018 Sep 3
TI  - Intrauterine Exposure to Cadmium Reduces HIF-1 DNA-Binding Ability in Rat Fetal 
      Kidneys.
LID - 10.3390/toxics6030053 [doi]
LID - 53
AB  - During embryonic development, some hypoxia occurs due to incipient 
      vascularization. Under hypoxic conditions, gene expression is mainly controlled 
      by hypoxia-inducible factor 1 (HIF-1). The activity of this transcription factor 
      can be altered by the exposure to a variety of compounds; among them is cadmium 
      (Cd), a nephrotoxic heavy metal capable of crossing the placenta and reaching 
      fetal kidneys. The goal of the study was to determine Cd effects on HIF-1 on 
      embryonic kidneys. Pregnant Wistar rats were exposed to a mist of isotonic saline 
      solution or CdCl(2) (D(Del) = 1.48 mg Cd/kg/day), from gestational day (GD) 8 to 
      20. Embryonic kidneys were obtained on GD 21 for RNA and protein extraction. 
      Results show that Cd exposure had no effect on HIF-1alpha and prolyl hydroxylase 2 
      protein levels, but it reduced HIF-1 DNA-binding ability, which was confirmed by 
      a decrease in vascular endothelial growth factor (VEGF) mRNA levels. In contrast, 
      the protein levels of VEGF were not changed, which suggests the activation of 
      additional regulatory mechanisms of VEGF protein expression to ensure proper 
      kidney development. In conclusion, Cd exposure decreases HIF-1-binding activity, 
      posing a risk on renal fetal development.
FAU - Jacobo-Estrada, Tania
AU  - Jacobo-Estrada T
AUID- ORCID: 0000-0001-9682-8627
AD  - Departamento de Toxicologia, Centro de Investigacion y de Estudios Avanzados del 
      Instituto Politecnico Nacional, 07360 Ciudad de Mexico, Mexico. tjacoboe@ipn.mx.
AD  - Departamento de Sociedad y Politica Ambiental, CIIEMAD, Instituto Politecnico 
      Nacional, 07340 Ciudad de Mexico, Mexico. tjacoboe@ipn.mx.
FAU - Cardenas-Gonzalez, Mariana
AU  - Cardenas-Gonzalez M
AUID- ORCID: 0000-0003-4154-6088
AD  - Renal Division, Department of Medicine, Brigham and Women's Hospital, Harvard 
      Medical School, Boston, MA 02115, USA. mcardenasgonzalez@bwh.harvard.edu.
FAU - Santoyo-Sanchez, Mitzi Paola
AU  - Santoyo-Sanchez MP
AD  - Departamento de Toxicologia, Centro de Investigacion y de Estudios Avanzados del 
      Instituto Politecnico Nacional, 07360 Ciudad de Mexico, Mexico. 
      santoyomitzi@gmail.com.
FAU - Thevenod, Frank
AU  - Thevenod F
AD  - Department of Physiology, Pathophysiology and Toxicology and ZBAF (Center for 
      Biomedical Education and Research), Faculty of Health-School of Medicine, 
      Witten/Herdecke University, 58448 Witten, Germany. frank.thevenod@uni-wh.de.
FAU - Barbier, Olivier
AU  - Barbier O
AUID- ORCID: 0000-0002-0378-3675
AD  - Departamento de Toxicologia, Centro de Investigacion y de Estudios Avanzados del 
      Instituto Politecnico Nacional, 07360 Ciudad de Mexico, Mexico. 
      obarbier@cinvestav.mx.
LA  - eng
GR  - PICSA12-086/Secretaria de Ciencia, Tecnologia e Innovacion del Distrito Federal/
GR  - 267755/Consejo Nacional de Ciencia y Tecnologia CONACYT/FONCICYT/
GR  - 326697/Consejo Nacional de Ciencia y Tecnologia/
PT  - Journal Article
DEP - 20180903
PL  - Switzerland
TA  - Toxics
JT  - Toxics
JID - 101639637
PMC - PMC6161213
OTO - NOTNLM
OT  - HIF-1
OT  - cadmium
OT  - embryonic kidneys
OT  - intrauterine exposure
COIS- The authors declare no conflict of interest. The funding sponsors had no role in 
      the design of the study; in the collection, analyses, or interpretation of data; 
      in the writing of the manuscript; and in the decision to publish the results.
EDAT- 2018/09/05 06:00
MHDA- 2018/09/05 06:01
PMCR- 2018/09/03
CRDT- 2018/09/05 06:00
PHST- 2018/06/11 00:00 [received]
PHST- 2018/08/07 00:00 [revised]
PHST- 2018/08/29 00:00 [accepted]
PHST- 2018/09/05 06:00 [entrez]
PHST- 2018/09/05 06:00 [pubmed]
PHST- 2018/09/05 06:01 [medline]
PHST- 2018/09/03 00:00 [pmc-release]
AID - toxics6030053 [pii]
AID - toxics-06-00053 [pii]
AID - 10.3390/toxics6030053 [doi]
PST - epublish
SO  - Toxics. 2018 Sep 3;6(3):53. doi: 10.3390/toxics6030053.