PMID- 3018079
OWN - NLM
STAT- MEDLINE
DCOM- 19860926
LR  - 20220310
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 137
IP  - 5
DP  - 1986 Sep 1
TI  - Involvement of natural killer cells in coxsackievirus B3-induced murine 
      myocarditis.
PG  - 1695-702
AB  - The role of natural killer cells in the temporal development of coxsackievirus 
      B3-induced myocarditis in adolescent CD-1 male mice was examined. Inoculation of 
      purified CVB3m induced maximum NK cell activity in the splenic populations at 3 
      days postinoculation (p.i.) as assessed by lysis of YAC-1 cells; maximum virus 
      titers in heart tissues were also found at day 3 p.i. Mice depleted of NK cells 
      after injection of anti-asialo GM1 antiserum i.v. had decreased NK cell activity, 
      increased CVB3m titers in heart tissues, and exacerbated myocarditis. Although 
      lesion number was not increased in heart tissues of the latter mice, lesions in 
      these mice exhibited increased myocyte degeneration and dystrophic calcification 
      above that found in lesions of mice inoculated with CVB3m only. No alteration in 
      interferon titers were observed in CVB3m-infected mice treated with anti-asialo 
      GM1 antiserum as compared with normal CVB3m-infected mice. Measurements of 
      splenic NK cell activity in mice inoculated with doses of 10(2) to 10(8) PFU of 
      CVB3m per mouse or UV-irradiated virus suggest that replication of CVB3m is 
      required for NK cell activation. An amyocarditic variant of CVB3m (ts5R) was 
      shown to replicate in heart tissues and to elicit NK cell activity comparable to 
      that elicited by CVB3m. Therefore, the data suggest that NK cell activation 
      depends on virus replication and that these cells provide some protection against 
      CVB3m-induced myocarditis by limiting virus replication in heart tissues.
FAU - Godeny, E K
AU  - Godeny EK
FAU - Gauntt, C J
AU  - Gauntt CJ
LA  - eng
GR  - HL-21047/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Antibodies)
RN  - 0 (Glycosphingolipids)
RN  - 37758-47-7 (G(M1) Ganglioside)
RN  - 71012-19-6 (asialo GM1 ganglioside)
SB  - IM
MH  - Animals
MH  - Antibodies/immunology
MH  - Coxsackievirus Infections/*immunology
MH  - Enterovirus B, Human/physiology
MH  - *G(M1) Ganglioside
MH  - Glycosphingolipids/immunology
MH  - Killer Cells, Natural/*immunology
MH  - Male
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Myocarditis/etiology/*immunology
MH  - Virus Replication
EDAT- 1986/09/01 00:00
MHDA- 1986/09/01 00:01
CRDT- 1986/09/01 00:00
PHST- 1986/09/01 00:00 [pubmed]
PHST- 1986/09/01 00:01 [medline]
PHST- 1986/09/01 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1986 Sep 1;137(5):1695-702.