PMID- 30181047
OWN - NLM
STAT- MEDLINE
DCOM- 20181211
LR  - 20181211
IS  - 1873-2518 (Electronic)
IS  - 0264-410X (Linking)
VI  - 36
IP  - 41
DP  - 2018 Oct 1
TI  - Characterization of a multi-epitope peptide with selective MHC-binding 
      capabilities encapsulated in PLGA nanoparticles as a novel vaccine candidate 
      against Toxoplasma gondii infection.
PG  - 6124-6132
LID - S0264-410X(18)31208-8 [pii]
LID - 10.1016/j.vaccine.2018.08.068 [doi]
AB  - No effective human vaccine against Toxoplasma gondii (T. gondii) has yet been 
      developed; however, a protective vaccine using immunogenic peptides in a safe 
      delivery vehicle system offers promise. Here, we employed bioinformatics to 
      design a multimeric recombinant T. gondii vaccine using predicted T and B cell 
      epitopes of SAG1, AMA1, ROP2, and GRA4 proteins based on their binding 
      capabilities to common major histocompatibility complex (MHC) molecules. 
      Furthermore, we encapsulated the expressed protein in poly lactic-co-glycolic 
      acid (PLGA) nanoparticles as a delivery vehicle and also used alum as an adjuvant 
      to determine the vaccine potency of this multimeric antigen. BALB/c mice were 
      vaccinated and then challenged with T. gondii RH strain, and the survival rate 
      and cytokine profiles were studied. Mice vaccinated with the multi-epitope-based 
      vaccine, both with and without PLGA, had greater Th1 immune responses, survival 
      rates, specific antibody titers, and IFN-gamma and IL-2 levels than controls, while 
      the alum-adsorbed vaccine stimulated a Th2-type humoral immune response.
CI  - Copyright (c) 2018 Elsevier Ltd. All rights reserved.
FAU - Roozbehani, Mona
AU  - Roozbehani M
AD  - Department of Parasitology and Mycology, School of Medicine, Iran University of 
      Medical Sciences, Tehran, Iran.
FAU - Falak, Reza
AU  - Falak R
AD  - Immunology Research Center, Iran University of Medical Sciences, Tehran, Iran; 
      Department of Immunology, School of Medicine, Iran University of Medical 
      Sciences, Tehran, Iran.
FAU - Mohammadi, Mohsen
AU  - Mohammadi M
AD  - Persian Gulf Marine Biotechnology Research Center, Persian Gulf Biomedical 
      Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, 
      Iran.
FAU - Hemphill, Andrew
AU  - Hemphill A
AD  - Institute of Parasitology, Vetsuisse Faculty, University of Bern, Bern, 
      Switzerland.
FAU - Razmjou, Elham
AU  - Razmjou E
AD  - Department of Parasitology and Mycology, School of Medicine, Iran University of 
      Medical Sciences, Tehran, Iran.
FAU - Meamar, Ahmad Reza
AU  - Meamar AR
AD  - Department of Parasitology and Mycology, School of Medicine, Iran University of 
      Medical Sciences, Tehran, Iran.
FAU - Masoori, Leila
AU  - Masoori L
AD  - Department of Parasitology and Mycology, School of Medicine, Iran University of 
      Medical Sciences, Tehran, Iran.
FAU - Khoshmirsafa, Majid
AU  - Khoshmirsafa M
AD  - Immunology Research Center, Iran University of Medical Sciences, Tehran, Iran; 
      Department of Immunology, School of Medicine, Iran University of Medical 
      Sciences, Tehran, Iran.
FAU - Moradi, Maryam
AU  - Moradi M
AD  - Department of Parasitology and Mycology, School of Medicine, Iran University of 
      Medical Sciences, Tehran, Iran.
FAU - Gharavi, Mohammad Javad
AU  - Gharavi MJ
AD  - Department of Parasitology, Faculty of Allied Medicine, Iran University of 
      Medical Sciences, Tehran, Iran. Electronic address: gharavi.mj@iums.ac.ir.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180901
PL  - Netherlands
TA  - Vaccine
JT  - Vaccine
JID - 8406899
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (Antibodies, Protozoan)
RN  - 0 (Antigens, Protozoan)
RN  - 0 (Interleukin-2)
RN  - 0 (Peptides)
RN  - 0 (Protozoan Proteins)
RN  - 0 (Protozoan Vaccines)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Adjuvants, Immunologic
MH  - Animals
MH  - Antibodies, Protozoan/immunology
MH  - Antigens, Protozoan/*immunology
MH  - Computational Biology
MH  - Female
MH  - Immunity, Humoral/physiology
MH  - Interferon-gamma/metabolism
MH  - Interleukin-2/metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Nanoparticles/*chemistry
MH  - Peptides/*immunology
MH  - Polylactic Acid-Polyglycolic Acid Copolymer/*chemistry
MH  - Protozoan Proteins/immunology
MH  - Protozoan Vaccines/*immunology/*therapeutic use
MH  - Toxoplasma/*immunology/*pathogenicity
OTO - NOTNLM
OT  - Multi-epitope
OT  - PLGA nanoparticle
OT  - Toxoplasma gondii
OT  - Vaccine
EDAT- 2018/09/06 06:00
MHDA- 2018/12/12 06:00
CRDT- 2018/09/06 06:00
PHST- 2018/06/28 00:00 [received]
PHST- 2018/08/21 00:00 [revised]
PHST- 2018/08/28 00:00 [accepted]
PHST- 2018/09/06 06:00 [pubmed]
PHST- 2018/12/12 06:00 [medline]
PHST- 2018/09/06 06:00 [entrez]
AID - S0264-410X(18)31208-8 [pii]
AID - 10.1016/j.vaccine.2018.08.068 [doi]
PST - ppublish
SO  - Vaccine. 2018 Oct 1;36(41):6124-6132. doi: 10.1016/j.vaccine.2018.08.068. Epub 
      2018 Sep 1.