PMID- 30184779
OWN - NLM
STAT- MEDLINE
DCOM- 20181211
LR  - 20181211
IS  - 1873-0191 (Electronic)
IS  - 0928-4931 (Linking)
VI  - 92
DP  - 2018 Nov 1
TI  - Fast dissolving moxifloxacin hydrochloride antibiotic drug from electrospun 
      Eudragit L-100 nonwoven nanofibrous Mats.
PG  - 526-539
LID - S0928-4931(17)34070-5 [pii]
LID - 10.1016/j.msec.2018.06.031 [doi]
AB  - Antimicrobial electrospun nonwoven Eudragit L-100 nanofibrous mats containing 
      Moxifloxacin hydrochloride (MOX-HCL) were fabricated for fast dissolving drug 
      delivery systems (DDSs) associated with wound infection. The morphological 
      characterization of nanofibers using ESEM revealed that the average diameter of 
      non-woven nanofibrous mats ranges 200-600 nm. The nanofiber showed cylindrical 
      shape with crack on the surface. Differential scanning calorimetric (DSC) and 
      Wide Angle X-ray diffraction (WAXRD) demonstrate that the drug exists in an 
      amorphous state in the nanofibers. Nanofibrous mats were also tested for 
      mechanical strength, contact angle, swelling assay, haemolysis and disintegration 
      test. In vitro disintegration tests demonstrated that the dissolution of Eudragit 
      L-100 fiber mats was within 25 s which was higher compared to the pure drug. The 
      Eudragit nanofibers showed pH-dependent drug release profiles, with slow release 
      at pH 1.2 and burst release (around 30 s) at pH 6.8. The in-vitro quantitative 
      and qualitative antimicrobial assay showed that the developed Eudragit L-100 
      nanofibrous mats with MOX-HCL concentration of 1%, 5% and 15 wt% exhibited 
      antibacterial activities against both gram positive (Staphylococcus aureus) and 
      gram negative (Escherichia coli) bacteria. The in-vitro cytotoxicity assay using 
      mouse fibroblast NIH/3T3 cells demonstrated significant biocompatibility of 
      nanofiber mats. As per the results of biological evaluation, Eudragit L-100 
      nanofibrous mats with 1wt% MOX-HCL could be a suitable substrate for biomedical 
      applications. Eudragit L-100 nanofibrous mats containing Moxifloxacin 
      hydrochloride (MOX-HCL) showed immediate DDSs for localized drug release in the 
      wound infection at slightly acidic or alkaline conditions where faster drug 
      release rate is required for wound healing.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Giram, Prabhanjan S
AU  - Giram PS
AD  - Polymer Science and Engineering Division, CSIR-National Chemical Laboratory, Pune 
      411008, India; Academy of Scientific and Innovative Research, New Delhi, India.
FAU - Shitole, Ajinkya
AU  - Shitole A
AD  - Symbiosis School of Biological sciences, Pune 412115, India.
FAU - Nande, Smita S
AU  - Nande SS
AD  - Polymer Science and Engineering Division, CSIR-National Chemical Laboratory, Pune 
      411008, India; Academy of Scientific and Innovative Research, New Delhi, India.
FAU - Sharma, Neeti
AU  - Sharma N
AD  - Symbiosis School of Biological sciences, Pune 412115, India.
FAU - Garnaik, Baijayantimala
AU  - Garnaik B
AD  - Polymer Science and Engineering Division, CSIR-National Chemical Laboratory, Pune 
      411008, India; Academy of Scientific and Innovative Research, New Delhi, India. 
      Electronic address: b.garnaik@ncl.res.in.
LA  - eng
PT  - Journal Article
DEP - 20180625
PL  - Netherlands
TA  - Mater Sci Eng C Mater Biol Appl
JT  - Materials science & engineering. C, Materials for biological applications
JID - 101484109
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Delayed-Action Preparations)
RN  - 0 (Polymethacrylic Acids)
RN  - 25086-15-1 (methylmethacrylate-methacrylic acid copolymer)
RN  - U188XYD42P (Moxifloxacin)
SB  - IM
MH  - Animals
MH  - *Anti-Bacterial Agents/chemistry/pharmacokinetics/pharmacology
MH  - Delayed-Action Preparations/chemistry/pharmacokinetics/pharmacology
MH  - Escherichia coli/*growth & development
MH  - Fibroblasts/cytology/*metabolism
MH  - Hydrogen-Ion Concentration
MH  - *Materials Testing
MH  - Mice
MH  - *Moxifloxacin/chemistry/pharmacokinetics/pharmacology
MH  - NIH 3T3 Cells
MH  - Nanofibers/*chemistry
MH  - *Polymethacrylic Acids/chemistry/pharmacokinetics/pharmacology
MH  - Staphylococcus aureus/*growth & development
OTO - NOTNLM
OT  - Antimicrobial
OT  - Drug delivery
OT  - Electrospinning
OT  - Eudragit
OT  - Moxifloxacin hydrochloride
OT  - Nonwoven nanofibrous mats
EDAT- 2018/09/07 06:00
MHDA- 2018/12/12 06:00
CRDT- 2018/09/07 06:00
PHST- 2017/10/10 00:00 [received]
PHST- 2018/06/01 00:00 [revised]
PHST- 2018/06/13 00:00 [accepted]
PHST- 2018/09/07 06:00 [entrez]
PHST- 2018/09/07 06:00 [pubmed]
PHST- 2018/12/12 06:00 [medline]
AID - S0928-4931(17)34070-5 [pii]
AID - 10.1016/j.msec.2018.06.031 [doi]
PST - ppublish
SO  - Mater Sci Eng C Mater Biol Appl. 2018 Nov 1;92:526-539. doi: 
      10.1016/j.msec.2018.06.031. Epub 2018 Jun 25.