PMID- 30186460
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 16
IP  - 3
DP  - 2018 Sep
TI  - In vitro study on human umbilical cord mesenchymal stem cells transfected with 
      lentivirus-mediated hNIS-EGFP dual reporter gene and co-labeled with 
      superparamagnetic iron oxide.
PG  - 2208-2218
LID - 10.3892/etm.2018.6505 [doi]
AB  - The aim of the present study was to establish a stem cell line for multi-mode 
      imaging (in vivo fluorescence imaging, magnetic resonance imaging and (99m)Tc 
      single-photon emission computed tomography) and to study the biological activity, 
      stemness, proliferative activity and differentiation ability of superparamagnetic 
      iron oxide (SPIO), human sodium/iodide symporter (hNIS) and enhanced green 
      fluorescent protein (EGFP) co-labeled human umbilical cord mesenchymal stem cells 
      (hUCMSCs). The EGFP reporter gene was selected to indirectly reflect the 
      expression of target gene hNIS, and hUCMSCs were re-transfected with the 
      successfully constructed recombinant plasmid pCMV-NIS-EF1-GFP-PGK-puro. When a 
      stem cell line stably expressing hNIS and EGFP was obtained, the cells were 
      incubated with 30 microg/ml SPIO to obtain hNIS, EGFP and SPIO co-labeled stem cells. 
      The protein expressions of hNIS and EGFP were identified using western blot 
      analysis, and the protein function of hNIS was identified by (125)I influx and 
      (125)I efflux experiments. hNIS-EGFP-hUCMSCs were labeled with SPIO under the 
      mediation of poly-L-lysine, and SPIO, hNIS and EGFP co-labeled hUCMSCs were 
      established successfully. Staining with Prussian blue confirmed that 98% of cells 
      were successfully labeled with SPIO. Western blotting results demonstrated 
      positive hNIS and EGFP protein expression levels, and (125)I influx and (125)I 
      efflux experiments confirmed that the protein function of hUCMSCs after 
      expressing hNIS was normal. The uptake of (125)I was higher in cell lines 
      hNIS-EGFP-hUCMSCs than in control hUCMSCs (fold change: 16.43+/-2.30 times; 
      P<0.05). The stemness of hNIS-EGFP-hUCMSCs was found to be slightly decreased but 
      not statistically significant; the overall characteristics of stem cells remained 
      unchanged. The assessments of adipogenic and osteogenic differentiation suggest 
      that hNIS-EGFP-hUCMSCs have no significantly different characteristics compared 
      with primary hUCMSCs.
FAU - Meng, Yu
AU  - Meng Y
AD  - Department of Nephrology, The First Hospital Affiliated to Jinan University, 
      Guangzhou, Guangdong 510630, P.R. China.
FAU - Liu, Huanhuan
AU  - Liu H
AD  - Department of Nephrology, The First Hospital Affiliated to Jinan University, 
      Guangzhou, Guangdong 510630, P.R. China.
FAU - Bian, Ning
AU  - Bian N
AD  - Department of Cardiovascular Medicine, The First Hospital Affiliated to Jinan 
      University, Guangzhou, Guangdong 510630, P.R. China.
FAU - Gong, Jian
AU  - Gong J
AD  - Department of Nuclear Medicine, The First Hospital Affiliated to Jinan 
      University, Guangzhou, Guangdong 510630, P.R. China.
FAU - Zhong, Xing
AU  - Zhong X
AD  - Department of Nuclear Medicine, The First Hospital Affiliated to Jinan 
      University, Guangzhou, Guangdong 510630, P.R. China.
FAU - Huang, Chunrong
AU  - Huang C
AD  - Department of Nephrology, The First Hospital Affiliated to Jinan University, 
      Guangzhou, Guangdong 510630, P.R. China.
FAU - Liang, Wenxue
AU  - Liang W
AD  - Department of Nephrology, The First Hospital Affiliated to Jinan University, 
      Guangzhou, Guangdong 510630, P.R. China.
FAU - Xu, Hao
AU  - Xu H
AD  - Department of Nephrology, The First Hospital Affiliated to Jinan University, 
      Guangzhou, Guangdong 510630, P.R. China.
AD  - Department of Nuclear Medicine, The First Hospital Affiliated to Jinan 
      University, Guangzhou, Guangdong 510630, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20180723
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC6122337
OTO - NOTNLM
OT  - enhanced green fluorescent protein
OT  - human sodium/iodide symporter
OT  - mesenchymal stem cells
OT  - superparamagnetic iron oxide
EDAT- 2018/09/07 06:00
MHDA- 2018/09/07 06:01
PMCR- 2018/07/23
CRDT- 2018/09/07 06:00
PHST- 2016/04/30 00:00 [received]
PHST- 2017/05/05 00:00 [accepted]
PHST- 2018/09/07 06:00 [entrez]
PHST- 2018/09/07 06:00 [pubmed]
PHST- 2018/09/07 06:01 [medline]
PHST- 2018/07/23 00:00 [pmc-release]
AID - ETM-0-0-6505 [pii]
AID - 10.3892/etm.2018.6505 [doi]
PST - ppublish
SO  - Exp Ther Med. 2018 Sep;16(3):2208-2218. doi: 10.3892/etm.2018.6505. Epub 2018 Jul 
      23.