PMID- 30186504
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 16
IP  - 3
DP  - 2018 Sep
TI  - MicroRNA-126 accelerates IgE-mediated mast cell degranulation associated with the 
      PI3K/Akt signaling pathway by promoting Ca(2+) influx.
PG  - 2763-2769
LID - 10.3892/etm.2018.6510 [doi]
AB  - Mast cells (MCs) have been reported to serve a crucial role in allergic diseases, 
      including asthma, allergic rhinitis and anaphylaxis. A previous study revealed 
      that microRNA-126 (miR-126) was associated with airway hyperresponsiveness 
      induced by house dust mites, however the molecular mechanisms were unclear. The 
      present study aimed to investigate the effect of miR-126 on immunoglobulin E 
      (IgE)-regulated MC degranulation and explore its underlying mechanisms. miR-126 
      expression was quantified using a rat model in vivo and in rat peritoneal mast 
      cells (RPMCs) in vitro. Overexpression or downregulation of miR-126 was 
      established by transfection with miR-126 mimics or miR-126 inhibitors and MC 
      degranulation was subsequently evaluated. The effect of miR-126 on protein kinase 
      B (Akt) and phosphorylated Akt protein expression was examined by western blot 
      analysis. The phosphoinositide 3-kinase (PI3K) inhibitor (LY294002) was used to 
      determine the role of the PI3K/Akt signaling pathway. In addition, cytosolic 
      calcium (Ca(2+)) levels were measured by a fura-2 assay. The results demonstrated 
      that miR-126 expression was upregulated in the ear tissues of rats with allergic 
      contact dermatitis and IgE-activated MCs. The overexpression of miR-126 in RPMCs 
      was established following miR-126 mimic transfection. The release of 
      beta-hexosaminidase and histamine, markers of MC degranulation, were significantly 
      increased in cells with miR-126 overexpression. The phosphorylation of Akt was 
      significantly increased following transfection with miR-126 mimics in stimulated 
      cells, however the signaling activation was abrogated by LY294002. In addition, 
      Ca(2+) influx was significantly promoted in stimulated RPMCs overexpressing 
      miR-126. These results indicate that miR-126 accelerated IgE-mediated MC 
      degranulation associated with the PI3K/Akt signaling pathway by promoting Ca(2+) 
      influx. This suggests that miR-126 may be a promising therapeutic target for the 
      treatment of allergic skin diseases.
FAU - Bao, Yuan
AU  - Bao Y
AD  - Department of Network Medicine, The Central Hospital of Wuhan, Tongji Medical 
      College, Huazhong University of Science and Technology, Wuhan, Hubei 430014, P.R. 
      China.
FAU - Wang, Song
AU  - Wang S
AD  - Department of Massage, Wuhan Hospital of Traditional Chinese Medicine, Wuhan, 
      Hubei 430014, P.R. China.
FAU - Gao, Yang
AU  - Gao Y
AD  - Department of Massage, Wuhan Hospital of Traditional Chinese Medicine, Wuhan, 
      Hubei 430014, P.R. China.
FAU - Zhang, Wen
AU  - Zhang W
AD  - Department of Encephalopathy (I), Wuhan Hospital of Traditional Chinese Medicine, 
      Wuhan, Hubei 430014, P.R. China.
FAU - Jin, Haitao
AU  - Jin H
AD  - Department of Encephalopathy (II), Wuhan Hospital of Traditional Chinese 
      Medicine, Wuhan, Hubei 430014, P.R. China.
FAU - Yang, Yang
AU  - Yang Y
AD  - Department of Network Medicine, The Central Hospital of Wuhan, Tongji Medical 
      College, Huazhong University of Science and Technology, Wuhan, Hubei 430014, P.R. 
      China.
FAU - Li, Jiangyu
AU  - Li J
AD  - Department of Gerontology, Wuhan Hospital of Traditional Chinese Medicine, Wuhan, 
      Hubei 430014, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20180723
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC6122504
OTO - NOTNLM
OT  - Ca2+ influx
OT  - allergic skin diseases
OT  - mast cells
OT  - microRNA-126
OT  - phosphoinositide 3-kinase/protein kinase B signaling pathway
EDAT- 2018/09/07 06:00
MHDA- 2018/09/07 06:01
PMCR- 2018/07/23
CRDT- 2018/09/07 06:00
PHST- 2017/08/24 00:00 [received]
PHST- 2018/06/15 00:00 [accepted]
PHST- 2018/09/07 06:00 [entrez]
PHST- 2018/09/07 06:00 [pubmed]
PHST- 2018/09/07 06:01 [medline]
PHST- 2018/07/23 00:00 [pmc-release]
AID - ETM-0-0-6510 [pii]
AID - 10.3892/etm.2018.6510 [doi]
PST - ppublish
SO  - Exp Ther Med. 2018 Sep;16(3):2763-2769. doi: 10.3892/etm.2018.6510. Epub 2018 Jul 
      23.