PMID- 30189852
OWN - NLM
STAT- MEDLINE
DCOM- 20190819
LR  - 20231213
IS  - 1471-2202 (Electronic)
IS  - 1471-2202 (Linking)
VI  - 19
IP  - 1
DP  - 2018 Sep 6
TI  - Involvement of Akt/CREB signaling pathways in the protective effect of EPA 
      against interleukin-1beta-induced cytotoxicity and BDNF down-regulation in cultured 
      rat hippocampal neurons.
PG  - 52
LID - 10.1186/s12868-018-0455-7 [doi]
LID - 52
AB  - BACKGROUND: Our published data have indicated that the omega-3 polyunsaturated 
      fatty acid eicosapentaenoic acid (EPA) provides beneficial effects by attenuating 
      neuronal damage induced by interleukin-1beta (IL-1beta), and up-regulation of the 
      expression of brain-derived neurotrophic factor (BDNF) represents a crucial part 
      in the neuroprotective effect of EPA. However, the mechanisms of how EPA 
      regulates BDNF expression remains incompletely understood. The present study 
      investigated the role of Akt/CREB signaling in the effect of EPA on BDNF 
      expression and its neuroprotective effect. RESULTS: The present results showed 
      that IL-1beta reduced hippocampal neuronal viability and that EPA showed a 
      concentration-dependent neuroprotective effect, but the neuroprotective effects 
      of EPA were abolished by inhibition of Akt using KRX-0401, an inhibitor of Akt. 
      Treatment of hippocampal neurons with EPA also ameliorated the decrease in Akt 
      and CREB phosphorylation induced by IL-1beta and BDNF down-regulation mediated by 
      IL-1beta. However, inhibition of Akt reversed the effect of EPA on levels of p-Akt, 
      p-CREB, and BDNF. CONCLUSIONS: Our data indicate that EPA elicited 
      neuroprotection toward IL-1beta-induced cell damage and BDNF decrease and that its 
      effects potentially occurred via the Akt/CREB signaling pathway.
FAU - Dong, YiLong
AU  - Dong Y
AUID- ORCID: 0000-0002-6078-1924
AD  - School of Medicine, Yunnan University, 2 Cuihu Bei Road, Kunming, 650091, Yunnan, 
      People's Republic of China. dongyilongmail@126.com.
FAU - Pu, KangJing
AU  - Pu K
AD  - School of Medicine, Yunnan University, 2 Cuihu Bei Road, Kunming, 650091, Yunnan, 
      People's Republic of China.
FAU - Duan, WenJing
AU  - Duan W
AD  - The First Affiliated Hospital of Kunming Medical University, 295 Xichang Road, 
      Kunming, 650031, Yunnan, People's Republic of China.
FAU - Chen, HuiCheng
AU  - Chen H
AD  - School of Medicine, Yunnan University, 2 Cuihu Bei Road, Kunming, 650091, Yunnan, 
      People's Republic of China.
FAU - Chen, LiXing
AU  - Chen L
AD  - The First Affiliated Hospital of Kunming Medical University, 295 Xichang Road, 
      Kunming, 650031, Yunnan, People's Republic of China.
FAU - Wang, YanMei
AU  - Wang Y
AD  - The First Affiliated Hospital of Kunming Medical University, 295 Xichang Road, 
      Kunming, 650031, Yunnan, People's Republic of China. yaner6922@126.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180906
PL  - England
TA  - BMC Neurosci
JT  - BMC neuroscience
JID - 100966986
RN  - 0 (Bdnf protein, rat)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Creb1 protein, rat)
RN  - 0 (Cyclic AMP Response Element-Binding Protein)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Neuroprotective Agents)
RN  - AAN7QOV9EA (Eicosapentaenoic Acid)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Cyclic AMP Response Element-Binding Protein/*metabolism
MH  - Dose-Response Relationship, Drug
MH  - Down-Regulation/drug effects
MH  - Eicosapentaenoic Acid/*pharmacology
MH  - Hippocampus/*drug effects/metabolism
MH  - Interleukin-1beta/*metabolism/toxicity
MH  - Neurons/drug effects/metabolism
MH  - Neuroprotective Agents/*pharmacology
MH  - Primary Cell Culture
MH  - Proto-Oncogene Proteins c-akt/antagonists & inhibitors/*metabolism
MH  - Rats, Sprague-Dawley
MH  - Signal Transduction/drug effects
PMC - PMC6128001
OTO - NOTNLM
OT  - *Akt
OT  - *Brain-derived neurotrophic factor
OT  - *Eicosapentaenoic acid
OT  - *Interleukin-1beta
EDAT- 2018/09/08 06:00
MHDA- 2019/08/20 06:00
PMCR- 2018/09/06
CRDT- 2018/09/08 06:00
PHST- 2018/03/19 00:00 [received]
PHST- 2018/08/31 00:00 [accepted]
PHST- 2018/09/08 06:00 [entrez]
PHST- 2018/09/08 06:00 [pubmed]
PHST- 2019/08/20 06:00 [medline]
PHST- 2018/09/06 00:00 [pmc-release]
AID - 10.1186/s12868-018-0455-7 [pii]
AID - 455 [pii]
AID - 10.1186/s12868-018-0455-7 [doi]
PST - epublish
SO  - BMC Neurosci. 2018 Sep 6;19(1):52. doi: 10.1186/s12868-018-0455-7.