PMID- 30190594
OWN - NLM
STAT- MEDLINE
DCOM- 20191031
LR  - 20191031
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 8
IP  - 1
DP  - 2018 Sep 6
TI  - Viral communities associated with porcine respiratory disease complex in 
      intensive commercial farms in Sichuan province, China.
PG  - 13341
LID - 10.1038/s41598-018-31554-8 [doi]
LID - 13341
AB  - Porcine respiratory disease complex (PRDC), a common piglet disease, causes 
      substantive economic losses in pig farming. To investigate the viral diversity 
      associated with PRDC, the viral communities in serum and nasal swabs from 26 
      PRDC-affected piglets were investigated using metagenomics. By deep sequencing 
      and de novo assembly, 17 viruses were identified in two pooled libraries (16 
      viruses from serum, nine from nasal swabs). Porcine circovirus (PCV)-2, porcine 
      reproductive and respiratory syndrome virus (PRRSV) and pseudorabies virus, all 
      commonly associated with PRDC, were identified in the two pooled samples by 
      metagenomics, but most viruses comprised small linear and circular DNAs (e.g. 
      parvoviruses, bocaviruses and circoviruses). PCR was used to compare the 
      detection rates of each virus in the serum samples from 36 PRDC-affected piglets 
      versus 38 location-matched clinically healthy controls. The average virus 
      category per sample was 6.81 for the PRDC-affected piglets and 4.09 for the 
      controls. Single or co-infections with PCV-2 or PRRSV had very high detection 
      rates in the PRDC-affected piglets. Interestingly, porcine parvovirus (PPV)-2, 
      PPV-3, PPV-6 and torque teno sus virus 1a were significantly associated with 
      PRDC. These results illustrate the complexity of viral communities in the 
      PRDC-affected piglets and highlight the candidate viruses associated with it.
FAU - Qin, Sinan
AU  - Qin S
AD  - College of Life Science and Technology, Southwest Minzu University, Chengdu, 
      610041, China.
FAU - Ruan, Wenqiang
AU  - Ruan W
AD  - College of Life Science and Technology, Southwest Minzu University, Chengdu, 
      610041, China.
FAU - Yue, Hua
AU  - Yue H
AD  - College of Life Science and Technology, Southwest Minzu University, Chengdu, 
      610041, China. yhua900@163.com.
AD  - Key laboratory of Ministry of Education and Sichuan Province for Qinghai-Tibetan 
      Plateau Animal Genetic Resource Reservation and Utilization, Chengdu, 610041, 
      China. yhua900@163.com.
AD  - Animal Disease Prevention and Control Innovation Team in the Qinghai-Tibetan 
      Plateau of State Ethnic Affairs Commission, Chengdu, 610041, China. 
      yhua900@163.com.
FAU - Tang, Cheng
AU  - Tang C
AD  - College of Life Science and Technology, Southwest Minzu University, Chengdu, 
      610041, China.
AD  - Key laboratory of Ministry of Education and Sichuan Province for Qinghai-Tibetan 
      Plateau Animal Genetic Resource Reservation and Utilization, Chengdu, 610041, 
      China.
AD  - Animal Disease Prevention and Control Innovation Team in the Qinghai-Tibetan 
      Plateau of State Ethnic Affairs Commission, Chengdu, 610041, China.
FAU - Zhou, Kelei
AU  - Zhou K
AD  - College of Life Science and Technology, Southwest Minzu University, Chengdu, 
      610041, China.
FAU - Zhang, Bin
AU  - Zhang B
AD  - College of Life Science and Technology, Southwest Minzu University, Chengdu, 
      610041, China. binovy@sina.com.
AD  - Key laboratory of Ministry of Education and Sichuan Province for Qinghai-Tibetan 
      Plateau Animal Genetic Resource Reservation and Utilization, Chengdu, 610041, 
      China. binovy@sina.com.
AD  - Animal Disease Prevention and Control Innovation Team in the Qinghai-Tibetan 
      Plateau of State Ethnic Affairs Commission, Chengdu, 610041, China. 
      binovy@sina.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180906
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
SB  - IM
MH  - Animals
MH  - China
MH  - Circovirus/*genetics
MH  - Farms
MH  - Herpesvirus 1, Suid/*genetics
MH  - Metagenomics
MH  - Parvovirus, Porcine/*genetics
MH  - Porcine respiratory and reproductive syndrome virus/*genetics
MH  - *Respiratory Tract Diseases/epidemiology/genetics/virology
MH  - Swine
MH  - *Swine Diseases/epidemiology/genetics/virology
PMC - PMC6127300
COIS- The authors declare no competing interests.
EDAT- 2018/09/08 06:00
MHDA- 2019/11/02 06:00
PMCR- 2018/09/06
CRDT- 2018/09/08 06:00
PHST- 2018/03/12 00:00 [received]
PHST- 2018/08/22 00:00 [accepted]
PHST- 2018/09/08 06:00 [entrez]
PHST- 2018/09/08 06:00 [pubmed]
PHST- 2019/11/02 06:00 [medline]
PHST- 2018/09/06 00:00 [pmc-release]
AID - 10.1038/s41598-018-31554-8 [pii]
AID - 31554 [pii]
AID - 10.1038/s41598-018-31554-8 [doi]
PST - epublish
SO  - Sci Rep. 2018 Sep 6;8(1):13341. doi: 10.1038/s41598-018-31554-8.