PMID- 30191441
OWN - NLM
STAT- MEDLINE
DCOM- 20190529
LR  - 20200309
IS  - 1559-0100 (Electronic)
IS  - 1355-008X (Print)
IS  - 1355-008X (Linking)
VI  - 63
IP  - 1
DP  - 2019 Jan
TI  - Oral glucose load and mixed meal feeding lowers testosterone levels in healthy 
      eugonadal men.
PG  - 149-156
LID - 10.1007/s12020-018-1741-y [doi]
AB  - PURPOSE: Precise evaluation of serum testosterone levels is important in making 
      an accurate diagnosis of androgen deficiency. Recent practice guidelines on male 
      androgen deficiency recommend that testosterone be measured in the morning while 
      fasting. Although there is ample evidence regarding morning measurement of 
      testosterone, studies that evaluated the effect of glucose load or meals were 
      limited by inclusion of hypogonadal or diabetic men, and measurement of 
      testosterone was not performed using mass spectrometry. METHODS: Sixty men (23-97 
      years) without pre-diabetes or diabetes who had normal total testosterone (TT) 
      levels underwent either an oral glucose tolerance test (OGTT) or a mixed meal 
      tolerance test (MMTT) after an overnight fast. Serum samples were collected 
      before and at regular intervals for 2 h (OGTT cohort) or 3 h (MMTT cohort). TT 
      was measured by LC-MS/MS. LH and prolactin were also measured. RESULTS: TT 
      decreased after a glucose load (mean drop at nadir = 100 ng/dL) and after a mixed 
      meal (drop at nadir = 123 ng/dL). Approximately 11% of men undergoing OGTT and 
      56% undergoing MMTT experienced a transient decrease in TT below 300 ng/dL, the 
      lower normal limit. Testosterone started declining 20 min into the tests, with 
      average maximum decline at 60 min. Most men still had TT lower than baseline at 
      120 min. This effect was independent of changes in LH or prolactin. CONCLUSION: A 
      glucose load or a mixed meal transiently, but significantly, lowers TT levels in 
      healthy, non-diabetic eugonadal men. These findings support the recommendations 
      that measurement of serum testosterone to diagnose androgen deficiency should be 
      performed while fasting.
FAU - Gagliano-Juca, Thiago
AU  - Gagliano-Juca T
AUID- ORCID: 0000-0002-2960-1059
AD  - Research Program in Men's Health: Aging and Metabolism, Brigham and Women's 
      Hospital, Harvard Medical School, Boston, MA, 02115, USA. tgagliano@partners.org.
FAU - Li, Zhuoying
AU  - Li Z
AD  - Research Program in Men's Health: Aging and Metabolism, Brigham and Women's 
      Hospital, Harvard Medical School, Boston, MA, 02115, USA.
FAU - Pencina, Karol M
AU  - Pencina KM
AD  - Research Program in Men's Health: Aging and Metabolism, Brigham and Women's 
      Hospital, Harvard Medical School, Boston, MA, 02115, USA.
FAU - Beleva, Yusnie M
AU  - Beleva YM
AD  - Research Program in Men's Health: Aging and Metabolism, Brigham and Women's 
      Hospital, Harvard Medical School, Boston, MA, 02115, USA.
FAU - Carlson, Olga D
AU  - Carlson OD
AD  - Laboratory of Clinical Investigation, National Institute on Aging, Baltimore, MD, 
      21225, USA.
FAU - Egan, Josephine M
AU  - Egan JM
AD  - Laboratory of Clinical Investigation, National Institute on Aging, Baltimore, MD, 
      21225, USA.
FAU - Basaria, Shehzad
AU  - Basaria S
AD  - Research Program in Men's Health: Aging and Metabolism, Brigham and Women's 
      Hospital, Harvard Medical School, Boston, MA, 02115, USA.
LA  - eng
GR  - Z99 AG999999/Intramural NIH HHS/United States
GR  - 03-AG-N035/AG/NIA NIH HHS/United States
GR  - 15-AG-N074/AG/NIA NIH HHS/United States
PT  - Journal Article
DEP - 20180906
PL  - United States
TA  - Endocrine
JT  - Endocrine
JID - 9434444
RN  - 0 (Blood Glucose)
RN  - 0 (Insulin)
RN  - 3XMK78S47O (Testosterone)
RN  - 9002-62-4 (Prolactin)
RN  - 9002-67-9 (Luteinizing Hormone)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Adult
MH  - Blood Glucose/analysis
MH  - Cohort Studies
MH  - Glucose/*pharmacology
MH  - Glucose Tolerance Test
MH  - *Glycemic Load
MH  - Healthy Volunteers
MH  - Humans
MH  - Insulin/blood
MH  - Longitudinal Studies
MH  - Luteinizing Hormone/blood
MH  - Male
MH  - *Meals
MH  - Middle Aged
MH  - Prolactin/blood
MH  - Testosterone/*blood
MH  - Young Adult
PMC - PMC6445266
MID - NIHMS1010918
OTO - NOTNLM
OT  - Hypogonadism
OT  - LH
OT  - Mixed meal tolerance test
OT  - Oral glucose tolerance test
OT  - Sex hormones
OT  - Testosterone
COIS- Compliance with ethical standards Conflict of interest Dr. Basaria has no 
      conflict of interest related to the current work. He has previously received 
      grant support from Abbott Pharmaceuticals for investigator-initiated studies 
      unrelated to this study and has previously consulted for AbbVie, Eli Lilly, Inc 
      and Regeneron Pharmaceuticals. The other authors declare that they have no 
      conflict of interest.
EDAT- 2018/09/08 06:00
MHDA- 2019/05/30 06:00
PMCR- 2019/04/02
CRDT- 2018/09/08 06:00
PHST- 2018/07/18 00:00 [received]
PHST- 2018/08/29 00:00 [accepted]
PHST- 2018/09/08 06:00 [pubmed]
PHST- 2019/05/30 06:00 [medline]
PHST- 2018/09/08 06:00 [entrez]
PHST- 2019/04/02 00:00 [pmc-release]
AID - 10.1007/s12020-018-1741-y [pii]
AID - 10.1007/s12020-018-1741-y [doi]
PST - ppublish
SO  - Endocrine. 2019 Jan;63(1):149-156. doi: 10.1007/s12020-018-1741-y. Epub 2018 Sep 
      6.