PMID- 30194656
OWN - NLM
STAT- MEDLINE
DCOM- 20190528
LR  - 20210109
IS  - 1861-0293 (Electronic)
IS  - 1340-3443 (Linking)
VI  - 73
IP  - 1
DP  - 2019 Jan
TI  - Geraniin promotes osteogenic differentiation of bone marrow mesenchymal stem 
      cells (BMSCs) via activating beta-catenin: a comparative study between BMSCs from 
      normal and osteoporotic rats.
PG  - 262-272
LID - 10.1007/s11418-018-1242-6 [doi]
AB  - Abnormal osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) 
      has been correlated with the pathogenesis of osteoporosis. Geraniin, a 
      polyphenolic compound isolated from Phyllanthus amarus, is effective in 
      preventing osteoporosis, but the mechanisms of action of geraniin and the impact 
      of osteoporotic condition on drug action are not known. In this study we compared 
      the proliferation and osteoblastic differentiation potential of BMSCs from normal 
      rats with that from osteoporotic rats, and examined the responses of both BMSCs 
      to geraniin in parallel. BMSCs of rats subjected to ovariectomy or sham operation 
      were isolated and treated with geraniin. Cell proliferation was measured by CCK-8 
      assay. Osteoblastic differentiation was quantified by Alizarin Red S staining and 
      alkaline phosphatase assay. Nuclear translocation of beta-catenin was monitored by 
      immunofluorescent staining. Expression of beta-catenin was determined by Western 
      blot and quantitative real-time polymerase chain reaction. Results showed that 
      the proliferation and osteoblast formation of osteoporotic BMSCs decreased in 
      comparison to that of normal BMSCs. Geraniin enhanced proliferation and 
      osteoblastic differentiation of both BMSCs, but the responses of osteoporotic 
      BMSCs to geraniin were less than those of normal BMSCs. Expression and nuclear 
      accumulation of beta-catenin in osteoporotic BMSCs were found to be diminished. 
      Geraniin increased nuclear translocation and expression of beta-catenin in both 
      BMSCs. This study associated the osteogenic effect of geraniin to activation of 
      Wnt/beta-catenin signaling, and provided rationale for pharmacological investigation 
      of geraniin in osteoporosis prevention and treatment.
FAU - Mo, Jiao
AU  - Mo J
AD  - School of Pharmaceutical Sciences and Yunnan Key Laboratory of Pharmacology for 
      Natural Products, Kunming Medical University, No. 1168 Chunrongxi Road, Chenggong 
      District, Kunming, 650500, Yunnan, People's Republic of China.
FAU - Yang, Renhua
AU  - Yang R
AD  - School of Pharmaceutical Sciences and Yunnan Key Laboratory of Pharmacology for 
      Natural Products, Kunming Medical University, No. 1168 Chunrongxi Road, Chenggong 
      District, Kunming, 650500, Yunnan, People's Republic of China.
FAU - Li, Fan
AU  - Li F
AD  - School of Pharmaceutical Sciences and Yunnan Key Laboratory of Pharmacology for 
      Natural Products, Kunming Medical University, No. 1168 Chunrongxi Road, Chenggong 
      District, Kunming, 650500, Yunnan, People's Republic of China.
FAU - He, Bo
AU  - He B
AD  - School of Pharmaceutical Sciences and Yunnan Key Laboratory of Pharmacology for 
      Natural Products, Kunming Medical University, No. 1168 Chunrongxi Road, Chenggong 
      District, Kunming, 650500, Yunnan, People's Republic of China.
FAU - Zhang, Xiaochao
AU  - Zhang X
AD  - School of Pharmaceutical Sciences and Yunnan Key Laboratory of Pharmacology for 
      Natural Products, Kunming Medical University, No. 1168 Chunrongxi Road, Chenggong 
      District, Kunming, 650500, Yunnan, People's Republic of China.
FAU - Zhao, Yuqin
AU  - Zhao Y
AD  - School of Pharmaceutical Sciences and Yunnan Key Laboratory of Pharmacology for 
      Natural Products, Kunming Medical University, No. 1168 Chunrongxi Road, Chenggong 
      District, Kunming, 650500, Yunnan, People's Republic of China.
FAU - Shen, Zhiqiang
AU  - Shen Z
AD  - School of Pharmaceutical Sciences and Yunnan Key Laboratory of Pharmacology for 
      Natural Products, Kunming Medical University, No. 1168 Chunrongxi Road, Chenggong 
      District, Kunming, 650500, Yunnan, People's Republic of China. 
      shenzhiqiang@kmmu.edu.cn.
FAU - Chen, Peng
AU  - Chen P
AD  - School of Pharmaceutical Sciences and Yunnan Key Laboratory of Pharmacology for 
      Natural Products, Kunming Medical University, No. 1168 Chunrongxi Road, Chenggong 
      District, Kunming, 650500, Yunnan, People's Republic of China. 
      chenpeng@kmmu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20180907
PL  - Japan
TA  - J Nat Med
JT  - Journal of natural medicines
JID - 101518405
RN  - 0 (Glucosides)
RN  - 0 (Hydrolyzable Tannins)
RN  - 0 (beta Catenin)
RN  - 60976-49-0 (Geraniin)
SB  - IM
MH  - Animals
MH  - Cell Differentiation
MH  - Cell Proliferation/drug effects
MH  - Female
MH  - Glucosides/pharmacology/*therapeutic use
MH  - Hydrolyzable Tannins/pharmacology/*therapeutic use
MH  - Mesenchymal Stem Cells/*metabolism
MH  - Osteogenesis/*drug effects
MH  - Osteoporosis/*drug therapy
MH  - Rats
MH  - beta Catenin/*metabolism
OTO - NOTNLM
OT  - Geraniin
OT  - Mesenchymal stem cells
OT  - Osteogenesis
OT  - Osteoporosis
OT  - beta-Catenin
EDAT- 2018/09/09 06:00
MHDA- 2019/05/29 06:00
CRDT- 2018/09/09 06:00
PHST- 2018/06/21 00:00 [received]
PHST- 2018/08/20 00:00 [accepted]
PHST- 2018/09/09 06:00 [pubmed]
PHST- 2019/05/29 06:00 [medline]
PHST- 2018/09/09 06:00 [entrez]
AID - 10.1007/s11418-018-1242-6 [pii]
AID - 10.1007/s11418-018-1242-6 [doi]
PST - ppublish
SO  - J Nat Med. 2019 Jan;73(1):262-272. doi: 10.1007/s11418-018-1242-6. Epub 2018 Sep 
      7.