PMID- 30195883 OWN - NLM STAT- MEDLINE DCOM- 20181105 LR - 20201209 IS - 1618-095X (Electronic) IS - 0944-7113 (Linking) VI - 48 DP - 2018 Sep 15 TI - Pyrus ussuriensis Maxim. leaves extract ameliorates DNCB-induced atopic dermatitis-like symptoms in NC/Nga mice. PG - 76-83 LID - S0944-7113(18)30169-7 [pii] LID - 10.1016/j.phymed.2018.05.006 [doi] AB - PURPOSE: Pyrus ussuriensis Maxim. has been reported to treat the fever, cough, asthma, and chronic skin disease in Korean Medicine. However, there is no scientific evidence for the use of Pyrus ussuriensis Maxim. Leaves (PUL) extract or its mechanism of action in atopic dermatitis (AD). This study was performed to find the potential therapeutic effects of PUL on the progression of AD using in vitro and in vivo experimental models. METHODS: We examined the effects of PUL on the production of nitric oxide (NO) in RAW 264.7, Interleukin 6 (IL-6) and Interleukin 1beta (IL-1beta) in tumor necrosis factor alpha (TNF-alpha) -induced HaCaT cells, respectively. The PUL extract was topically administered to the 2,4-Dinitrochlorobenzene (DNCB) -treated NC/Nga mice. The potential effects of PUL extract were evaluated by measuring the dermatitis score, scratching behavior and serum levels of immunoglobulin E (IgE). The Interleukin 4 (IL-4) and Interleukin 13 (IL-13) cytokines levels were also measured in the splenocytes. In addition, the major components from PUL were analyzed using high performance liquid chromatography (HPLC). RESULTS: PUL extract significantly reduced the level of NO in RAW 264.7 cells, as well as IL-6 and IL-1beta in TNF-alpha-induced HaCaT cells. It also reduced IL-4 and IL-13 levels in splenocytes. In DNCB-treated NC/Nga mice, PUL extract significantly ameliorated the dermatitis severity, scratching tendency and transepidermal water loss (TEWL) compared to the negative control. Also, it normalized skin barriers with decreased production of IgE in mice serum. The arbutin, chlorogenic acid, and rutin were identified as major constituents of the extract by HPLC analysis. These constituents may be involved either alone or together in the regulation of atopic dermatitis. CONCLUSION: Our studies indicate that PUL ameliorates atopic dermatitis-like symptoms by suppressing the proinflammatory cytokines and immune stimuli in both in vitro and in vivo animal models. Therefore, these data suggest that PUL might be an effective natural resource for the treatment of AD. CI - Copyright (c) 2018 The Authors. Published by Elsevier GmbH.. All rights reserved. FAU - Cho, KyoHee AU - Cho K AD - College of Pharmacy, Gachon University, 191, Hambakmoero, Yeonsu-gu, Incheon 21936, Republic of Korea; Gachon Institute of Pharmaceutical Science, Gachon University, 191, Hambakmoe-ro, Yeonsu-gu, Incheon 21936, Republic of Korea. FAU - Parveen, Amna AU - Parveen A AD - College of Pharmacy, Gachon University, 191, Hambakmoero, Yeonsu-gu, Incheon 21936, Republic of Korea; Gachon Institute of Pharmaceutical Science, Gachon University, 191, Hambakmoe-ro, Yeonsu-gu, Incheon 21936, Republic of Korea; Department of Pharmacognosy, Faculty of Pharmaceutical Science, Government College University, Faisalabad, Faisalabad, Pakistan. FAU - Kang, Min Cheol AU - Kang MC AD - College of Pharmacy, Gachon University, 191, Hambakmoero, Yeonsu-gu, Incheon 21936, Republic of Korea; Gachon Institute of Pharmaceutical Science, Gachon University, 191, Hambakmoe-ro, Yeonsu-gu, Incheon 21936, Republic of Korea. FAU - Subedi, Lalita AU - Subedi L AD - College of Pharmacy, Gachon University, 191, Hambakmoero, Yeonsu-gu, Incheon 21936, Republic of Korea; Gachon Institute of Pharmaceutical Science, Gachon University, 191, Hambakmoe-ro, Yeonsu-gu, Incheon 21936, Republic of Korea. FAU - Lee, Jae Hyuk AU - Lee JH AD - College of Pharmacy, Gachon University, 191, Hambakmoero, Yeonsu-gu, Incheon 21936, Republic of Korea; Gachon Institute of Pharmaceutical Science, Gachon University, 191, Hambakmoe-ro, Yeonsu-gu, Incheon 21936, Republic of Korea. FAU - Park, Sun Young AU - Park SY AD - College of Medicine, Gachon University, Incheon 21999, Republic of Korea. FAU - Jin, Mi Rim AU - Jin MR AD - College of Medicine, Gachon University, Incheon 21999, Republic of Korea. FAU - Yoon, Hyeokjun AU - Yoon H AD - Biological and Genetic Resources Assessment Division, National Institute of Biological Resources, 42, Hwangyeong-ro, Seo-gu, Incheon 22689, Republic of Korea. FAU - Son, Youn Kyoung AU - Son YK AD - Biological and Genetic Resources Assessment Division, National Institute of Biological Resources, 42, Hwangyeong-ro, Seo-gu, Incheon 22689, Republic of Korea. FAU - Kim, Sun Yeou AU - Kim SY AD - College of Pharmacy, Gachon University, 191, Hambakmoero, Yeonsu-gu, Incheon 21936, Republic of Korea; Gachon Institute of Pharmaceutical Science, Gachon University, 191, Hambakmoe-ro, Yeonsu-gu, Incheon 21936, Republic of Korea. Electronic address: sunnykim@gachon.ac.kr. LA - eng PT - Journal Article DEP - 20180508 PL - Germany TA - Phytomedicine JT - Phytomedicine : international journal of phytotherapy and phytopharmacology JID - 9438794 RN - 0 (Dinitrochlorobenzene) RN - 0 (Interleukin-13) RN - 0 (Interleukin-1beta) RN - 0 (Interleukin-6) RN - 0 (Plant Extracts) RN - 0 (Tumor Necrosis Factor-alpha) RN - 207137-56-2 (Interleukin-4) RN - 31C4KY9ESH (Nitric Oxide) RN - 37341-29-0 (Immunoglobulin E) SB - IM MH - Animals MH - Cell Line MH - Dermatitis, Atopic/chemically induced/*drug therapy MH - Dinitrochlorobenzene MH - Female MH - Humans MH - Immunoglobulin E/blood MH - Interleukin-13/metabolism MH - Interleukin-1beta/metabolism MH - Interleukin-4/metabolism MH - Interleukin-6/metabolism MH - Mice MH - Mice, Inbred C57BL MH - Nitric Oxide/metabolism MH - Plant Extracts/*pharmacology MH - Plant Leaves/chemistry MH - Pyrus/*chemistry MH - RAW 264.7 Cells MH - Tumor Necrosis Factor-alpha/pharmacology OTO - NOTNLM OT - 2, 4-dinitrochlorobenzene OT - Atopic dermatitis OT - IgE OT - Interleukins OT - NC/Nga mice OT - Pyrus ussuriensis Maxim EDAT- 2018/09/10 06:00 MHDA- 2018/11/06 06:00 CRDT- 2018/09/10 06:00 PHST- 2017/09/19 00:00 [received] PHST- 2018/04/11 00:00 [revised] PHST- 2018/05/07 00:00 [accepted] PHST- 2018/09/10 06:00 [entrez] PHST- 2018/09/10 06:00 [pubmed] PHST- 2018/11/06 06:00 [medline] AID - S0944-7113(18)30169-7 [pii] AID - 10.1016/j.phymed.2018.05.006 [doi] PST - ppublish SO - Phytomedicine. 2018 Sep 15;48:76-83. doi: 10.1016/j.phymed.2018.05.006. Epub 2018 May 8.