PMID- 30196861
OWN - NLM
STAT- MEDLINE
DCOM- 20190207
LR  - 20240229
IS  - 1878-4119 (Electronic)
IS  - 1010-5182 (Linking)
VI  - 46
IP  - 9
DP  - 2018 Sep
TI  - Biofunctionalization of synthetic bone substitutes with angiogenic stem cells: 
      Influence on regeneration of critical-size bone defects in an in vivo murine 
      model.
PG  - 1601-1608
LID - S1010-5182(18)30387-1 [pii]
LID - 10.1016/j.jcms.2018.06.002 [doi]
AB  - PURPOSE: The aim of this study was to investigate the influence of human bone 
      marrow-derived endothelial progenitor cells (EPC) on vascularization and bone 
      regeneration in combination with a bone-substitute material (BSM) in a 
      critical-size bone defect in a murine model. Critical-size bone defects were 
      performed and the defects were filled according to the group membership. 
      MATERIALS AND METHODS: Eighteen rats were randomized in two experimental groups: 
      BSM (BoneCeramic) with/without EPC biofunctionalization, and a control group 
      without BSM and EPC. Calvaria bone defects were performed and the defects were 
      filled according to the group membership. After 8 weeks, qualitative tissue 
      response of newly formed bone mass was analyzed by histology, cone beam CT (CBCT) 
      and micro-CT (muCT) scans. Occurrence of tumor masses due to EPC vascularization 
      in peripheral organs was investigated microscopically in histological slides of 
      liver and kidney. RESULTS: The combination of EPC and BSM showed smaller bone 
      defects in the CT scans and the histological evaluation as the single use of BSM 
      without EPC by trend (p = 0.067). Further, a higher amount of blood vessels could 
      be found in histological slices of BSM in combination with EPC. No inflammatory 
      response or tumor formation could be found. CONCLUSION: These findings confirm 
      the biocompatibility of the used BSM and provide evidence that the combination of 
      BSM with EPC might be effective for bone vascularization and regeneration. Using 
      EPC in augmentation sites might overall lead to faster and better bone 
      regeneration and should be further investigated in future studies.
CI  - Copyright (c) 2018 European Association for Cranio-Maxillo-Facial Surgery. 
      Published by Elsevier Ltd. All rights reserved.
FAU - Beger, Benjamin
AU  - Beger B
AD  - Department of Oral- and Maxillofacial Surgery, (Head: Univ.-Prof. Dr. Dr. B. 
      Al-Nawas), University Medical Center, Augustusplatz 2, 55131, Mainz, Germany. 
      Electronic address: benjamin.beger@unimedizin-mainz.de.
FAU - Blatt, Sebastian
AU  - Blatt S
AD  - Department of Oral- and Maxillofacial Surgery, (Head: Univ.-Prof. Dr. Dr. B. 
      Al-Nawas), University Medical Center, Augustusplatz 2, 55131, Mainz, Germany.
FAU - Pabst, Andreas Max
AU  - Pabst AM
AD  - Department of Oral- and Maxillofacial Surgery, (Head: Prof. Dr. Dr. R. 
      Werkmeister), Federal Armed Forces Hospital, Rubenacherstrasse 170, 56072, 
      Koblenz, Germany.
FAU - Hansen, Torsten
AU  - Hansen T
AD  - Institute of Pathology, (Head: Prof. Dr. T. Hansen), Clinic Lippe, Rontgenstrasse 
      18, 32756, Detmold, Germany.
FAU - Goetz, Hermann
AU  - Goetz H
AD  - Biomaterials in Medicine (BioAPP), (Head: Univ.-Prof. Dr. Dr. B. Al-Nawas), 
      University Medical Center Mainz, Augustusplatz 2, 55131, Mainz, Germany.
FAU - Al-Nawas, Bilal
AU  - Al-Nawas B
AD  - Department of Oral- and Maxillofacial Surgery, (Head: Univ.-Prof. Dr. Dr. B. 
      Al-Nawas), University Medical Center, Augustusplatz 2, 55131, Mainz, Germany.
FAU - Ziebart, Thomas
AU  - Ziebart T
AD  - Department of Oral- and Maxillofacial Surgery, (Head: Univ.-Prof. Dr. Dr. A. 
      Neff), University Hospital Marburg, Baldingerstrasse, 35043, Marburg, Germany.
LA  - eng
PT  - Journal Article
DEP - 20180608
PL  - Scotland
TA  - J Craniomaxillofac Surg
JT  - Journal of cranio-maxillo-facial surgery : official publication of the European 
      Association for Cranio-Maxillo-Facial Surgery
JID - 8704309
RN  - 0 (Biocompatible Materials)
RN  - 0 (Bone Substitutes)
RN  - 0 (BoneCeramic)
RN  - 0 (Hydroxyapatites)
SB  - IM
MH  - Animals
MH  - Humans
MH  - Rats
MH  - Biocompatible Materials/pharmacology
MH  - *Bone Regeneration/physiology
MH  - *Bone Substitutes/pharmacology
MH  - Cone-Beam Computed Tomography
MH  - Disease Models, Animal
MH  - *Hydroxyapatites/pharmacology
MH  - *Neovascularization, Physiologic/physiology
MH  - Random Allocation
MH  - Rats, Nude
MH  - *Skull/diagnostic imaging/surgery
MH  - *Stem Cells/physiology
MH  - X-Ray Microtomography
OTO - NOTNLM
OT  - Angiogenesis
OT  - Bone regeneration
OT  - Bone substitute
OT  - EPC
OT  - Micro-CT
OT  - Vascularization
EDAT- 2018/09/11 06:00
MHDA- 2019/02/07 06:00
CRDT- 2018/09/11 06:00
PHST- 2017/12/25 00:00 [received]
PHST- 2018/05/23 00:00 [revised]
PHST- 2018/06/01 00:00 [accepted]
PHST- 2018/09/11 06:00 [entrez]
PHST- 2018/09/11 06:00 [pubmed]
PHST- 2019/02/07 06:00 [medline]
AID - S1010-5182(18)30387-1 [pii]
AID - 10.1016/j.jcms.2018.06.002 [doi]
PST - ppublish
SO  - J Craniomaxillofac Surg. 2018 Sep;46(9):1601-1608. doi: 
      10.1016/j.jcms.2018.06.002. Epub 2018 Jun 8.