PMID- 30197196
OWN - NLM
STAT- MEDLINE
DCOM- 20190611
LR  - 20190613
IS  - 1878-3279 (Electronic)
IS  - 0171-2985 (Linking)
VI  - 223
IP  - 12
DP  - 2018 Dec
TI  - Dectin-1 on macrophages modulates the immune response to Fasciola hepatica 
      products through the ERK signaling pathway.
PG  - 834-838
LID - S0171-2985(18)30082-2 [pii]
LID - 10.1016/j.imbio.2018.08.004 [doi]
AB  - Fasciolosis is a zoonotic disease of increasing importance due to its worldwide 
      distribution and elevated economic losses. Previously, we demonstrated that 
      Fasciola hepatica excretory-secretory products (FhESP) induce immunomodulatory 
      effects on peritoneal macrophages in a Dectin-1 dependent manner. In this study, 
      we observed that peritoneal macrophages from naive BALB/c mice stimulated in 
      vitro with FhESP presented increased expression levels of phosphorylated 
      extracellular-signal-regulated kinase (ERK), and this effect was dependent on 
      Syk, protein kinase C (PKC) and Dectin-1. In this sense, we observed increased 
      levels of arginase activity, IL-10 and TGF-beta in macrophages stimulated with 
      FhESP, which were dependent on PKC and ERK. Furthermore, we observed that the 
      increased arginase activity, as well as in TGF-beta and IL-10 levels, was partially 
      dependent on IL-10 receptor signaling in macrophages that were pre-incubated with 
      anti-IL10R before being stimulated with FhESP. Taken together, these results 
      suggest the participation of Dectin-1 and Syk in FhESP interaction with 
      peritoneal macrophages and the possible role of ERK and IL-10 in downstream 
      signaling pathways involved in the immunomodulatory effects induced by Fasciola 
      hepatica products.
CI  - Copyright (c) 2018 Elsevier GmbH. All rights reserved.
FAU - Guasconi, Lorena
AU  - Guasconi L
AD  - Departamento de Bioquimica Clinica, Facultad de Ciencias Quimicas, Universidad 
      Nacional de Cordoba, Centro de Investigaciones en Bioquimica Clinica e 
      Inmunologia (CIBICI - CONICET), Argentina. Electronic address: 
      lguasconi@fcq.unc.edu.ar.
FAU - Burstein, Veronica L
AU  - Burstein VL
AD  - Departamento de Bioquimica Clinica, Facultad de Ciencias Quimicas, Universidad 
      Nacional de Cordoba, Centro de Investigaciones en Bioquimica Clinica e 
      Inmunologia (CIBICI - CONICET), Argentina.
FAU - Beccacece, Ignacio
AU  - Beccacece I
AD  - Departamento de Bioquimica Clinica, Facultad de Ciencias Quimicas, Universidad 
      Nacional de Cordoba, Centro de Investigaciones en Bioquimica Clinica e 
      Inmunologia (CIBICI - CONICET), Argentina.
FAU - Mena, Cristian
AU  - Mena C
AD  - Departamento de Bioquimica Clinica, Facultad de Ciencias Quimicas, Universidad 
      Nacional de Cordoba, Centro de Investigaciones en Bioquimica Clinica e 
      Inmunologia (CIBICI - CONICET), Argentina.
FAU - Chiapello, Laura S
AU  - Chiapello LS
AD  - Departamento de Bioquimica Clinica, Facultad de Ciencias Quimicas, Universidad 
      Nacional de Cordoba, Centro de Investigaciones en Bioquimica Clinica e 
      Inmunologia (CIBICI - CONICET), Argentina.
FAU - Masih, Diana Teresa
AU  - Masih DT
AD  - Departamento de Bioquimica Clinica, Facultad de Ciencias Quimicas, Universidad 
      Nacional de Cordoba, Centro de Investigaciones en Bioquimica Clinica e 
      Inmunologia (CIBICI - CONICET), Argentina.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180810
PL  - Netherlands
TA  - Immunobiology
JT  - Immunobiology
JID - 8002742
RN  - 0 (Cytokines)
RN  - 0 (Lectins, C-Type)
RN  - 0 (dectin 1)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
RN  - EC 3.5.3.1 (Arginase)
SB  - IM
MH  - Animals
MH  - Arginase/metabolism
MH  - Cytokines/biosynthesis
MH  - Enzyme Activation/drug effects
MH  - Extracellular Signal-Regulated MAP Kinases/metabolism
MH  - Fasciola hepatica/*immunology
MH  - Fascioliasis/*immunology/metabolism/*parasitology
MH  - Female
MH  - *Immunomodulation
MH  - Lectins, C-Type/*metabolism
MH  - *MAP Kinase Signaling System
MH  - Macrophages/*immunology/*metabolism
MH  - Mice
MH  - Phosphorylation
OTO - NOTNLM
OT  - Dectin-1
OT  - Excretory-secretory products
OT  - Fasciola hepatica
OT  - Macrophage immunomodulation
EDAT- 2018/09/11 06:00
MHDA- 2019/06/14 06:00
CRDT- 2018/09/11 06:00
PHST- 2018/07/05 00:00 [received]
PHST- 2018/08/05 00:00 [revised]
PHST- 2018/08/08 00:00 [accepted]
PHST- 2018/09/11 06:00 [pubmed]
PHST- 2019/06/14 06:00 [medline]
PHST- 2018/09/11 06:00 [entrez]
AID - S0171-2985(18)30082-2 [pii]
AID - 10.1016/j.imbio.2018.08.004 [doi]
PST - ppublish
SO  - Immunobiology. 2018 Dec;223(12):834-838. doi: 10.1016/j.imbio.2018.08.004. Epub 
      2018 Aug 10.