PMID- 30197309
OWN - NLM
STAT- MEDLINE
DCOM- 20181115
LR  - 20211204
IS  - 1672-7347 (Print)
IS  - 1672-7347 (Linking)
VI  - 43
IP  - 8
DP  - 2018 Aug 28
TI  - [Alterations in gene expression profiles in children with Type 2 diabetes 
      mellitus and its mechanisms].
PG  - 826-834
LID - 10.11817/j.issn.1672-7347.2018.08.002 [doi]
AB  - To explore the mechanisms for Type 2 diabetes mellitus (T2DM) in children and 
      provide genomic evidence for its early diagnosis and treatment.
 Methods: The 
      peripheral blood gene chip datasets from 12 children with T2DM and 24 healthy 
      children were retrieved from the Gene Expression Omnibus (GEO) at National Center 
      for Biotechnology Information (NCBI). The differentially expressed genes were 
      screened by R language software. GenCLiP 2.0, STRING, and Cytoscape software were 
      used to analyze the biological functions, protein-protein interaction network, 
      signal pathway, gene-pathway network, expression of key genes, and predictive 
      value between the two differentially expressed genes.
 Results: A total of 79 
      differentially expressed genes were identified. Among them, 58 (73.42%) were 
      up-regulated, and 21 (26.58%) were down-regulated. Differentially expressed genes 
      mainly involved molecular functions and biological processes, such as defensive 
      response, response to external stimulus, and inflammatory responses. At the same 
      time, they were mainly involved in the Leishmaniasis, cytokine-cytokine receptor 
      interaction, Toll-like receptor signaling pathway. interleukin 1beta (IL-1beta), jun 
      proto-oncogene (JUN), and IL-8 were 3 important linking nodes in the 
      protein-protein interaction network. JUN and IL-1beta were key genes, which were 
      related to interleukin 17 (1L-17) signaling pathway, Toll-like receptor signaling 
      pathway and so on. The expression of JUN gene in peripheral blood of children 
      with T2DM was decreased while the expression of IL-1beta gene was increased. JUN and 
      IL-1beta genes possessed certain diagnostic and predictive value in children with 
      T2DM.
 Conclusion: The gene expression profile of peripheral blood in children 
      with T2DM changes significantly. The genes of JUN and IL-1beta are closely related 
      to T2DM in children. IL-1beta gene expression level shows a better predictive value 
      on T2DM in children.
FAU - Jia, Lili
AU  - Jia L
AD  - Department of Health Management, School of Humanities and Management, Shaanxi 
      University of Chinese Medicine, Xianyang Shaanxi 712046, China.
FAU - Zhang, Rongqiang
AU  - Zhang R
AD  - Department of Epidemiology and Health Statistics, School of Public Health, 
      Shaanxi University of Chinese Medicine, Xianyang Shaanxi 712046, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhong Nan Da Xue Xue Bao Yi Xue Ban
JT  - Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. 
      Medical sciences
JID - 101230586
RN  - 0 (Interleukin-1beta)
RN  - 0 (MAS1 protein, human)
RN  - 0 (Proto-Oncogene Mas)
RN  - 0 (Proto-Oncogene Proteins c-jun)
SB  - IM
MH  - Child
MH  - Diabetes Mellitus, Type 2/diagnosis/*genetics/therapy
MH  - Down-Regulation
MH  - *Gene Expression Profiling
MH  - Humans
MH  - Interleukin-1beta/*genetics
MH  - Oligonucleotide Array Sequence Analysis
MH  - Proto-Oncogene Mas
MH  - Proto-Oncogene Proteins c-jun/*genetics
MH  - Signal Transduction/genetics
MH  - Software
MH  - *Transcriptome
MH  - Up-Regulation
EDAT- 2018/09/11 06:00
MHDA- 2018/11/16 06:00
CRDT- 2018/09/11 06:00
PHST- 2018/09/11 06:00 [entrez]
PHST- 2018/09/11 06:00 [pubmed]
PHST- 2018/11/16 06:00 [medline]
AID - 10.11817/j.issn.1672-7347.2018.08.002 [doi]
PST - ppublish
SO  - Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2018 Aug 28;43(8):826-834. doi: 
      10.11817/j.issn.1672-7347.2018.08.002.