PMID- 30197645
OWN - NLM
STAT- MEDLINE
DCOM- 20190919
LR  - 20230928
IS  - 1664-3224 (Electronic)
IS  - 1664-3224 (Linking)
VI  - 9
DP  - 2018
TI  - Establishment and Characterization of a Functionally Competent Type 2 
      Conventional Dendritic Cell Line.
PG  - 1912
LID - 10.3389/fimmu.2018.01912 [doi]
LID - 1912
AB  - Dendritic cells (DCs) are the most potent antigen presenting cells and possess an 
      incomparable ability to activate and instruct T cells, which makes them one of 
      the cornerstones in the regulation of the cross-talk between innate and adaptive 
      immunity. Therefore, a deep understanding of DC biology lays the foundations to 
      describe and to harness the mechanisms that regulate the development of the 
      adaptive response, with clear implications in a vast array of fields such as the 
      study of autoimmune diseases and the development of new vaccines. However, the 
      great difficulty to obtain large quantities of viable non-activated DCs for 
      experimentation have considerably hindered the progress of DC research. Several 
      strategies have been proposed to overcome these limitations by promoting an 
      increase of DC abundance in vivo, by inducing DC development from DC progenitors 
      in vitro and by generating stable DC lines. In the past years, we have described 
      a method to derive immortalized stable DC lines, named MutuDCs, from the spleens 
      of Mushi1 mice, a transgenic mouse strain that express the simian virus 40 Large 
      T-oncogene in the DCs. The comparison of these DC lines with the vast variety of 
      DC subsets described in vivo has shown that all the MutuDC lines that we have 
      generated so far have phenotypic and functional features of type 1 conventional 
      DCs (cDC1s). With the purpose of deriving DC lines with characteristics of type 2 
      conventional DCs (cDC2s), we bred a new Batf3(-/-) Mushi1 murine line in which 
      the development of the cDC1 subset is severely defective. The new MutuDC line 
      that we generated from Batf3(-/-) Mushi1 mice was phenotypically and functionally 
      characterized in this work. Our results demonstrated that all the tested 
      characteristics of this new cell line, including the expression of 
      subset-determining transcription factors, the profile of cytokine production and 
      the ability to present antigens, are comparable with the features of splenic 
      CD4(-) cDC2s. Therefore, we concluded that our new cell line, that we named 
      CD4(-) MutuDC2 line, represents a valuable model for the CD4(-) cDC2 subset.
FAU - Pigni, Matteo
AU  - Pigni M
AD  - Department of Biochemistry CIIL, University of Lausanne, Epalinges, Switzerland.
FAU - Ashok, Devika
AU  - Ashok D
AD  - Department of Biochemistry CIIL, University of Lausanne, Epalinges, Switzerland.
FAU - Stevanin, Mathias
AU  - Stevanin M
AD  - Department of Biochemistry CIIL, University of Lausanne, Epalinges, Switzerland.
FAU - Acha-Orbea, Hans
AU  - Acha-Orbea H
AD  - Department of Biochemistry CIIL, University of Lausanne, Epalinges, Switzerland.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180824
PL  - Switzerland
TA  - Front Immunol
JT  - Frontiers in immunology
JID - 101560960
SB  - IM
MH  - *Adaptive Immunity
MH  - Animals
MH  - *Cell Line
MH  - Dendritic Cells/*cytology/*immunology
MH  - Mice
MH  - Mice, Knockout
MH  - Spleen/cytology/immunology
PMC - PMC6117413
OTO - NOTNLM
OT  - cDC1
OT  - cDC2
OT  - cell culture
OT  - cell line
OT  - conventional DC subset
OT  - dendritic cell
OT  - mouse
OT  - spleen
EDAT- 2018/09/11 06:00
MHDA- 2019/09/20 06:00
PMCR- 2018/01/01
CRDT- 2018/09/11 06:00
PHST- 2018/05/18 00:00 [received]
PHST- 2018/08/02 00:00 [accepted]
PHST- 2018/09/11 06:00 [entrez]
PHST- 2018/09/11 06:00 [pubmed]
PHST- 2019/09/20 06:00 [medline]
PHST- 2018/01/01 00:00 [pmc-release]
AID - 10.3389/fimmu.2018.01912 [doi]
PST - epublish
SO  - Front Immunol. 2018 Aug 24;9:1912. doi: 10.3389/fimmu.2018.01912. eCollection 
      2018.