PMID- 3019991
OWN - NLM
STAT- MEDLINE
DCOM- 19861030
LR  - 20191022
IS  - 0883-8364 (Print)
IS  - 0883-8364 (Linking)
VI  - 22
IP  - 9
DP  - 1986 Sep
TI  - Increased yield of mouse mammary tumor virus (MMTV) by cultivation of 
      monolayer-derived mammary tumor cells in suspension.
PG  - 542-8
AB  - The MJY-alpha epithelial-like mammary tumor cell line was adapted for cultivation 
      in suspension using a shaker culture technique. Replication of suspension 
      (MJY-beta) cells was more sensitive than monolayer cells to decreases in the 
      concentration of serum in the medium. Comparison of amino acid incorporation and 
      lactate production rates revealed additional differences between monolayer and 
      suspension cultures. In addition, growth in suspension resulted in 10- to 
      400-fold increases in mouse mammary tumor virus (MMTV) production by the mammary 
      tumor cells. Increases in MMTV yield were detected within 48 h of culture 
      initiation and MMTV production remained elevated throughout 20 cell passages in 
      suspension. Exposure of MJY-beta cells to 14 microM hydrocortisone further 
      increased MMTV yield two- to five-fold. The MJY-beta suspension cultures 
      demonstrated that these epithelial-like cells do not require attachment to a 
      solid substrate for replication or for MMTV production. Loss of structural 
      polarization associated with growth as a monolayer resulted in stimulation of 
      MMTV production greater than and independent of steroid exposure.
FAU - Maldarelli, F
AU  - Maldarelli F
FAU - Yagi, M J
AU  - Yagi MJ
LA  - eng
GR  - 5P-30CA23102/CA/NCI NIH HHS/United States
GR  - GM07280/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - In Vitro Cell Dev Biol
JT  - In vitro cellular & developmental biology : journal of the Tissue Culture 
      Association
JID - 8506951
RN  - 0 (DNA, Neoplasm)
RN  - 0 (Lactates)
RN  - 0 (RNA, Neoplasm)
SB  - IM
MH  - Animals
MH  - Cell Adhesion
MH  - Cell Division
MH  - Cells, Cultured
MH  - DNA, Neoplasm/biosynthesis
MH  - Epithelium/pathology
MH  - Lactates/metabolism
MH  - Mammary Neoplasms, Experimental/*microbiology/pathology
MH  - Mammary Tumor Virus, Mouse/*growth & development
MH  - Mice
MH  - RNA, Neoplasm/biosynthesis
MH  - Virus Replication
EDAT- 1986/09/01 00:00
MHDA- 1986/09/01 00:01
CRDT- 1986/09/01 00:00
PHST- 1986/09/01 00:00 [pubmed]
PHST- 1986/09/01 00:01 [medline]
PHST- 1986/09/01 00:00 [entrez]
AID - 10.1007/BF02621141 [doi]
PST - ppublish
SO  - In Vitro Cell Dev Biol. 1986 Sep;22(9):542-8. doi: 10.1007/BF02621141.