PMID- 30201095
OWN - NLM
STAT- MEDLINE
DCOM- 20190326
LR  - 20191204
IS  - 1097-4164 (Electronic)
IS  - 1097-2765 (Print)
IS  - 1097-2765 (Linking)
VI  - 71
IP  - 5
DP  - 2018 Sep 6
TI  - Lamins Organize the Global Three-Dimensional Genome from the Nuclear Periphery.
PG  - 802-815.e7
LID - S1097-2765(18)30391-5 [pii]
LID - 10.1016/j.molcel.2018.05.017 [doi]
AB  - Lamins are structural components of the nuclear lamina (NL) that regulate genome 
      organization and gene expression, but the mechanism remains unclear. Using Hi-C, 
      we show that lamins maintain proper interactions among the topologically 
      associated chromatin domains (TADs) but not their overall architecture. Combining 
      Hi-C with fluorescence in situ hybridization (FISH) and analyses of 
      lamina-associated domains (LADs), we reveal that lamin loss causes expansion or 
      detachment of specific LADs in mouse ESCs. The detached LADs disrupt 3D 
      interactions of both LADs and interior chromatin. 4C and epigenome analyses 
      further demonstrate that lamins maintain the active and repressive chromatin 
      domains among different TADs. By combining these studies with transcriptome 
      analyses, we found a significant correlation between transcription changes and 
      the interaction changes of active and inactive chromatin domains These findings 
      provide a foundation to further study how the nuclear periphery impacts genome 
      organization and transcription in development and NL-associated diseases.
CI  - Copyright (c) 2018 Elsevier Inc. All rights reserved.
FAU - Zheng, Xiaobin
AU  - Zheng X
AD  - Department of Embryology, Carnegie Institution for Science, Baltimore, MD 21218, 
      USA. Electronic address: xzheng@carnegiescience.edu.
FAU - Hu, Jiabiao
AU  - Hu J
AD  - Department of Embryology, Carnegie Institution for Science, Baltimore, MD 21218, 
      USA.
FAU - Yue, Sibiao
AU  - Yue S
AD  - Department of Embryology, Carnegie Institution for Science, Baltimore, MD 21218, 
      USA.
FAU - Kristiani, Lidya
AU  - Kristiani L
AD  - Soonchunhyang Institute of Medi-Bio Science (SIMS), Soonchunhyang University, 25 
      Bongjeong-ro, Cheonan-si, Chungcheongnam-do 31151, Korea.
FAU - Kim, Miri
AU  - Kim M
AD  - Soonchunhyang Institute of Medi-Bio Science (SIMS), Soonchunhyang University, 25 
      Bongjeong-ro, Cheonan-si, Chungcheongnam-do 31151, Korea.
FAU - Sauria, Michael
AU  - Sauria M
AD  - Department of Biology and Department of Computer Science, The Johns Hopkins 
      University, Baltimore, MD 21218, USA.
FAU - Taylor, James
AU  - Taylor J
AD  - Department of Biology and Department of Computer Science, The Johns Hopkins 
      University, Baltimore, MD 21218, USA.
FAU - Kim, Youngjo
AU  - Kim Y
AD  - Soonchunhyang Institute of Medi-Bio Science (SIMS), Soonchunhyang University, 25 
      Bongjeong-ro, Cheonan-si, Chungcheongnam-do 31151, Korea. Electronic address: 
      yjokim@sch.ac.kr.
FAU - Zheng, Yixian
AU  - Zheng Y
AD  - Department of Embryology, Carnegie Institution for Science, Baltimore, MD 21218, 
      USA. Electronic address: zheng@carnegiescience.edu.
LA  - eng
GR  - R01 GM056312/GM/NIGMS NIH HHS/United States
GR  - R01 GM106023/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20180628
PL  - United States
TA  - Mol Cell
JT  - Molecular cell
JID - 9802571
RN  - 0 (Chromatin)
RN  - 0 (Lamins)
SB  - IM
MH  - Animals
MH  - Cell Nucleus/*genetics
MH  - Chromatin/genetics
MH  - Chromatin Assembly and Disassembly/genetics
MH  - Epigenomics/methods
MH  - Gene Expression/genetics
MH  - Genome/*genetics
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Lamins/*genetics
MH  - Mice
MH  - Nuclear Lamina/*genetics
PMC - PMC6886264
MID - NIHMS969347
OTO - NOTNLM
OT  - 3D genome
OT  - Hi-C
OT  - HiLands
OT  - LADs
OT  - TADs
OT  - histone and lamina landscape
OT  - lamin
OT  - lamina-associated chromatin domains
OT  - nuclear lamina
OT  - transcription
COIS- Declaration of Interests The authors declare no competing interests.
EDAT- 2018/09/12 06:00
MHDA- 2019/03/27 06:00
PMCR- 2019/12/02
CRDT- 2018/09/12 06:00
PHST- 2017/06/04 00:00 [received]
PHST- 2018/04/01 00:00 [revised]
PHST- 2018/05/15 00:00 [accepted]
PHST- 2018/09/12 06:00 [entrez]
PHST- 2018/09/12 06:00 [pubmed]
PHST- 2019/03/27 06:00 [medline]
PHST- 2019/12/02 00:00 [pmc-release]
AID - S1097-2765(18)30391-5 [pii]
AID - 10.1016/j.molcel.2018.05.017 [doi]
PST - ppublish
SO  - Mol Cell. 2018 Sep 6;71(5):802-815.e7. doi: 10.1016/j.molcel.2018.05.017. Epub 
      2018 Jun 28.