PMID- 30201873 OWN - NLM STAT- MEDLINE DCOM- 20181112 LR - 20201209 IS - 1999-4915 (Electronic) IS - 1999-4915 (Linking) VI - 10 IP - 9 DP - 2018 Sep 10 TI - Evolution of Tembusu Virus in Ducks, Chickens, Geese, Sparrows, and Mosquitoes in Northern China. LID - 10.3390/v10090485 [doi] LID - 485 AB - Tembusu virus (TMUV) is a contagious pathogen from fowl that mainly infects ducks and geese, causing symptoms of high fever, loss of appetite, retarded growth, neurological symptoms, severe duck-drop syndrome, and even death. During an epidemiological investigation of TMUV in Northern China, we isolated 11 TMUV strains from ducks, chickens, geese, sparrows, and mosquitoes (2011(-)2017). Phylogenetic analysis of the open-reading frames of genes revealed that these strains clustered into Chinese strains II. The nucleotide and amino acid homologies of NS1 of the strains ranged between 85.8(-)99.8% and 92.5(-)99.68%, respectively, which were lower than those of E (86.7(-)99.9% and 96.5(-)99.9%, respectively), NS3 (87.6(-)99.9% and 98.2(-)99.8%, respectively), and NS5 (86.5(-)99.9% and 97.8(-)99.9%, respectively). Predictions of the tertiary structure of the viral proteins indicated that NS1 in 4 of 11 strains had a protein structure mutation at (180)TAV(182) that changed a random crimp into an alpha helix. The protein of 6 of 11 strains had a glycosylation site mutation from NTTD to NITD. Furthermore, epidemiological data suggested that TMUV has been circulating in half of China's provinces (17 of 34). Our findings, for the first time, have identified the NS1 protein as a potential hypervariable region for genetic evolution. Additionally, the territorial scope of the virus has expanded, requiring strict bio-security measures or a multivalent vaccine to control its spread. FAU - Yu, Guanliu AU - Yu G AUID- ORCID: 0000-0003-2928-0299 AD - College of Animal Science and Technology, Shandong Agricultural University, 61 Daizong Road, Tai'an 271018, Shandong Province, China. yuguanliu@163.com. AD - Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, 61 Daizong Road, Tai'an 271018, Shandong Province, China. yuguanliu@163.com. AD - Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Shandong Agricultural University, 61 Daizong Road, Tai'an 271018, Shandong Province, China. yuguanliu@163.com. FAU - Lin, Yun AU - Lin Y AD - College of Animal Science and Technology, Shandong Agricultural University, 61 Daizong Road, Tai'an 271018, Shandong Province, China. lyun1994@126.com. AD - Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, 61 Daizong Road, Tai'an 271018, Shandong Province, China. lyun1994@126.com. AD - Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Shandong Agricultural University, 61 Daizong Road, Tai'an 271018, Shandong Province, China. lyun1994@126.com. FAU - Tang, Yi AU - Tang Y AD - College of Animal Science and Technology, Shandong Agricultural University, 61 Daizong Road, Tai'an 271018, Shandong Province, China. tyck288@163.com. AD - Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, 61 Daizong Road, Tai'an 271018, Shandong Province, China. tyck288@163.com. AD - Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Shandong Agricultural University, 61 Daizong Road, Tai'an 271018, Shandong Province, China. tyck288@163.com. FAU - Diao, Youxiang AU - Diao Y AD - College of Animal Science and Technology, Shandong Agricultural University, 61 Daizong Road, Tai'an 271018, Shandong Province, China. yxdiao@126.com. AD - Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, 61 Daizong Road, Tai'an 271018, Shandong Province, China. yxdiao@126.com. AD - Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Shandong Agricultural University, 61 Daizong Road, Tai'an 271018, Shandong Province, China. yxdiao@126.com. LA - eng GR - 31472199/National Natural Science Foundation of China/International GR - 2016YFD0500107/National Key Research and Development Program of China/International GR - CARS-42-19/China Agriculture Research System/International GR - 2014GNC111023/Science and Technology Development Plan of Shandong Province/International GR - Shandong "Double Tops" Program/Funds of Shandong "Double Tops" Program/International PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20180910 PL - Switzerland TA - Viruses JT - Viruses JID - 101509722 RN - 0 (Viral Proteins) RN - Tembusu virus SB - IM MH - Animals MH - Bird Diseases/epidemiology/*virology MH - Birds/*virology MH - China/epidemiology MH - Disease Outbreaks MH - Evolution, Molecular MH - Flavivirus/*classification/genetics/*isolation & purification MH - Flavivirus Infections/epidemiology/*veterinary/virology MH - *Genetic Variation MH - Mosquito Vectors/*virology MH - Mutation, Missense MH - Phylogeny MH - Protein Conformation MH - Sequence Homology, Amino Acid MH - Sequence Homology, Nucleic Acid MH - Viral Proteins/chemistry/genetics PMC - PMC6164154 OTO - NOTNLM OT - Tembusu virus OT - duck OT - glycosylation OT - phylogenetic analysis OT - protein structure COIS- The authors have no conflicts of interest to declare. EDAT- 2018/09/12 06:00 MHDA- 2018/11/13 06:00 PMCR- 2018/09/01 CRDT- 2018/09/12 06:00 PHST- 2018/08/21 00:00 [received] PHST- 2018/09/05 00:00 [revised] PHST- 2018/09/06 00:00 [accepted] PHST- 2018/09/12 06:00 [entrez] PHST- 2018/09/12 06:00 [pubmed] PHST- 2018/11/13 06:00 [medline] PHST- 2018/09/01 00:00 [pmc-release] AID - v10090485 [pii] AID - viruses-10-00485 [pii] AID - 10.3390/v10090485 [doi] PST - epublish SO - Viruses. 2018 Sep 10;10(9):485. doi: 10.3390/v10090485.