PMID- 30202442 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20220321 IS - 1755-8166 (Print) IS - 1755-8166 (Electronic) IS - 1755-8166 (Linking) VI - 11 DP - 2018 TI - Chromosomes of Asian cyprinid fishes: cytogenetic analysis of two representatives of small paleotetraploid tribe Probarbini. PG - 51 LID - 10.1186/s13039-018-0399-8 [doi] LID - 51 AB - BACKGROUND: Polyploidy, although still poorly explored, represents an important evolutionary event in several cyprinid clades. Herein, Catlocarpio siamensis and Probarbus jullieni - representatives of the paleotetraploid tribe Probarbini, were characterized both by conventional and molecular cytogenetic methods. RESULTS: Alike most other paleotetraploid cyprinids (with 2n = 100), both species studied here shared 2n = 98 but differed in karyotypes: C. siamensis displayed 18m + 34sm + 46st/a; NF = 150, while P. jullieni exhibited 26m + 14sm + 58st/a; NF = 138. Fluorescence in situ hybridization (FISH) with rDNA probes revealed two (5S) and eight (18S) signals in C. siamensis, respectively, and six signals for both probes in P. jullieni. FISH with microsatellite motifs evidenced substantial genomic divergence between both species. The almost doubled size of the chromosome pairs #1 in C. siamensis and #14 in P. jullieni compared to the rest of corresponding karyotypes indicated chromosomal fusions. CONCLUSION: Based on our findings, together with likely the same reduced 2n = 98 karyotypes in the remainder Probarbini species, we hypothesize that the karyotype 2n = 98 might represent a derived character, shared by all members of the Probarbini clade. Besides, we also witnessed considerable changes in the amount and distribution of certain repetitive DNA classes, suggesting complex post-polyploidization processes in this small paleotetraploid tribe. FAU - Saenjundaeng, Pasakorn AU - Saenjundaeng P AD - 1Toxic Substances in Livestock and Aquatic Animals Research Group, Department of Biology, Faculty of Science, Khon Kaen University, Muang District, Khon Kaen, Thailand. ISNI: 0000 0004 0470 0856. GRID: grid.9786.0 FAU - de Bello Cioffi, Marcelo AU - de Bello Cioffi M AD - 2Departamento de Genetica e Evolucao, Universidade Federal de Sao Carlos, Sao Carlos, SP Brazil. ISNI: 0000 0001 2163 588X. GRID: grid.411247.5 FAU - de Oliveira, Ezequiel Aguiar AU - de Oliveira EA AD - 2Departamento de Genetica e Evolucao, Universidade Federal de Sao Carlos, Sao Carlos, SP Brazil. ISNI: 0000 0001 2163 588X. GRID: grid.411247.5 AD - Secretaria de Estado de Educacao de Mato Grosso - SEDUC-MT, Cuiaba, MT Brazil. FAU - Tanomtong, Alongklod AU - Tanomtong A AD - 1Toxic Substances in Livestock and Aquatic Animals Research Group, Department of Biology, Faculty of Science, Khon Kaen University, Muang District, Khon Kaen, Thailand. ISNI: 0000 0004 0470 0856. GRID: grid.9786.0 FAU - Supiwong, Weerayuth AU - Supiwong W AD - 4Faculty of Applied Science and Engineering, Khon Kaen University, Nong Kai Campus, Muang, Nong Kai Thailand. ISNI: 0000 0004 0470 0856. GRID: grid.9786.0 FAU - Phimphan, Sumalee AU - Phimphan S AD - 1Toxic Substances in Livestock and Aquatic Animals Research Group, Department of Biology, Faculty of Science, Khon Kaen University, Muang District, Khon Kaen, Thailand. ISNI: 0000 0004 0470 0856. GRID: grid.9786.0 FAU - Collares-Pereira, Maria Joao AU - Collares-Pereira MJ AD - 5Faculdade de Ciencias, Centre for Ecology, Evolution and Environmental Changes, Universidade de Lisboa, Campo Grande, PT-1749-016 Lisbon, Portugal. ISNI: 0000 0001 2181 4263. GRID: grid.9983.b FAU - Sember, Alexandr AU - Sember A AD - 6Laboratory of Fish Genetics, Institute of Animal Physiology and Genetics, Czech Academy of Sciences, Rumburska 89, 277 21 Libechov, Czech Republic. ISNI: 0000 0001 1015 3316. GRID: grid.418095.1 FAU - Bertollo, Luiz Antonio Carlos AU - Bertollo LAC AD - 2Departamento de Genetica e Evolucao, Universidade Federal de Sao Carlos, Sao Carlos, SP Brazil. ISNI: 0000 0001 2163 588X. GRID: grid.411247.5 FAU - Liehr, Thomas AU - Liehr T AD - 7Institute of Human Genetics, Jena University Hospital, Am Klinikum 1, D-07747 Jena, Germany. ISNI: 0000 0000 8517 6224. GRID: grid.275559.9 FAU - Yano, Cassia Fernanda AU - Yano CF AD - 2Departamento de Genetica e Evolucao, Universidade Federal de Sao Carlos, Sao Carlos, SP Brazil. ISNI: 0000 0001 2163 588X. GRID: grid.411247.5 FAU - Hatanaka, Terumi AU - Hatanaka T AD - 2Departamento de Genetica e Evolucao, Universidade Federal de Sao Carlos, Sao Carlos, SP Brazil. ISNI: 0000 0001 2163 588X. GRID: grid.411247.5 FAU - Rab, Petr AU - Rab P AD - 6Laboratory of Fish Genetics, Institute of Animal Physiology and Genetics, Czech Academy of Sciences, Rumburska 89, 277 21 Libechov, Czech Republic. ISNI: 0000 0001 1015 3316. GRID: grid.418095.1 LA - eng PT - Journal Article DEP - 20180904 PL - England TA - Mol Cytogenet JT - Molecular cytogenetics JID - 101317942 PMC - PMC6123905 OTO - NOTNLM OT - Chromosomal markers OT - Cyprinidae OT - Fish cytogenetics OT - Microsatellites OT - rDNAs COIS- Experiments were performed in accordance with ethical protocols, and anesthesia using clove oil was administered prior to sacrificing the animals, as approved by the Ethics Committee of Khon Kaen University and by the RGJ committee under no. PHD/K0081/2556.Not applicableThe authors declare that they have no competing interests.Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. EDAT- 2018/09/12 06:00 MHDA- 2018/09/12 06:01 PMCR- 2018/09/04 CRDT- 2018/09/12 06:00 PHST- 2018/07/16 00:00 [received] PHST- 2018/08/23 00:00 [accepted] PHST- 2018/09/12 06:00 [entrez] PHST- 2018/09/12 06:00 [pubmed] PHST- 2018/09/12 06:01 [medline] PHST- 2018/09/04 00:00 [pmc-release] AID - 399 [pii] AID - 10.1186/s13039-018-0399-8 [doi] PST - epublish SO - Mol Cytogenet. 2018 Sep 4;11:51. doi: 10.1186/s13039-018-0399-8. eCollection 2018.