PMID- 30209641
OWN - NLM
STAT- MEDLINE
DCOM- 20190401
LR  - 20190401
IS  - 1436-5073 (Electronic)
IS  - 0026-3672 (Linking)
VI  - 185
IP  - 10
DP  - 2018 Sep 12
TI  - Determination of hypoxia-inducible factor-1 by using a ratiometric colorimetric 
      test based on click-mediated growth of gold nanoparticles.
PG  - 451
LID - 10.1007/s00604-018-2992-2 [doi]
AB  - The authors describe a significantly improved colorimetric nanoprobe for the 
      determination of transcription factors (TFs). It is making use of click-mediated 
      growth of gold nanoparticles (AuNPs) to amplify the signal-to-noise ratio. 
      Hypoxia-inducible factor-1 (HIF-1) is an important TF that acts as a mediator of 
      cell response to hypoxia. So, the detection of HIF-1 was chosen as the model 
      analyte. Specifically, target HIF-1 is designed to bind to the hypoxia response 
      element within DNA duplex. The click chemistry between the DNA duplex and 
      alkynyl-functionalized AuNPs (AF-AuNPs) is then inhibited because of significant 
      steric hindrance. As a result, the AF-AuNPs grow into larger-sized 
      highly-aggregated irregular nanostructures, which in turn enable colorimetric 
      determination. The ratio of absorbances at 620 and 560 nm increases in the 0.5 to 
      10 nM HIF-1 concentration range, and the detection limit is 0.27 nM. This is 
      better by a factor of 100 than that of aggregation-based colorimetric assays. The 
      nanoprobe is selective and can be used in complex samples. Conceivably, it may 
      also be extended to the determination of other TFs by simply changing the used 
      DNA duplex. Graphical abstract Schematic of a nanoprobe for detecting 
      hypoxia-inducible factor-1 (HIF-1). Three concepts are involved: the binding of 
      HIF-1 and hypoxia response element, the Cu(+)-catalyzed click chemistry between 
      P1/P2 duplex and alkynyl-functionalized AuNPs (AF-AuNPs), and the AuNPs growth 
      with hydroxylamine and HAuCl(4).
FAU - Chen, Hong
AU  - Chen H
AD  - Center for Molecular Recognition and Biosensing, School of Life Sciences, 
      Shanghai University, Shanghai, 200444, China.
FAU - Sun, Yan
AU  - Sun Y
AD  - Department of Endocrinology, Xuzhou Central Hospital, The Affiliated XuZhou 
      Hospital of Medical College of Southeast University, Xuzhou, 221009, China.
FAU - Li, Yifei
AU  - Li Y
AD  - Center for Molecular Recognition and Biosensing, School of Life Sciences, 
      Shanghai University, Shanghai, 200444, China.
FAU - Zhao, Jing
AU  - Zhao J
AD  - Center for Molecular Recognition and Biosensing, School of Life Sciences, 
      Shanghai University, Shanghai, 200444, China. jingzhao@t.shu.edu.cn.
AD  - Shanghai Key Laboratory of Bio-Energy Crops, School of Life Sciences, Shanghai 
      University, Shanghai, 200444, People's Republic of China. jingzhao@t.shu.edu.cn.
FAU - Cao, Ya
AU  - Cao Y
AD  - Center for Molecular Recognition and Biosensing, School of Life Sciences, 
      Shanghai University, Shanghai, 200444, China. conezimint@shu.edu.cn.
LA  - eng
GR  - 81671781/National Natural Science Foundation of China/International
GR  - 81401489/National Natural Science Foundation of China/International
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180912
PL  - Austria
TA  - Mikrochim Acta
JT  - Mikrochimica acta
JID - 7808782
RN  - 0 (Hypoxia-Inducible Factor 1)
RN  - 7440-57-5 (Gold)
SB  - IM
MH  - Click Chemistry
MH  - Colorimetry/*methods
MH  - Gold/*chemistry
MH  - Humans
MH  - Hypoxia-Inducible Factor 1/*analysis
MH  - Limit of Detection
MH  - Metal Nanoparticles/*chemistry
OTO - NOTNLM
OT  - Alkynyl-functionalized gold nanoparticle
OT  - Click chemistry
OT  - Cu+-alkynyl complex
OT  - Estrogen receptor alpha
OT  - Gold nanostructure
OT  - Hypoxia response element
OT  - Seed-mediated growth
OT  - Transcription factor
EDAT- 2018/09/14 06:00
MHDA- 2019/04/02 06:00
CRDT- 2018/09/14 06:00
PHST- 2018/07/08 00:00 [received]
PHST- 2018/09/01 00:00 [accepted]
PHST- 2018/09/14 06:00 [entrez]
PHST- 2018/09/14 06:00 [pubmed]
PHST- 2019/04/02 06:00 [medline]
AID - 10.1007/s00604-018-2992-2 [pii]
AID - 10.1007/s00604-018-2992-2 [doi]
PST - epublish
SO  - Mikrochim Acta. 2018 Sep 12;185(10):451. doi: 10.1007/s00604-018-2992-2.