PMID- 30209717
OWN - NLM
STAT- MEDLINE
DCOM- 20200721
LR  - 20231213
IS  - 1573-7039 (Electronic)
IS  - 1083-3021 (Linking)
VI  - 24
IP  - 1
DP  - 2019 Mar
TI  - A Transgenic MMTV-Flippase Mouse Line for Molecular Engineering in Mammary Gland 
      and Breast Cancer Mouse Models.
PG  - 39-45
LID - 10.1007/s10911-018-9412-4 [doi]
AB  - Genetically engineered mouse models have become an indispensable tool for breast 
      cancer research. Combination of multiple site-specific recombination systems such 
      as Cre/loxP and Flippase (Flp)/Frt allows for engineering of sophisticated, 
      multi-layered conditional mouse models. Here, we report the generation and 
      characterization of a novel transgenic mouse line expressing a mouse 
      codon-optimized Flp under the control of the mouse mammary tumor virus (MMTV) 
      promoter. These mice show robust Flp-mediated recombination in luminal mammary 
      gland and breast cancer cells but no Flp activity in non-mammary tissues, with 
      the exception of limited activity in salivary glands. These mice provide a unique 
      tool for studying mammary gland biology and carcinogenesis in mice.
FAU - Luond, Fabiana
AU  - Luond F
AD  - Department of Biomedicine, University of Basel, Mattenstrasse 28, 4058, Basel, 
      Switzerland.
FAU - Bill, Ruben
AU  - Bill R
AD  - Department of Biomedicine, University of Basel, Mattenstrasse 28, 4058, Basel, 
      Switzerland.
FAU - Vettiger, Andrea
AU  - Vettiger A
AD  - Department of Biomedicine, University of Basel, Mattenstrasse 28, 4058, Basel, 
      Switzerland.
AD  - Focal Area Infection Biology, Biozentrum University of Basel, Klingelbergstrasse 
      50/70, 4056, Basel, Switzerland.
FAU - Oller, Heide
AU  - Oller H
AD  - Center for Transgenic Models, University of Basel, Mattenstrasse 22, 4058, Basel, 
      Switzerland.
FAU - Pelczar, Pawel
AU  - Pelczar P
AD  - Center for Transgenic Models, University of Basel, Mattenstrasse 22, 4058, Basel, 
      Switzerland.
FAU - Christofori, Gerhard
AU  - Christofori G
AD  - Department of Biomedicine, University of Basel, Mattenstrasse 28, 4058, Basel, 
      Switzerland. gerhard.christofori@unibas.ch.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180912
PL  - United States
TA  - J Mammary Gland Biol Neoplasia
JT  - Journal of mammary gland biology and neoplasia
JID - 9601804
RN  - 0 (Luminescent Proteins)
RN  - EC 2.7.7.- (DNA Nucleotidyltransferases)
RN  - EC 2.7.7.- (FLP recombinase)
SB  - IM
MH  - Animals
MH  - Carcinogenesis/*genetics/pathology
MH  - DNA Nucleotidyltransferases/*genetics
MH  - Disease Progression
MH  - Epithelial Cells/pathology
MH  - Female
MH  - Genes, Reporter/genetics
MH  - Genetic Vectors/administration & dosage/genetics
MH  - Luminescent Proteins/genetics
MH  - Mammary Glands, Animal/cytology/*pathology
MH  - Mammary Neoplasms, Experimental/*genetics/pathology
MH  - Mammary Tumor Virus, Mouse/*genetics
MH  - Mice
MH  - Mice, Transgenic
MH  - Microinjections
MH  - Promoter Regions, Genetic/genetics
MH  - Recombination, Genetic
MH  - Salivary Glands/pathology
MH  - Tumor Microenvironment/genetics
MH  - Red Fluorescent Protein
OTO - NOTNLM
OT  - Breast cancer
OT  - Flippase
OT  - Lineage tracing
OT  - MMTV promoter
OT  - Mammary gland
OT  - Transgenic mice
EDAT- 2018/09/14 06:00
MHDA- 2020/07/22 06:00
CRDT- 2018/09/14 06:00
PHST- 2018/06/06 00:00 [received]
PHST- 2018/09/04 00:00 [accepted]
PHST- 2018/09/14 06:00 [pubmed]
PHST- 2020/07/22 06:00 [medline]
PHST- 2018/09/14 06:00 [entrez]
AID - 10.1007/s10911-018-9412-4 [pii]
AID - 10.1007/s10911-018-9412-4 [doi]
PST - ppublish
SO  - J Mammary Gland Biol Neoplasia. 2019 Mar;24(1):39-45. doi: 
      10.1007/s10911-018-9412-4. Epub 2018 Sep 12.