PMID- 30211076
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 2228-5652 (Print)
IS  - 2228-5660 (Electronic)
IS  - 2228-5652 (Linking)
VI  - 8
IP  - 3
DP  - 2018
TI  - Expression, purification and DNA-binding properties of zinc finger domains of DOF 
      proteins from Arabidopsis thaliana.
PG  - 167-176
LID - 10.15171/bi.2018.19 [doi]
AB  - Introduction: DOF proteins are a family of plant-specific transcription factors 
      with a conserved zinc finger (ZF) DNA-binding domain. Although several studies 
      have demonstrated their specific DNA binding, quantitative affinity data is not 
      available for the binding of DOF domains to their binding sites. Methods: ZF 
      domains of DOF2.1, DOF3.4, and DOF5.8 from Arabidopsis thaliana were expressed 
      and purified. Their DNA binding affinities were assessed using gel retardation 
      assays and microscale thermophoresis with two different oligonucleotide probes 
      containing one and two copies of recognition sequence AAAG. Results: DOF zinc 
      finger domains (DOF-ZFs) were shown to form independently folded structures. 
      Assessments using microscale thermophoresis demonstrated that DOF-ZFs interact 
      more tightly (~ 100 fold) with double-motif probe than the single-motif probe. 
      The overall K(d) values for the DOF3.4-ZF and DOF5.8-ZF to the double-motif probe 
      were ~2.3+/-1 and 2.5+/-1 microM, respectively. Conclusion: Studied DOF-ZF domains formed 
      stable complexes with the double-motif probe. Although DOF3.4-ZF and DOF5.8-ZF do 
      not dimerize with an appreciable affinity in the absence of DNA (judging from 
      size-exclusion and multiangle laser light scattering data), it is possible that 
      these ZFs form protein-protein contacts when bound to this oligonucleotide, 
      consistent with previous reports that DOF proteins can homo- and hetero-dimerize.
FAU - Moghaddas Sani, Hakimeh
AU  - Moghaddas Sani H
AD  - Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, 
      Tabriz, Iran.
AD  - Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, 
      Iran.
FAU - Hamzeh-Mivehroud, Maryam
AU  - Hamzeh-Mivehroud M
AD  - Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, 
      Iran.
AD  - School of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
FAU - Silva, Ana P
AU  - Silva AP
AD  - School of Life and Environmental Sciences, The University of Sydney, NSW 2006, 
      Australia.
FAU - Walshe, James L
AU  - Walshe JL
AD  - School of Life and Environmental Sciences, The University of Sydney, NSW 2006, 
      Australia.
FAU - Mohammadi, S Abolghasem
AU  - Mohammadi SA
AD  - School of Agriculture, University of Tabriz, Tabriz, Iran.
FAU - Rahbar-Shahrouziasl, Mahdyieh
AU  - Rahbar-Shahrouziasl M
AD  - Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, 
      Iran.
FAU - Abbasi, Milad
AU  - Abbasi M
AD  - Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, 
      Iran.
FAU - Jamshidi, Omid
AU  - Jamshidi O
AD  - Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, 
      Iran.
FAU - Low, Jason Kk
AU  - Low JK
AD  - School of Life and Environmental Sciences, The University of Sydney, NSW 2006, 
      Australia.
FAU - Dastmalchi, Siavoush
AU  - Dastmalchi S
AD  - Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, 
      Iran.
AD  - School of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
AD  - Faculty of Pharmacy, Near East University, POBOX:99138, Nicosia, North Cyprus, 
      Mersin 10, Turkey.
FAU - Mackay, Joel P
AU  - Mackay JP
AD  - School of Life and Environmental Sciences, The University of Sydney, NSW 2006, 
      Australia.
LA  - eng
PT  - Journal Article
DEP - 20180128
PL  - Iran
TA  - Bioimpacts
JT  - BioImpacts : BI
JID - 101558125
PMC - PMC6128974
OTO - NOTNLM
OT  - DNA binding affinity
OT  - DOF zinc finger domain
OT  - Gel retardation assay
OT  - Microscale thermophoresis
EDAT- 2018/09/14 06:00
MHDA- 2018/09/14 06:01
PMCR- 2018/01/01
CRDT- 2018/09/14 06:00
PHST- 2018/01/02 00:00 [received]
PHST- 2018/01/22 00:00 [accepted]
PHST- 2018/09/14 06:00 [entrez]
PHST- 2018/09/14 06:00 [pubmed]
PHST- 2018/09/14 06:01 [medline]
PHST- 2018/01/01 00:00 [pmc-release]
AID - 10.15171/bi.2018.19 [doi]
PST - ppublish
SO  - Bioimpacts. 2018;8(3):167-176. doi: 10.15171/bi.2018.19. Epub 2018 Jan 28.