PMID- 30211129
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220321
IS  - 2277-9175 (Print)
IS  - 2277-9175 (Electronic)
IS  - 2277-9175 (Linking)
VI  - 7
DP  - 2018
TI  - Blockade of Glutamate Receptors within the Prelimbic Cortex Attenuate 
      Concentration of Excitatory Amino Acids in the Morphine Self-administration in 
      Rats.
PG  - 116
LID - 10.4103/abr.abr_121_18 [doi]
LID - 116
AB  - BACKGROUND: The attitude of research on addiction has been done on the key role 
      of glutamate. As a regard, the prelimbic cortex (PrL) has an important role in 
      addiction, learning, and memory. We tried to investigate the level of glutamate 
      and aspartate concentration after glutamate receptors blockade in this region in 
      the morphine-addicted rats. MATERIALS AND METHODS: In this study, we examined the 
      effects of local infusion of the N-methyl-D-aspartate receptor and 
      alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid receptor antagonists, 
      2-amino-5-phosphonovaleric acid (AP5), and 6-cyano-7-nitroquinoxaline-2, 3-dione 
      (CNQX), into the PrL cortex on the level of excitatory amino acids (EAAs) and 
      glycine. After 11 days of self-administration, the prelimbic area of the brain 
      was taken out, and the EAAs and glycine concentration was measured by 
      high-performance liquid chromatography. RESULTS: Morphine resulted in the 
      significant increase in the EAAs concentration within this area (P </= 0.001). 
      Microinjection of AP5 into this region before using of morphine significantly 
      decreased the morphine-induced glutamate and aspartate concentration (P </= 0.001). 
      CNQX had the same effect and significantly reduced the EAAs concentration 
      compared to the morphine group (P </= 0.001). In addition, microinjection of AP5 
      and CNQX simultaneously increased glycine concentration (P </= 0.001). CONCLUSIONS: 
      These results show that morphine stimulates the EAAs release in the prelimbic 
      area. It seems that microinjection of AP5 or CNQX in this region is effective in 
      reducing morphine-induced EAA. It is suggested that EAA transmission in the PrL 
      cortex may be a possible target for treatment of morphine addiction.
FAU - Aboutalebi, Fateme
AU  - Aboutalebi F
AD  - Department of Animal Biology, Faculty of Biological Sciences, Kharazmi 
      University, Tehran, Iran.
FAU - Alaei, Hojjatallah
AU  - Alaei H
AD  - Department of Physiology, School of Medicine, Isfahan University of Medical 
      Sciences, Isfahan, Iran.
FAU - Oryan, Shahrbanoo
AU  - Oryan S
AD  - Department of Animal Biology, Faculty of Biological Sciences, Kharazmi 
      University, Tehran, Iran.
LA  - eng
PT  - Journal Article
DEP - 20180829
PL  - India
TA  - Adv Biomed Res
JT  - Advanced biomedical research
JID - 101586897
PMC - PMC6124215
OTO - NOTNLM
OT  - Glutamate
OT  - high-performance liquid chromatography
OT  - morphine
OT  - prelimbic area
COIS- There are no conflicts of interest.
EDAT- 2018/09/14 06:00
MHDA- 2018/09/14 06:01
PMCR- 2018/08/29
CRDT- 2018/09/14 06:00
PHST- 2018/09/14 06:00 [entrez]
PHST- 2018/09/14 06:00 [pubmed]
PHST- 2018/09/14 06:01 [medline]
PHST- 2018/08/29 00:00 [pmc-release]
AID - ABR-7-116 [pii]
AID - 10.4103/abr.abr_121_18 [doi]
PST - epublish
SO  - Adv Biomed Res. 2018 Aug 29;7:116. doi: 10.4103/abr.abr_121_18. eCollection 2018.