PMID- 30211542
OWN - NLM
STAT- MEDLINE
DCOM- 20190523
LR  - 20210109
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Linking)
VI  - 90
IP  - 20
DP  - 2018 Oct 16
TI  - Quantitative Lipoprotein Subclass and Low Molecular Weight Metabolite Analysis in 
      Human Serum and Plasma by (1)H NMR Spectroscopy in a Multilaboratory Trial.
PG  - 11962-11971
LID - 10.1021/acs.analchem.8b02412 [doi]
AB  - We report an extensive 600 MHz NMR trial of quantitative lipoprotein and 
      small-molecule measurements in human blood serum and plasma. Five centers with 
      eleven 600 MHz NMR spectrometers were used to analyze 98 samples including 20 
      quality controls (QCs), 37 commercially sourced, paired serum and plasma samples, 
      and two National Institute of Science and Technology (NIST) reference material 
      1951c replicates. Samples were analyzed using rigorous protocols for sample 
      preparation and experimental acquisition. A commercial lipoprotein subclass 
      analysis was used to quantify 105 lipoprotein subclasses and 24 low molecular 
      weight metabolites from the NMR spectra. For all spectrometers, the instrument 
      specific variance in measuring internal QCs was lower than the percentage 
      described by the National Cholesterol Education Program (NCEP) criteria for lipid 
      testing [triglycerides <2.7%; cholesterol <2.8%; low-density lipoprotein (LDL) 
      cholesterol <2.8%; high-density lipoprotein (HDL) cholesterol <2.3%], showing 
      exceptional reproducibility for direct quantitation of lipoproteins in both 
      matrixes. The average relative standard deviations (RSDs) for the 105 lipoprotein 
      parameters in the 11 instruments were 4.6% and 3.9% for the two NIST samples, 
      whereas they were 38% and 40% for the 37 commercially sourced plasmas and sera, 
      respectively, showing negligible analytical compared to biological variation. The 
      coefficient of variance (CV) obtained for the quantification of the small 
      molecules across the 11 spectrometers was below 15% for 20 out of the 24 
      metabolites analyzed. This study provides further evidence of the suitability of 
      NMR for high-throughput lipoprotein subcomponent analysis and small-molecule 
      quantitation with the exceptional required reproducibility for clinical and other 
      regulatory settings.
FAU - Jimenez, Beatriz
AU  - Jimenez B
AUID- ORCID: 0000-0003-4593-6075
AD  - The Imperial Clinical Phenotyping Centre, Division of Integrative Systems 
      Medicine and Digestive Disease, Department of Surgery and Cancer , QEQM Building, 
      Saint Mary's Hospital , London W2 1NY , United Kingdom.
FAU - Holmes, Elaine
AU  - Holmes E
AUID- ORCID: 0000-0002-0556-8389
FAU - Heude, Clement
AU  - Heude C
AD  - Phenome Centre Birmingham , University of Birmingham , Edgbaston, Birmingham B15 
      2TT , United Kingdom.
FAU - Tolson, Rose F
AU  - Tolson RF
FAU - Harvey, Nikita
AU  - Harvey N
AUID- ORCID: 0000-0002-2533-6980
FAU - Lodge, Samantha L
AU  - Lodge SL
AD  - The Imperial Clinical Phenotyping Centre, Division of Integrative Systems 
      Medicine and Digestive Disease, Department of Surgery and Cancer , QEQM Building, 
      Saint Mary's Hospital , London W2 1NY , United Kingdom.
FAU - Chetwynd, Andrew J
AU  - Chetwynd AJ
AUID- ORCID: 0000-0001-6648-6881
AD  - Phenome Centre Birmingham , University of Birmingham , Edgbaston, Birmingham B15 
      2TT , United Kingdom.
FAU - Cannet, Claire
AU  - Cannet C
AD  - Bruker Biospin GmbH , Silberstreifen, 76287 Rheinstetten , Germany.
FAU - Fang, Fang
AU  - Fang F
AD  - Bruker Biospin GmbH , Silberstreifen, 76287 Rheinstetten , Germany.
FAU - Pearce, Jake T M
AU  - Pearce JTM
AD  - The Imperial Clinical Phenotyping Centre, Division of Integrative Systems 
      Medicine and Digestive Disease, Department of Surgery and Cancer , QEQM Building, 
      Saint Mary's Hospital , London W2 1NY , United Kingdom.
FAU - Lewis, Matthew R
AU  - Lewis MR
AD  - The Imperial Clinical Phenotyping Centre, Division of Integrative Systems 
      Medicine and Digestive Disease, Department of Surgery and Cancer , QEQM Building, 
      Saint Mary's Hospital , London W2 1NY , United Kingdom.
FAU - Viant, Mark R
AU  - Viant MR
AD  - Phenome Centre Birmingham , University of Birmingham , Edgbaston, Birmingham B15 
      2TT , United Kingdom.
FAU - Lindon, John C
AU  - Lindon JC
FAU - Spraul, Manfred
AU  - Spraul M
AD  - Bruker Biospin GmbH , Silberstreifen, 76287 Rheinstetten , Germany.
FAU - Schafer, Hartmut
AU  - Schafer H
AD  - Bruker Biospin GmbH , Silberstreifen, 76287 Rheinstetten , Germany.
FAU - Nicholson, Jeremy K
AU  - Nicholson JK
AD  - The Imperial Clinical Phenotyping Centre, Division of Integrative Systems 
      Medicine and Digestive Disease, Department of Surgery and Cancer , QEQM Building, 
      Saint Mary's Hospital , London W2 1NY , United Kingdom.
LA  - eng
GR  - MC_PC_12025/MRC_/Medical Research Council/United Kingdom
GR  - MR/M009157/1/MRC_/Medical Research Council/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180927
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Lipoproteins)
RN  - 0 (Protons)
SB  - IM
MH  - Humans
MH  - Laboratories
MH  - Lipoproteins/*blood/metabolism
MH  - Molecular Weight
MH  - *Nuclear Magnetic Resonance, Biomolecular
MH  - Protons
MH  - Quality Control
EDAT- 2018/09/14 06:00
MHDA- 2019/05/24 06:00
CRDT- 2018/09/14 06:00
PHST- 2018/09/14 06:00 [pubmed]
PHST- 2019/05/24 06:00 [medline]
PHST- 2018/09/14 06:00 [entrez]
AID - 10.1021/acs.analchem.8b02412 [doi]
PST - ppublish
SO  - Anal Chem. 2018 Oct 16;90(20):11962-11971. doi: 10.1021/acs.analchem.8b02412. 
      Epub 2018 Sep 27.