PMID- 30212436 OWN - NLM STAT- MEDLINE DCOM- 20190930 LR - 20200930 IS - 1944-7884 (Electronic) IS - 1525-4135 (Print) IS - 1525-4135 (Linking) VI - 79 IP - 2 DP - 2018 Oct 1 TI - Brief Report: Safety and Tolerability of Inguinal Lymph Node Biopsy in Individuals With Acute HIV Infection in Thailand. PG - 244-248 LID - 10.1097/QAI.0000000000001780 [doi] AB - INTRODUCTION: Latent HIV reservoirs are rapidly established in lymphoid tissues during acute HIV infection (AHI). Sampling these tissues provides important information about HIV pathogenesis. This period is associated with viral replication and immune activation that may affect procedure-related adverse events (AEs). We examined the safety and tolerability of inguinal lymph node (LN) biopsy in research participants with AHI in Bangkok, Thailand. METHODS: Between 2013 and 2016, 67 AHI participants in the RV254/SEARCH010 study underwent at least one optional inguinal LN biopsy during AHI at the baseline visit and/or after antiretroviral therapy (median 48 weeks after antiretroviral therapy). Biopsy-related AEs were graded according to NIH Division of AIDS guidelines. Poisson regression was used to calculate incidence rate ratios and 95% confidence intervals to evaluate associations of demographic and HIV characteristics, procedure timing, and repetition with AE incidence. RESULTS: Of the 67 participants, 97% were male with a median age of 26. Among 78 LN biopsies (39 at baseline and 39 at follow-up), 10 (12.8%) AEs were reported: 6 (7.7%) grade 1 and 4 (5.1%) grade 2. The AEs were biopsy-site discomfort (n = 8, 10.2%) and hematoma (n = 2, 2.6%). No factors were significantly associated with AE incidence. All biopsy-related AEs were transient and self-limited. CONCLUSIONS: Inguinal LN biopsies were safe and well tolerated in mostly Thai men with AHI. As LN biopsies become an integral part of HIV research, this study provides information to participants, researchers, and institutional review boards that these samples can be safely obtained. FAU - Chintanaphol, Michelle AU - Chintanaphol M AD - Department of Neurology, Yale University, New Haven, CT. FAU - Sacdalan, Carlo AU - Sacdalan C AD - SEARCH, The Thai Red Cross AIDS Research Centre, Bangkok, Thailand. FAU - Chottanapund, Suthat AU - Chottanapund S AD - Bamrasnaradura Infectious Diseases Institute, Bangkok, Thailand. FAU - Pinyakorn, Suteeraporn AU - Pinyakorn S AD - U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD. AD - Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD. FAU - Buranapraditkun, Supranee AU - Buranapraditkun S AD - U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD. AD - Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD. AD - Division of Allergy & Clinical Immunology, Department of Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand. FAU - Crowell, Trevor A AU - Crowell TA AD - U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD. AD - Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD. FAU - Kroon, Eugene AU - Kroon E AD - SEARCH, The Thai Red Cross AIDS Research Centre, Bangkok, Thailand. FAU - Manasnayakorn, Sopark AU - Manasnayakorn S AD - Department of Surgery, Chulalongkorn University, Bangkok, Thailand. FAU - Chipman, Jeffrey G AU - Chipman JG AD - Department of Surgery, University of Minnesota, Minneapolis, MN. FAU - Schacker, Timothy W AU - Schacker TW AD - Department of Medicine, University of Minnesota, Minneapolis, MN. FAU - Michael, Nelson AU - Michael N AD - U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD. FAU - Phanuphak, Nittaya AU - Phanuphak N AD - SEARCH, The Thai Red Cross AIDS Research Centre, Bangkok, Thailand. FAU - Spudich, Serena S AU - Spudich SS AD - Department of Neurology, Yale University, New Haven, CT. FAU - Colby, Donn J AU - Colby DJ AD - SEARCH, The Thai Red Cross AIDS Research Centre, Bangkok, Thailand. FAU - Ananworanich, Jintanat AU - Ananworanich J AD - SEARCH, The Thai Red Cross AIDS Research Centre, Bangkok, Thailand. AD - U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD. AD - Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD. AD - Department of Global Health, University of Amsterdam, Amsterdam, the Netherlands. LA - eng SI - ClinicalTrials.gov/NCT00796146 GR - HHSN261200800001E/CA/NCI NIH HHS/United States GR - R01 AI125127/AI/NIAID NIH HHS/United States GR - R01 NS084911/NS/NINDS NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, Non-P.H.S. PL - United States TA - J Acquir Immune Defic Syndr JT - Journal of acquired immune deficiency syndromes (1999) JID - 100892005 SB - IM MH - Adolescent MH - Adult MH - Female MH - Groin/*pathology MH - HIV Infections/*pathology MH - Humans MH - Lymph Nodes/*virology MH - Male MH - Thailand MH - Young Adult PMC - PMC6143219 COIS- M.C. interned at Royalty Pharma. T.A.C. has received a speaker fee from Gilead Sciences. S.S.S. codirects a clinical research study that receives donated study medications from ViiV Healthcare. J.A. has received honorarium for participating in advisory meetings for ViiV Healthcare, Merck, AbbVie, and Roche. The remaining authors have no conflicts of interest to disclose. EDAT- 2018/09/14 06:00 MHDA- 2019/10/01 06:00 PMCR- 2018/09/18 CRDT- 2018/09/14 06:00 PHST- 2018/09/14 06:00 [entrez] PHST- 2018/09/14 06:00 [pubmed] PHST- 2019/10/01 06:00 [medline] PHST- 2018/09/18 00:00 [pmc-release] AID - 00126334-201810010-00015 [pii] AID - QAIV18944 [pii] AID - 10.1097/QAI.0000000000001780 [doi] PST - ppublish SO - J Acquir Immune Defic Syndr. 2018 Oct 1;79(2):244-248. doi: 10.1097/QAI.0000000000001780.