PMID- 30213462
OWN - NLM
STAT- MEDLINE
DCOM- 20190301
LR  - 20190301
IS  - 1873-3492 (Electronic)
IS  - 0009-8981 (Linking)
VI  - 487
DP  - 2018 Dec
TI  - Desirable quality-control materials for the establishment of qualified external 
      quality assessment on prenatal diagnosis of chromosomal aneuploidies.
PG  - 80-83
LID - S0009-8981(18)30493-5 [pii]
LID - 10.1016/j.cca.2018.09.016 [doi]
AB  - OBJECTIVE: To prepare desirable quality-control materials for the establishement 
      of qualified external quality assessment on fluorescence in situ hybridization 
      (FISH)-detected prenatal diagnosis of chromosomal aneuploidies. METHODS: Four 
      types of amniotic fluid cell suspensions (13-trisomy, 18-trisomy, 21-trisomy and 
      47,XXY) were mixed together by ratio to produce mosaicism with the percentages of 
      each aneuploidy as 10%, 20%, 30% and 40%, respectively. After being stored in 
      liquid nitrogen of -196  degrees C for six months, randomly selected samples were 
      incubated in 37  degrees C water, followed by cultivation, hypo-osmosis and fixation. 
      Finally, FISH detetion was applied on them before and after external laboratory 
      mailing, in step with detection on conventional case samples. RESULTS: Before 
      mailing, the positive rates of each aneuploidy described above were 12.8%, 23.6%, 
      33.8%, 44.0%, while 12.6%, 23.8%, 34.0%, 43.5% after mailing. t-test, criteria 
      for stability assessment of quality-control materials in CANS-GL03:2006, showed 
      no significant effect of external mailing on mosaicism since corresponding t 
      values are lower than threshold with significance level alpha as 0.05 and degree of 
      freedom as 10. CONCLUSION: As FISH detection showed, the mosaic cell strains 
      prepared in current study exhibited excellent stabilities after cryopreservation 
      in -196  degrees C, subculture, hypo-osmosis, fixation and external laboratory mailing, 
      demonstrating them as reliable and promising quality-control materials for the 
      establishment of a qualified external quality assessment on prenatal diagnosis of 
      chromosomal aneuploidies.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Mao, Yuchan
AU  - Mao Y
AD  - The Key Laboratory of Reproductive Genetics, Ministry of Education(Zhejiang 
      University), Department of Obstetrics and Gynecology, Women's Hospital, School of 
      Medicine, Zhejiang University, Hangzhou 310006, China.
FAU - Su, Lan
AU  - Su L
AD  - The Key Laboratory of Reproductive Genetics, Ministry of Education(Zhejiang 
      University), Department of Obstetrics and Gynecology, Women's Hospital, School of 
      Medicine, Zhejiang University, Hangzhou 310006, China.
FAU - Li, Hongge
AU  - Li H
AD  - The Key Laboratory of Reproductive Genetics, Ministry of Education(Zhejiang 
      University), Department of Obstetrics and Gynecology, Women's Hospital, School of 
      Medicine, Zhejiang University, Hangzhou 310006, China.
FAU - Yang, Yanmei
AU  - Yang Y
AD  - The Key Laboratory of Reproductive Genetics, Ministry of Education(Zhejiang 
      University), Department of Obstetrics and Gynecology, Women's Hospital, School of 
      Medicine, Zhejiang University, Hangzhou 310006, China.
FAU - Weng, Binghuan
AU  - Weng B
AD  - The Key Laboratory of Reproductive Genetics, Ministry of Education(Zhejiang 
      University), Department of Obstetrics and Gynecology, Women's Hospital, School of 
      Medicine, Zhejiang University, Hangzhou 310006, China. Electronic address: 
      5503006@zju.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20180910
PL  - Netherlands
TA  - Clin Chim Acta
JT  - Clinica chimica acta; international journal of clinical chemistry
JID - 1302422
SB  - IM
MH  - *Aneuploidy
MH  - Chromosome Disorders/*genetics
MH  - Chromosomes, Human, Pair 13/*genetics
MH  - Chromosomes, Human, Pair 18/*genetics
MH  - Chromosomes, Human, Pair 21/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Quality Control
OTO - NOTNLM
OT  - Chromosomal aneuploidies
OT  - External quality assessment
OT  - Fluorescence in situ hybridization
OT  - Prenatal diagnosis
OT  - Quality control materials
EDAT- 2018/09/15 06:00
MHDA- 2019/03/02 06:00
CRDT- 2018/09/15 06:00
PHST- 2018/04/01 00:00 [received]
PHST- 2018/08/20 00:00 [revised]
PHST- 2018/09/07 00:00 [accepted]
PHST- 2018/09/15 06:00 [pubmed]
PHST- 2019/03/02 06:00 [medline]
PHST- 2018/09/15 06:00 [entrez]
AID - S0009-8981(18)30493-5 [pii]
AID - 10.1016/j.cca.2018.09.016 [doi]
PST - ppublish
SO  - Clin Chim Acta. 2018 Dec;487:80-83. doi: 10.1016/j.cca.2018.09.016. Epub 2018 Sep 
      10.