PMID- 30213719
OWN - NLM
STAT- MEDLINE
DCOM- 20190925
LR  - 20190925
IS  - 2213-7173 (Electronic)
IS  - 2213-7165 (Linking)
VI  - 15
DP  - 2018 Dec
TI  - Genomic analysis of the first isolate of KPC-2-producing Klebsiella pneumoniae 
      from Uruguay.
PG  - 109-110
LID - S2213-7165(18)30173-5 [pii]
LID - 10.1016/j.jgar.2018.09.001 [doi]
AB  - OBJECTIVES: Since KPC-2-producing Klebsiella pneumoniae are associated with 
      successful dissemination of a major clone, defined as sequence type 258 (ST258), 
      the aim of this study was to perform whole-genome sequencing (WGS) of the first 
      colistin-resistant K. pneumoniae strain (Kpn666) carrying bla(KPC-2) identified 
      in Uruguay in 2011 in order to identify genomic and phylogenetic traits. METHODS: 
      WGS of strain Kpn666 isolated from an asymptomatic urinary tract infection was 
      performed using Illumina MiSeq, and de novo assembly was performed using SPADES 
      v.3.11. Contigs were re-ordered using the ST258 reference genome NJST258_1 
      (GenBank CP006923) and were oriented with the MAUVE Contig Mover. Twenty complete 
      genomes of K. pneumoniae identified as ST258 using the Pasteur MLST site were 
      downloaded from GenBank (May 2017). A maximum-likelihood tree was created using 
      MEGA7 based on core single nucleotide polymorphisms (SNPs) from whole-genome 
      alignment obtained with SNP sites 
      (https://github.com/sanger-pathogens/snp-sites). RESULTS: WGS analysis revealed a 
      genome of 5448179bp (5232 CDS, 108 RNAs). Phylogenetic analysis identified that 
      Kpn666 belonged to clade I lineage of ST258. Further studies also identified 
      IncR, IncFIB(K) and IncFII(K) plasmid replicons and 11 transferable associated 
      antimicrobial resistance genes (ARGs) comprising four drug classes. The mgrB gene 
      involved in colistin resistance was shown to be disrupted by insertion of an 
      IS5-like element. CONCLUSIONS: The first isolate of KPC-2-producing K. pneumoniae 
      detected in Uruguay was sequenced and the results confirm the ability of this 
      bacterium to capture several ARGs. The KPC-2 carbapenemase in Uruguay is likely 
      to have been introduced by the high-risk clone ST258.
CI  - Copyright (c) 2018 International Society for Chemotherapy of Infection and Cancer. 
      Published by Elsevier Ltd. All rights reserved.
FAU - Alvarez, Veronica E
AU  - Alvarez VE
AD  - Instituto de Microbiologia y Parasitologia Medica (IMPaM, UBA-CONICET), Facultad 
      de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina.
FAU - Campos, Josefina
AU  - Campos J
AD  - Plataforma de genomica y bioinformatica, INEI-ANLIS 'Dr Carlos G. Malbran', 
      Buenos Aires, Argentina.
FAU - Galiana, Antonio
AU  - Galiana A
AD  - Departamento de Microbiologia, Hospital Maciel, UDYCI, Montevideo, Uruguay.
FAU - Borthagaray, Graciela
AU  - Borthagaray G
AD  - Catedra de Microbiologia, Facultad de Quimica-UdelaR, Montevideo, Uruguay.
FAU - Centron, Daniela
AU  - Centron D
AD  - Instituto de Microbiologia y Parasitologia Medica (IMPaM, UBA-CONICET), Facultad 
      de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina.
FAU - Marquez Villalba, Carolina
AU  - Marquez Villalba C
AD  - Catedra de Microbiologia, Instituto de Quimica Biologica, Facultad de Ciencias y 
      de Quimica-UdelaR, Montevideo, Uruguay. Electronic address: cmarquez@fq.edu.uy.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180910
PL  - Netherlands
TA  - J Glob Antimicrob Resist
JT  - Journal of global antimicrobial resistance
JID - 101622459
RN  - EC 3.5.2.- (beta-lactamase KPC-2)
RN  - EC 3.5.2.6 (beta-Lactamases)
RN  - Z67X93HJG1 (Colistin)
SB  - IM
MH  - Colistin/pharmacology
MH  - *Genome, Bacterial
MH  - Genomics
MH  - Humans
MH  - Klebsiella Infections/microbiology
MH  - Klebsiella pneumoniae/drug effects/*enzymology/*genetics
MH  - Multilocus Sequence Typing
MH  - *Phylogeny
MH  - Urinary Tract Infections/microbiology
MH  - Uruguay
MH  - beta-Lactamases/*biosynthesis/genetics/isolation & purification
OTO - NOTNLM
OT  - KPC
OT  - Klebsiella pneumoniae
OT  - ST258
EDAT- 2018/09/15 06:00
MHDA- 2019/09/26 06:00
CRDT- 2018/09/15 06:00
PHST- 2018/05/25 00:00 [received]
PHST- 2018/08/15 00:00 [revised]
PHST- 2018/09/04 00:00 [accepted]
PHST- 2018/09/15 06:00 [pubmed]
PHST- 2019/09/26 06:00 [medline]
PHST- 2018/09/15 06:00 [entrez]
AID - S2213-7165(18)30173-5 [pii]
AID - 10.1016/j.jgar.2018.09.001 [doi]
PST - ppublish
SO  - J Glob Antimicrob Resist. 2018 Dec;15:109-110. doi: 10.1016/j.jgar.2018.09.001. 
      Epub 2018 Sep 10.