PMID- 30214523
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 16
IP  - 4
DP  - 2018 Oct
TI  - Effect of autophagy-associated proteins on the arecoline-induced liver injury in 
      mice.
PG  - 3041-3049
LID - 10.3892/etm.2018.6564 [doi]
AB  - Arecoline can be used to treat diseases including glaucoma and tapeworm 
      infection, however, long-term administration can cause severe adverse effects, 
      including oral submucosal fibrosis, oral cancer, hepatic injury and liver cancer. 
      Autophagy serves a role in these injuries. The present study established a mouse 
      model of arecoline-induced hepatic injury and investigated the role of 
      autophagy-associated proteins in this injury. The results indicated that the 
      expression levels of the autophagy marker protein microtubule associated protein 
      1 light chain 3 B (MAP1LC3B) and autophagy-promoting protein beclin 1 were 
      elevated in the injured hepatic cells, while the expression levels of a 
      well-known negative regulator of autophagy, mammalian target of rapamycin (mTOR), 
      were reduced. Following treatment of the hepatic injury with glutathione, the 
      liver function improved and liver damage was reduced effectively. Compared with 
      the control group, the expression levels of both MAP1LC3B and beclin 1 were 
      significantly upregulated in the glutathione-treated mice, but the expression of 
      mTOR was significantly downregulated. It may be concluded that in the process of 
      protecting against arecoline-induced hepatic injury, glutathione cooperates with 
      mTOR and beclin 1 to accelerate autophagy, maintaining stable cell morphology and 
      cellular functions.
FAU - Wang, Xia
AU  - Wang X
AD  - Department of Pathology, Binzhou Medical University, Yantai, Shandong 264003, 
      P.R. China.
FAU - Song, Xinhong
AU  - Song X
AD  - Department of Radiology, Yantai Affiliated Hospital of Binzhou Medical 
      University, Yantai, Shandong 264100, P.R. China.
FAU - Si, Youjiao
AU  - Si Y
AD  - Department of Radiology, Yantai Affiliated Hospital of Binzhou Medical 
      University, Yantai, Shandong 264100, P.R. China.
FAU - Xia, Jikai
AU  - Xia J
AD  - Department of Radiology, Yantai Affiliated Hospital of Binzhou Medical 
      University, Yantai, Shandong 264100, P.R. China.
FAU - Wang, Bin
AU  - Wang B
AD  - Medical Imaging Research Institute, Binzhou Medical University, Yantai, Shandong 
      264003, P.R. China.
FAU - Wang, Peiyuan
AU  - Wang P
AD  - Department of Radiology, Yantai Affiliated Hospital of Binzhou Medical 
      University, Yantai, Shandong 264100, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20180802
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC6125830
OTO - NOTNLM
OT  - arecoline
OT  - autophagy
OT  - glutathione
OT  - hepatic injury
EDAT- 2018/09/15 06:00
MHDA- 2018/09/15 06:01
PMCR- 2018/08/02
CRDT- 2018/09/15 06:00
PHST- 2017/11/05 00:00 [received]
PHST- 2018/06/29 00:00 [accepted]
PHST- 2018/09/15 06:00 [entrez]
PHST- 2018/09/15 06:00 [pubmed]
PHST- 2018/09/15 06:01 [medline]
PHST- 2018/08/02 00:00 [pmc-release]
AID - ETM-0-0-6564 [pii]
AID - 10.3892/etm.2018.6564 [doi]
PST - ppublish
SO  - Exp Ther Med. 2018 Oct;16(4):3041-3049. doi: 10.3892/etm.2018.6564. Epub 2018 Aug 
      2.