PMID- 30216582
OWN - NLM
STAT- MEDLINE
DCOM- 20190923
LR  - 20211204
IS  - 1098-2795 (Electronic)
IS  - 1040-452X (Linking)
VI  - 85
IP  - 10
DP  - 2018 Oct
TI  - Upregulation of HB-EGF, Msx.1, and miRNA Let-7a by administration of calcitonin 
      through mTOR and ERK1/2 pathways during a window of implantation in mice.
PG  - 790-801
LID - 10.1002/mrd.23061 [doi]
AB  - BACKGROUND: Successful implantation of embryos requires endometrial receptivity. 
      Calcitonin is one of the factors influencing the implantation window. This study 
      aimed to evaluate calcitonin effects on endometrial receptivity. To this end, the 
      effects of calcitonin on the implantation window in the ovarian stimulation and 
      the normal ovarian cycle were investigated by the morphological study of the 
      endometrium as well as the expression of MSX.1, HB-EGF, and micro-RNA (miRNA) 
      Let-7a; then the mechanisms of calcitonin effects were studied through the 
      mammalian target of rapamycin (mTOR) and extracellular signal-regulated kinase 
      1/2 (ERK1/2) signaling pathways. MATERIALS AND METHODS: A total of 64 Bulb/c mice 
      were divided into two groups: Normal ovarian cycle and ovarian stimulation. Each 
      group consisted of four subgroups: Ctrl, CT, PP242, and CT + PP242. Calcitonin 
      and PP242 were injected on the fourth day of pregnancy and 24 hr later all the 
      mice were killed. Uterine tissue samples were used for morphological analysis and 
      the endometrial epithelial and the stromal cells were isolated from myometrium 
      for evaluation of gene and protein expression. RESULTS: Ovarian stimulation 
      increased the phosphorylation levels of mTOR and ERK1/2 and the expression of 
      miRNA Let-7a. Calcitonin injection increased the expression of HB-EGF, Msx.1, and 
      miRNA Let-7a in a normal ovarian cycle and in ovarian-stimulated mice. It also 
      increased eukaryotic initiation factor 4E-binding protein 1 and ERK1/2 
      phosphorylation in normal ovarian cycles. CONCLUSION: Calcitonin improved the 
      receptivity of the uterine endometrium by upregulation of the HB-EGF, Msx.1, and 
      miRNA Let-7a likely through mTOR and ERK1/2 signaling pathway.
CI  - (c) 2018 Wiley Periodicals, Inc.
FAU - Shokrzadeh, Naser
AU  - Shokrzadeh N
AUID- ORCID: 0000-0002-8816-2797
AD  - Immunology Research Center, Faculty Of Medicine, Tabriz University of Medical 
      Sciences, Tabriz, Iran.
FAU - Alivand, Mohammad Reza
AU  - Alivand MR
AD  - Department of Medical Genetics, Faculty of Medicine, Tabriz University of Medical 
      Sciences, Tabriz, Iran.
FAU - Abedelahi, Ali
AU  - Abedelahi A
AD  - Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of 
      Medical Sciences, Tabriz, Iran.
FAU - Hessam Shariati, Mohammad Bakhtiar
AU  - Hessam Shariati MB
AD  - Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of 
      Medical Sciences, Tabriz, Iran.
FAU - Niknafs, Behrooz
AU  - Niknafs B
AD  - Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of 
      Medical Sciences, Tabriz, Iran.
LA  - eng
GR  - 58330/Immunology Research Center, Faculty of Medicine, Tabriz University of 
      Medical Sciences, Tabriz, Iran/International
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Reprod Dev
JT  - Molecular reproduction and development
JID - 8903333
RN  - 0 (Heparin-binding EGF-like Growth Factor)
RN  - 0 (MSX1 Transcription Factor)
RN  - 0 (MicroRNAs)
RN  - 0 (Msx1 protein, mouse)
RN  - 0 (mirnlet7 microRNA, mouse)
RN  - 9007-12-9 (Calcitonin)
RN  - EC 2.7.1.1 (mTOR protein, mouse)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - EC 2.7.11.24 (Mapk1 protein, mouse)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3)
SB  - IM
MH  - Animals
MH  - Calcitonin/*pharmacology
MH  - Embryo Implantation/*drug effects
MH  - Endometrium/*metabolism
MH  - Female
MH  - Heparin-binding EGF-like Growth Factor/*biosynthesis
MH  - MAP Kinase Signaling System/*drug effects
MH  - MSX1 Transcription Factor/*biosynthesis
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - MicroRNAs/*biosynthesis
MH  - Mitogen-Activated Protein Kinase 1/metabolism
MH  - Mitogen-Activated Protein Kinase 3/metabolism
MH  - Pregnancy
MH  - TOR Serine-Threonine Kinases/*biosynthesis
MH  - Up-Regulation/*drug effects
OTO - NOTNLM
OT  - Calcitonin
OT  - ERK1/2
OT  - HB-EGF
OT  - Msx.1
OT  - mTOR
OT  - miRNA Let-7a
OT  - window of implantation
EDAT- 2018/09/15 06:00
MHDA- 2019/09/24 06:00
CRDT- 2018/09/15 06:00
PHST- 2018/08/07 00:00 [received]
PHST- 2018/09/12 00:00 [accepted]
PHST- 2018/09/15 06:00 [pubmed]
PHST- 2019/09/24 06:00 [medline]
PHST- 2018/09/15 06:00 [entrez]
AID - 10.1002/mrd.23061 [doi]
PST - ppublish
SO  - Mol Reprod Dev. 2018 Oct;85(10):790-801. doi: 10.1002/mrd.23061.