PMID- 30217408
OWN - NLM
STAT- MEDLINE
DCOM- 20190208
LR  - 20190215
IS  - 1618-0372 (Electronic)
IS  - 0065-1281 (Linking)
VI  - 120
IP  - 8
DP  - 2018 Nov
TI  - Expression profile of some neuronal and glial cell markers in the ovine ileal 
      enteric nervous system during prenatal development.
PG  - 768-779
LID - S0065-1281(18)30173-9 [pii]
LID - 10.1016/j.acthis.2018.09.002 [doi]
AB  - The enteric nervous system (ENS) is a network of neurons and glia found in the 
      gut wall and governs this gastrointestinal function independently from the 
      central nervous system (CNS). ENS comprises the myenteric plexus (MP) and the 
      submucous plexus (SP). In this study, we examined the expression profile of 
      neurofilament heavy chain (NF-H), neuron-specific enolase (NSE), calcyclin 
      (S100A6), vimentin and glial fibril acidic protein (GFAP) in ovine ileal enteric 
      neurons and enteric glia cells (EGCs) during prenatal development using an 
      immunohistochemical method. The material of the study consisted of 15 different 
      fetal ileum tissues obtained between days 60 and 150 of pregnancy. NF-H was 
      observed in the majority of ganglion cells in SP and MP throughout the fetal 
      period. It was determined that there was no NF-H reaction in some ganglion cells 
      in Peyer's patches of internal submucosal plexus (ISPF). In the early stage of 
      pregnancy (60-90 days), there was no expression of NSE and S1006 in ileum. After 
      this period, NSE and S1006 were expressed in the ganglion cells of the plexus, 
      indicating an increase in the amount of expression towards the end of pregnancy. 
      In the early period, vimentin expression was only detected in intramuscular 
      interstitial cells (ICs) (60-90 days), but later (90-150 days) it was also seen 
      in the cells around the ganglion cells in the plexus. On days 60-90 of gestation, 
      GFAP expression only occurred in MP, but in later stages, staining was also 
      detected in SP. In the plexus, an immunoreactivity was present in EGCs forming a 
      network around the ganglion cell. During the last period of gestation (120-150 
      days), the number of GFAP-positive plexus increased, with the majority of these 
      stained cells being observed in MP. Interestingly, weak staining or reaction did 
      not occur in ISPF, unlike other plexuses. In conclusion, this is the first study 
      that demonstrated the expression of NF-H, vimentin, S100A6, NSE and glial fibril 
      acidic protein (GFAP) in ovine ileal ENS in the prenatal period. In the last 
      period of gestation (120-150 days), the expression profile of ENS was similar to 
      that of adult animals. The expression of the used markers increased toward the 
      end of pregnancy. Our results suggest that neurons and EGCs show heterogeneity, 
      and GFAP and NF-H cannot be used as panenteric glial or panneuronal markers, 
      respectively. We also demonstrated, for the first time, the prenatal expression 
      of S100A6 in enteric neurons and the possibility of using this protein for the 
      identification of enteric neurons.
CI  - Copyright (c) 2018. Published by Elsevier GmbH.
FAU - Ozbek, Mehmet
AU  - Ozbek M
AD  - Deparment of Histology and Embryology, Faculty of Veterinary Medicine, Mehmet 
      Akif Ersoy University, Istiklal Yerleskesi, 15030, Burdur, Turkey. Electronic 
      address: mozbek@mehmetakif.edu.tr.
FAU - Bozkurt, Mehmet Fatih
AU  - Bozkurt MF
AD  - Department of Pathology, Faculty of Veterinary Medicine, Afyon Kocatepe 
      University, Afyonkarahisar, Turkey.
FAU - Beyaz, Feyzullah
AU  - Beyaz F
AD  - Deparment of Histology and Embryology, Faculty of Veterinary Medicine, Erciyes 
      University, Kayseri, Turkey.
FAU - Ergun, Emel
AU  - Ergun E
AD  - Deparment of Histology and Embryology, Faculty of Veterinary Medicine, Ankara 
      University, Ankara, Turkey.
FAU - Ergun, Levent
AU  - Ergun L
AD  - Deparment of Histology and Embryology, Faculty of Veterinary Medicine, Ankara 
      University, Ankara, Turkey.
LA  - eng
PT  - Journal Article
DEP - 20180911
PL  - Germany
TA  - Acta Histochem
JT  - Acta histochemica
JID - 0370320
RN  - 0 (Biomarkers)
RN  - 0 (GFAP protein, human)
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (Vimentin)
SB  - IM
MH  - Animals
MH  - Biomarkers/metabolism
MH  - Enteric Nervous System/growth & development/*metabolism
MH  - Female
MH  - Glial Fibrillary Acidic Protein/metabolism
MH  - Ileum/growth & development/metabolism
MH  - Immunohistochemistry
MH  - Neuroglia/*metabolism
MH  - Neurons/*metabolism
MH  - Pregnancy
MH  - Sheep
MH  - Vimentin/metabolism
OTO - NOTNLM
OT  - Enteric nervous system
OT  - S100A6
OT  - Sheep
EDAT- 2018/09/16 06:00
MHDA- 2019/02/09 06:00
CRDT- 2018/09/16 06:00
PHST- 2018/05/17 00:00 [received]
PHST- 2018/08/07 00:00 [revised]
PHST- 2018/09/03 00:00 [accepted]
PHST- 2018/09/16 06:00 [pubmed]
PHST- 2019/02/09 06:00 [medline]
PHST- 2018/09/16 06:00 [entrez]
AID - S0065-1281(18)30173-9 [pii]
AID - 10.1016/j.acthis.2018.09.002 [doi]
PST - ppublish
SO  - Acta Histochem. 2018 Nov;120(8):768-779. doi: 10.1016/j.acthis.2018.09.002. Epub 
      2018 Sep 11.