PMID- 30221237
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 2470-1343 (Print)
IS  - 2470-1343 (Electronic)
IS  - 2470-1343 (Linking)
VI  - 3
IP  - 7
DP  - 2018 Jul 31
TI  - Controlled Dye Aggregation in Sodium Dodecylsulfate-Stabilized 
      Poly(methylmethacrylate) Nanoparticles as Fluorescent Imaging Probes.
PG  - 7663-7672
LID - 10.1021/acsomega.8b00785 [doi]
AB  - Polymer nanoparticles are used extensively in biomedical applications. 
      Poly(methylmethacrylate) (PMMA) nanoparticles obtained via nanoprecipitation were 
      unstable and flocculate or precipitate from solution within a few hours. A simple 
      method to improve the stability of the particles using surfactants at low 
      concentrations was carried out to produce PMMA nanoparticles with long-term 
      stability in water (>6 months). The increased stability was attributed to the 
      incorporation of surfactants inside the polymer particles during 
      nanoprecipitation. The same methodology was also adopted to encapsulate a highly 
      fluorescent hydrophobic perylene tetraester inside the polymer nanoparticles with 
      good stability in water. Because of the presence of the anionic sodium dodecyl 
      sulfate, the particles showed a negative zeta potential of -34.7 mV and an 
      average size of 150 nm. Similarly, the dye-encapsulated polymer nanoparticles 
      showed a zeta potential of -35.1 mV and an average particle size of 180 nm. By 
      varying the concentration of encapsulated dyes inside the polymer nanoparticles, 
      dye aggregation could be controlled, and the fluorescence profiles of the 
      nanoparticles were altered. To understand the uptake and toxicity of the polymer 
      nanoparticles, baby hamster kidney cells were chosen as a model system. The 
      polymer nanoparticles were taken up by the cells within 3 h and were nontoxic at 
      concentrations as high as 100 ppm. The confocal micrographs of the cells revealed 
      localized fluorescence from the polymer nanoparticles around the nucleus in the 
      cytoplasm without the penetration of the nuclear envelope.
FAU - Bhargava, Samarth
AU  - Bhargava S
AD  - Department of Chemistry, National University of Singapore, 3 Science Drive 3, 
      117543, Singapore.
FAU - Chu, Justin Jang Hann
AU  - Chu JJH
AD  - Department of Microbiology and Immunology, National University of Singapore, 5 
      Science Drive 2, 117545, Singapore.
FAU - Valiyaveettil, Suresh
AU  - Valiyaveettil S
AD  - Department of Chemistry, National University of Singapore, 3 Science Drive 3, 
      117543, Singapore.
LA  - eng
PT  - Journal Article
DEP - 20180711
PL  - United States
TA  - ACS Omega
JT  - ACS omega
JID - 101691658
PMC - PMC6130898
COIS- The authors declare no competing financial interest.
EDAT- 2018/09/18 06:00
MHDA- 2018/09/18 06:01
PMCR- 2018/07/11
CRDT- 2018/09/18 06:00
PHST- 2018/04/22 00:00 [received]
PHST- 2018/06/26 00:00 [accepted]
PHST- 2018/09/18 06:00 [entrez]
PHST- 2018/09/18 06:00 [pubmed]
PHST- 2018/09/18 06:01 [medline]
PHST- 2018/07/11 00:00 [pmc-release]
AID - 10.1021/acsomega.8b00785 [doi]
PST - ppublish
SO  - ACS Omega. 2018 Jul 31;3(7):7663-7672. doi: 10.1021/acsomega.8b00785. Epub 2018 
      Jul 11.