PMID- 30223109
OWN - NLM
STAT- MEDLINE
DCOM- 20190111
LR  - 20190111
IS  - 1872-7077 (Electronic)
IS  - 1382-6689 (Linking)
VI  - 63
DP  - 2018 Oct
TI  - Cellular uptake and apoptotic potential of rhenium labeled magnetic protein cages 
      in MDA-MB-231 cells.
PG  - 127-134
LID - S1382-6689(18)30284-9 [pii]
LID - 10.1016/j.etap.2018.08.014 [doi]
AB  - (188)Re-magnetoferritin nanoparticles (NPs) provide an attractive platform for 
      localized radiation therapy due to their magnetic targeting capability while 
      enhancing contrast in magnetic resonans imaging (MRI) signals. In this study, 
      cellular uptake, in vitro cytotoxicity, apoptotic potential of a non-radioactive 
      isotope of rhenium in the form of (187)Re-magnetoferritin NPs were evaluated in 
      both human normal mammary epithelial and breast metastatic adenocarcinoma cell 
      lines. The results showed that, NP administration into the cells is through 
      receptor mediated endocytosis and cancer cells displayed significantly higher 
      uptake and cytotoxicity compared to normal cells. IC(50) values of nanoparticles 
      were calculated as 0.96 mg/mL for cancer and 1.73 mg/mL for normal cells. Annexin 
      V/ Propidium Iodide (PI) staining also showed that, NPs induced higher apoptotic 
      rates in cancer cells compared to normal cells. Gene expression analyses 
      confirming the results showed that, pro-apoptotic PUMA and BAX genes were 
      significantly up-regulated while anti-apoptotic BCL-2 and SURVIVIN genes were 
      down-regulated in cancer cells compared to normal cells. Overall, these in vitro 
      results suggest that, (187)Re-magnetoferritin NPs have a promising potential for 
      cancer therapy and can be used for imaging and diagnostic purposes for breast 
      cancer at concentrations lower than 0.96 mg/mL. At concentrations above 1 mg/mL, 
      NPs induce apoptosis which can also be used for cancer treatments.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Asik, Elif
AU  - Asik E
AD  - Department of Biotechnology, Middle East Technical University, Ankara, 06800, 
      Turkey.
FAU - Aslan, Tugba Nur
AU  - Aslan TN
AD  - Department of Chemistry, Middle East Technical University, Ankara, 06800, Turkey.
FAU - Guray, N Tulin
AU  - Guray NT
AD  - Department of Biotechnology, Middle East Technical University, Ankara, 06800, 
      Turkey; Department of Biological Sciences, Middle East Technical University, 
      Ankara, 06800, Turkey.
FAU - Volkan, Murvet
AU  - Volkan M
AD  - Department of Chemistry, Middle East Technical University, Ankara, 06800, Turkey. 
      Electronic address: murvet@metu.edu.tr.
LA  - eng
PT  - Journal Article
DEP - 20180825
PL  - Netherlands
TA  - Environ Toxicol Pharmacol
JT  - Environmental toxicology and pharmacology
JID - 9612020
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - 0 (BAX protein, human)
RN  - 0 (BBC3 protein, human)
RN  - 0 (BCL2 protein, human)
RN  - 0 (BIRC5 protein, human)
RN  - 0 (Magnetite Nanoparticles)
RN  - 0 (Oxides)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (Radioisotopes)
RN  - 0 (Survivin)
RN  - 0 (bcl-2-Associated X Protein)
RN  - 0 (magnetoferritin)
RN  - 229C124H5I (Rhenium-188)
RN  - 7440-15-5 (Rhenium)
RN  - 9013-31-4 (Apoferritins)
RN  - E1UOL152H7 (Iron)
SB  - IM
MH  - Apoferritins/*chemistry
MH  - Apoptosis Regulatory Proteins/*genetics
MH  - Breast Neoplasms/drug therapy/*genetics
MH  - Cell Line, Tumor
MH  - Cell Survival/drug effects
MH  - Drug Screening Assays, Antitumor
MH  - Endocytosis
MH  - Female
MH  - Gene Expression Regulation, Neoplastic/drug effects
MH  - Humans
MH  - Iron/*chemistry
MH  - Magnetite Nanoparticles/chemistry
MH  - Oxides/*chemistry
MH  - Proto-Oncogene Proteins/genetics
MH  - Proto-Oncogene Proteins c-bcl-2/genetics
MH  - Radioisotopes/chemistry/*pharmacology
MH  - Rhenium/chemistry/*pharmacology
MH  - Survivin/genetics
MH  - bcl-2-Associated X Protein/genetics
OTO - NOTNLM
OT  - Apoptosis
OT  - Breast cancer cell lines
OT  - Cytotoxicity
OT  - Magnetoferritin
EDAT- 2018/09/18 06:00
MHDA- 2019/01/12 06:00
CRDT- 2018/09/18 06:00
PHST- 2018/06/06 00:00 [received]
PHST- 2018/08/20 00:00 [revised]
PHST- 2018/08/23 00:00 [accepted]
PHST- 2018/09/18 06:00 [pubmed]
PHST- 2019/01/12 06:00 [medline]
PHST- 2018/09/18 06:00 [entrez]
AID - S1382-6689(18)30284-9 [pii]
AID - 10.1016/j.etap.2018.08.014 [doi]
PST - ppublish
SO  - Environ Toxicol Pharmacol. 2018 Oct;63:127-134. doi: 10.1016/j.etap.2018.08.014. 
      Epub 2018 Aug 25.