PMID- 30225677
OWN - NLM
STAT- MEDLINE
DCOM- 20191220
LR  - 20200225
IS  - 1436-3771 (Electronic)
IS  - 1432-6981 (Linking)
VI  - 23
IP  - 4
DP  - 2019 Apr
TI  - Immunostimulatory activity of low-molecular-weight hyaluronan on dendritic cells 
      stimulated with Aggregatibacter actinomycetemcomitans or Porphyromonas 
      gingivalis.
PG  - 1887-1894
LID - 10.1007/s00784-018-2641-5 [doi]
AB  - OBJECTIVES: Periodontitis is a chronic inflammatory disease characterized by 
      tooth-supporting tissue destruction, which is elicited by the host's immune 
      response triggered against periodonto-pathogen bacteria. During periodontal 
      tissue destruction, extracellular matrix components are metabolized and 
      fragmented. Some extracellular matrix component-derived fragments, such as 
      low-molecular-weight hyaluronan (LMW-HA), have potent immunogenic potential, 
      playing a role as damage-associated molecular patterns (DAMPs) during activation 
      of immune cells. Dendritic cells (DCs) play a central role in the host's immune 
      response displayed during periodontitis; thus, this study aimed to analyze 
      whether LMW-HA has an immunostimulatory activity on DCs when stimulated with 
      periodonto-pathogen bacteria. MATERIALS AND METHODS: LMW-HA-treated and 
      non-treated DCs were stimulated with Aggregatibacter actinomycetemcomitans or 
      Porphyromonas gingivalis and the mRNA expression for cytokines tumor necrosis 
      factor-alpha (TNF-alpha), interleukin-1beta (IL-1B), interleukin-6 (IL-6), and 
      interleukin-23 (IL-23A) was quantified by RT-qPCR. In addition, transcription 
      factors interferon regulatory factor 4 (IRF4), interferon regulatory factor 8 
      (IRF8), neurogenic locus notch homolog protein 2 (NOTCH2), and basic leucine 
      zipper ATF-like transcription factor 3 (BATF3), involved in DC activation, were 
      analyzed. RESULTS: Higher expression levels of TNF-alpha, IL-1B, IL-6, and IL-23A 
      were detected in LMW-HA-treated DCs after bacterial infection, as compared with 
      non-treated DCs. When LMW-HA-treated DCs were infected with A. 
      actinomycetemcomitans, higher levels of IRF4, NOTCH2, and BATF3 were detected 
      compared with non-treated cells; whereas against P. gingivalis infection, 
      increased levels of IRF4 and NOTCH2 were detected. CONCLUSION: LMW-HA plays an 
      immunostimulatory role on the immune response triggered by DCs during infection 
      with A. actinomycetemcomitans or P. gingivalis. CLINICAL RELEVANCE: Detection of 
      extracellular matrix component-derived fragments produced during periodontal 
      tissue destruction, such as LMW-HA, could explain at least partly unsuccessful 
      periodontal treatment and the chronicity of the disease.
FAU - Monasterio, Gustavo
AU  - Monasterio G
AD  - Periodontal Biology Laboratory, Faculty of Dentistry, Universidad de Chile, 
      Sergio Livingstone Pohlhammer 943, 8380492, Independencia, Santiago, Chile.
FAU - Guevara, Jose
AU  - Guevara J
AD  - Periodontal Biology Laboratory, Faculty of Dentistry, Universidad de Chile, 
      Sergio Livingstone Pohlhammer 943, 8380492, Independencia, Santiago, Chile.
FAU - Ibarra, Juan Pablo
AU  - Ibarra JP
AD  - Periodontal Biology Laboratory, Faculty of Dentistry, Universidad de Chile, 
      Sergio Livingstone Pohlhammer 943, 8380492, Independencia, Santiago, Chile.
FAU - Castillo, Francisca
AU  - Castillo F
AD  - Periodontal Biology Laboratory, Faculty of Dentistry, Universidad de Chile, 
      Sergio Livingstone Pohlhammer 943, 8380492, Independencia, Santiago, Chile.
FAU - Diaz-Zuniga, Jaime
AU  - Diaz-Zuniga J
AD  - Periodontal Biology Laboratory, Faculty of Dentistry, Universidad de Chile, 
      Sergio Livingstone Pohlhammer 943, 8380492, Independencia, Santiago, Chile.
FAU - Alvarez, Carla
AU  - Alvarez C
AD  - Periodontal Biology Laboratory, Faculty of Dentistry, Universidad de Chile, 
      Sergio Livingstone Pohlhammer 943, 8380492, Independencia, Santiago, Chile.
FAU - Cafferata, Emilio A
AU  - Cafferata EA
AD  - Periodontal Biology Laboratory, Faculty of Dentistry, Universidad de Chile, 
      Sergio Livingstone Pohlhammer 943, 8380492, Independencia, Santiago, Chile.
AD  - Faculty of Dentistry, Universidad Peruana Cayetano Heredia, Lima, Peru.
FAU - Vernal, Rolando
AU  - Vernal R
AUID- ORCID: 0000-0002-1391-320X
AD  - Periodontal Biology Laboratory, Faculty of Dentistry, Universidad de Chile, 
      Sergio Livingstone Pohlhammer 943, 8380492, Independencia, Santiago, Chile. 
      rvernal@uchile.cl.
AD  - Dentistry Unit, Faculty of Health Sciences, Universidad Autonoma de Chile, 
      Santiago, Chile. rvernal@uchile.cl.
LA  - eng
GR  - FONDECYT 1181780/Comision Nacional de Investigacion Cientifica y Tecnologica 
      (CONICYT)/
GR  - FONDECYT 1140904/Comision Nacional de Investigacion Cientifica y Tecnologica 
      (CONICYT)/
PT  - Journal Article
DEP - 20180917
PL  - Germany
TA  - Clin Oral Investig
JT  - Clinical oral investigations
JID - 9707115
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (Cytokines)
RN  - 9004-61-9 (Hyaluronic Acid)
MH  - Adjuvants, Immunologic/*pharmacology
MH  - *Aggregatibacter actinomycetemcomitans
MH  - Cells, Cultured
MH  - Cytokines/immunology
MH  - Dendritic Cells/*drug effects/microbiology
MH  - Extracellular Matrix
MH  - Humans
MH  - Hyaluronic Acid/*pharmacology
MH  - Molecular Weight
MH  - Periodontitis
MH  - *Porphyromonas gingivalis
OTO - NOTNLM
OT  - Aggregatibacter actinomycetemcomitans
OT  - Cytokines
OT  - Dendritic cells
OT  - Hyaluronan
OT  - LMW-HA
OT  - Porphyromonas gingivalis
OT  - Transcription factors
EDAT- 2018/09/19 06:00
MHDA- 2019/12/21 06:00
CRDT- 2018/09/19 06:00
PHST- 2018/05/07 00:00 [received]
PHST- 2018/09/11 00:00 [accepted]
PHST- 2018/09/19 06:00 [pubmed]
PHST- 2019/12/21 06:00 [medline]
PHST- 2018/09/19 06:00 [entrez]
AID - 10.1007/s00784-018-2641-5 [pii]
AID - 10.1007/s00784-018-2641-5 [doi]
PST - ppublish
SO  - Clin Oral Investig. 2019 Apr;23(4):1887-1894. doi: 10.1007/s00784-018-2641-5. 
      Epub 2018 Sep 17.