PMID- 30226056
OWN - NLM
STAT- MEDLINE
DCOM- 20181019
LR  - 20181019
IS  - 1520-5118 (Electronic)
IS  - 0021-8561 (Linking)
VI  - 66
IP  - 40
DP  - 2018 Oct 10
TI  - Walnut ( Juglans regia) Peptides Reverse Sleep Deprivation-Induced Memory 
      Impairment in Rat via Alleviating Oxidative Stress.
PG  - 10617-10627
LID - 10.1021/acs.jafc.8b03884 [doi]
AB  - The aim of this study was to determine the neuroprotective effects of walnut 
      protein hydrolysates (WPH) against memory deficits induced by sleep deprivation 
      (SD) in rat and further to identify and characterize the potent neuroprotective 
      peptides against glutamate-induced apoptosis in PC12 cells. Results showed that a 
      remarkable amelioration effect on behavioral performance in Morris water maze 
      test was observed for WPH and its low molecular weight fraction WPHL, especially 
      for WPHL. Additionally, a reduction of antioxidant defense (catalase, glutathione 
      peroxidase (GSH-px), and superoxide dismutase (SOD)) and an increase of 
      malondialdehyde content induced by SD were normalized in brain of rat after oral 
      administration of WPH and WPHL. Then three neuroprotective peptides including 
      GGW, VYY, and LLPF were identified from WPHL, which could protect PC12 cells 
      against glutamate-induced apoptosis with relative cell viability of 78.29 +/- 
      3.09%, 80.65 +/- 1.74%, and 83.97 +/- 3.06%, respectively, versus glutamate group 
      48.61 +/- 3.99%. The possible mechanism underlying their protective effects of GGW 
      and VYY could be related to their strong radical scavenging activity as well as 
      their ability to reduce reactive oxygen species production and the depletion of 
      SOD and GSH-px in PC12 cells. Notably, the marked neuroprotective effects of 
      LLPF, which did not show obvious free-radical scavenging activity in vitro, could 
      be attributed to its strong effects on inhibiting Ca(2+) influx and mitochondrial 
      membrane potential collapse. Additionally, all these peptides could regulate the 
      expression of apoptosis-related proteins (Bax and Bcl-2). Therefore, walnut 
      peptides might be regarded as the potential nutraceuticals against 
      neurodegenerative disorders associated with memory deficits.
FAU - Wang, Shuguang
AU  - Wang S
AD  - School of Food Science and Engineering , South China University of Technology , 
      Guangzhou 510640 , China.
AD  - Guangdong Food Green Processing and Nutrition Regulation Technologies Research 
      Center , Guangzhou 510650 , China.
FAU - Su, Guowan
AU  - Su G
AD  - School of Food Science and Engineering , South China University of Technology , 
      Guangzhou 510640 , China.
AD  - Guangdong Food Green Processing and Nutrition Regulation Technologies Research 
      Center , Guangzhou 510650 , China.
FAU - Zhang, Qi
AU  - Zhang Q
AD  - School of Food Science and Engineering , South China University of Technology , 
      Guangzhou 510640 , China.
AD  - Guangdong Food Green Processing and Nutrition Regulation Technologies Research 
      Center , Guangzhou 510650 , China.
FAU - Zhao, Tiantian
AU  - Zhao T
AD  - School of Food Science and Engineering , South China University of Technology , 
      Guangzhou 510640 , China.
AD  - Guangdong Food Green Processing and Nutrition Regulation Technologies Research 
      Center , Guangzhou 510650 , China.
FAU - Liu, Yang
AU  - Liu Y
AD  - School of Food Science and Engineering , South China University of Technology , 
      Guangzhou 510640 , China.
AD  - Guangdong Food Green Processing and Nutrition Regulation Technologies Research 
      Center , Guangzhou 510650 , China.
FAU - Zheng, Lin
AU  - Zheng L
AD  - School of Food Science and Engineering , South China University of Technology , 
      Guangzhou 510640 , China.
FAU - Zhao, Mouming
AU  - Zhao M
AUID- ORCID: 0000-0003-0221-3838
AD  - School of Food Science and Engineering , South China University of Technology , 
      Guangzhou 510640 , China.
AD  - Guangdong Food Green Processing and Nutrition Regulation Technologies Research 
      Center , Guangzhou 510650 , China.
AD  - Beijing Advanced Innovation Center for Food Nutrition and Human Health , Beijing 
      Technology & Business University , Beijing 100048 , China.
LA  - eng
PT  - Journal Article
DEP - 20180928
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 0 (Neuroprotective Agents)
RN  - 0 (Peptides)
RN  - 0 (Reactive Oxygen Species)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - EC 1.11.1.6 (Catalase)
RN  - EC 1.11.1.9 (Glutathione Peroxidase)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Catalase/metabolism
MH  - Female
MH  - Glutathione Peroxidase/metabolism
MH  - Humans
MH  - Juglans/*chemistry
MH  - Male
MH  - Malondialdehyde/metabolism
MH  - Membrane Potential, Mitochondrial/drug effects
MH  - Memory/*drug effects
MH  - Neuroprotective Agents/*administration & dosage/chemistry
MH  - Oxidative Stress/*drug effects
MH  - PC12 Cells
MH  - Peptides/*administration & dosage/chemistry
MH  - Rats
MH  - Reactive Oxygen Species/metabolism
MH  - Sleep Deprivation/*drug therapy/metabolism/physiopathology/psychology
MH  - Superoxide Dismutase/metabolism
OTO - NOTNLM
OT  - PC12 cells
OT  - glutamate
OT  - oxidative injury
OT  - sleep deprivation
OT  - walnut protein hydrolysate
EDAT- 2018/09/19 06:00
MHDA- 2018/10/20 06:00
CRDT- 2018/09/19 06:00
PHST- 2018/09/19 06:00 [pubmed]
PHST- 2018/10/20 06:00 [medline]
PHST- 2018/09/19 06:00 [entrez]
AID - 10.1021/acs.jafc.8b03884 [doi]
PST - ppublish
SO  - J Agric Food Chem. 2018 Oct 10;66(40):10617-10627. doi: 10.1021/acs.jafc.8b03884. 
      Epub 2018 Sep 28.