PMID- 30226543
OWN - NLM
STAT- MEDLINE
DCOM- 20181226
LR  - 20181226
IS  - 1791-2431 (Electronic)
IS  - 1021-335X (Print)
IS  - 1021-335X (Linking)
VI  - 40
IP  - 5
DP  - 2018 Nov
TI  - Characterization of selective exosomal microRNA expression profile derived from 
      laryngeal squamous cell carcinoma detected by next generation sequencing.
PG  - 2584-2594
LID - 10.3892/or.2018.6672 [doi]
AB  - Exosomes are nanometer‑scale extracellular vesicles derived from almost all types 
      of cells and key signaling mediators between cancer cells and their 
      microenvironment. Certain microRNAs (miRNAs) are selected for exosome packing and 
      exclusion from parental cells, while other miRNAs are selectively retained by 
      cells, suggesting a biological role for these miRNAs in tumor malignant 
      progression. In the present study, we isolated and characterized the exosomes 
      derived from the laryngeal squamous cell carcinoma (LSCC) cell line AMC‑HN‑8 for 
      the first time, and identified a subset of miRNAs enriched in the exosomes 
      compared with parental cells, such as miR‑1246, miR‑1290, miR‑335‑5p, miR‑127‑3p 
      and miR‑122‑5p through small RNA sequencing combined with reverse 
      transcription‑quantitative PCR (RT‑qPCR) analysis. Potential target prediction, 
      Gene Ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) 
      pathway enrichment analysis revealed possible functions associated with these 
      selective exosomal miRNAs. In conclusion, the present study demonstrated that the 
      LSCC cell line AMC‑HN‑8 can release exosomes and cells can selectively pack 
      certain miRNAs into exosomes.
FAU - Huang, Qiang
AU  - Huang Q
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Eye, Ear, Nose, and 
      Throat Hospital, Fudan University, Shanghai 200031, P.R. China.
FAU - Yang, Jiechao
AU  - Yang J
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Eye, Ear, Nose, and 
      Throat Hospital, Fudan University, Shanghai 200031, P.R. China.
FAU - Zheng, Juan
AU  - Zheng J
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Eye, Ear, Nose, and 
      Throat Hospital, Fudan University, Shanghai 200031, P.R. China.
FAU - Hsueh, Chiyao
AU  - Hsueh C
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Eye, Ear, Nose, and 
      Throat Hospital, Fudan University, Shanghai 200031, P.R. China.
FAU - Guo, Yang
AU  - Guo Y
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Eye, Ear, Nose, and 
      Throat Hospital, Fudan University, Shanghai 200031, P.R. China.
FAU - Zhou, Liang
AU  - Zhou L
AD  - Department of Otorhinolaryngology Head and Neck Surgery, Eye, Ear, Nose, and 
      Throat Hospital, Fudan University, Shanghai 200031, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20180824
PL  - Greece
TA  - Oncol Rep
JT  - Oncology reports
JID - 9422756
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Carcinoma, Squamous Cell/*genetics/pathology
MH  - Cell Line, Tumor
MH  - Exosomes/genetics
MH  - Extracellular Vesicles/genetics/metabolism
MH  - Gene Expression Regulation, Neoplastic
MH  - *High-Throughput Nucleotide Sequencing
MH  - Humans
MH  - Laryngeal Neoplasms/*genetics/pathology
MH  - MicroRNAs/*genetics
PMC - PMC6151897
EDAT- 2018/09/19 06:00
MHDA- 2018/12/27 06:00
PMCR- 2018/08/24
CRDT- 2018/09/19 06:00
PHST- 2018/03/08 00:00 [received]
PHST- 2018/08/13 00:00 [accepted]
PHST- 2018/09/19 06:00 [pubmed]
PHST- 2018/12/27 06:00 [medline]
PHST- 2018/09/19 06:00 [entrez]
PHST- 2018/08/24 00:00 [pmc-release]
AID - or-40-05-2584 [pii]
AID - 10.3892/or.2018.6672 [doi]
PST - ppublish
SO  - Oncol Rep. 2018 Nov;40(5):2584-2594. doi: 10.3892/or.2018.6672. Epub 2018 Aug 24.