PMID- 30230526
OWN - NLM
STAT- MEDLINE
DCOM- 20190521
LR  - 20231213
IS  - 1097-0215 (Electronic)
IS  - 0020-7136 (Linking)
VI  - 144
IP  - 7
DP  - 2019 Apr 1
TI  - Direct RIG-I activation in human NK cells induces TRAIL-dependent cytotoxicity 
      toward autologous melanoma cells.
PG  - 1645-1656
LID - 10.1002/ijc.31874 [doi]
AB  - Activation of the innate immune receptor retinoic acid-inducible gene I (RIG-I) 
      by its specific ligand 5'-triphosphate RNA (3pRNA) triggers anti-tumor immunity, 
      which is dependent on natural killer (NK) cell activation and cytokine induction. 
      However, to date, RIG-I expression and the functional consequences of RIG-I 
      activation in NK cells have not been examined. Here, we show for the first time 
      the expression of RIG-I in human NK cells and their activation upon RIG-I ligand 
      (3pRNA) transfection. 3pRNA-activated NK cells killed melanoma cells more 
      efficiently than NK cells activated by type I interferon. Stimulation of RIG-I in 
      NK cells specifically increased the surface expression of membrane-bound 
      TNF-related apoptosis-inducing ligand (TRAIL) on NK cells, while activated NK 
      cell receptors were not affected. RIG-I-induced membrane-bound TRAIL initiated 
      death-receptor-pathway-mediated apoptosis not only in allogeneic but also in 
      autologous human leukocyte antigen (HLA) class I-positive and HLA class 
      I-negative melanoma cells. These results identify the direct activation of RIG-I 
      in NK cells as a novel mechanism for how RIG-I can trigger enhanced NK cell 
      killing of tumor cells, underscoring the potential of RIG-I activation for tumor 
      immunotherapy.
CI  - (c) 2018 UICC.
FAU - Dassler-Plenker, Juliane
AU  - Dassler-Plenker J
AUID- ORCID: 0000-0002-1782-102X
AD  - Institute of Clinical Chemistry and Clinical Pharmacology, University Hospital 
      Bonn, University of Bonn, 53127, Bonn, Germany.
FAU - Paschen, Annette
AU  - Paschen A
AD  - Department of Dermatology, Venereology and Allergology, University Hospital 
      Essen, University of Duisburg-Essen, 45112, Essen, Germany.
FAU - Putschli, Bastian
AU  - Putschli B
AD  - Institute of Clinical Chemistry and Clinical Pharmacology, University Hospital 
      Bonn, University of Bonn, 53127, Bonn, Germany.
FAU - Rattay, Stephanie
AU  - Rattay S
AD  - Institute of Clinical Chemistry and Clinical Pharmacology, University Hospital 
      Bonn, University of Bonn, 53127, Bonn, Germany.
FAU - Schmitz, Saskia
AU  - Schmitz S
AD  - Institute of Clinical Chemistry and Clinical Pharmacology, University Hospital 
      Bonn, University of Bonn, 53127, Bonn, Germany.
FAU - Goldeck, Marion
AU  - Goldeck M
AD  - Institute of Clinical Chemistry and Clinical Pharmacology, University Hospital 
      Bonn, University of Bonn, 53127, Bonn, Germany.
FAU - Bartok, Eva
AU  - Bartok E
AD  - Institute of Clinical Chemistry and Clinical Pharmacology, University Hospital 
      Bonn, University of Bonn, 53127, Bonn, Germany.
FAU - Hartmann, Gunther
AU  - Hartmann G
AD  - Institute of Clinical Chemistry and Clinical Pharmacology, University Hospital 
      Bonn, University of Bonn, 53127, Bonn, Germany.
FAU - Coch, Christoph
AU  - Coch C
AD  - Institute of Clinical Chemistry and Clinical Pharmacology, University Hospital 
      Bonn, University of Bonn, 53127, Bonn, Germany.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190109
PL  - United States
TA  - Int J Cancer
JT  - International journal of cancer
JID - 0042124
RN  - 0 (Ligands)
RN  - 0 (Lysosomal-Associated Membrane Protein 1)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (TNF-Related Apoptosis-Inducing Ligand)
RN  - 0 (TNFSF10 protein, human)
RN  - 63231-63-0 (RNA)
RN  - EC 3.6.1.- (RIGI protein, human)
RN  - EC 3.6.4.13 (DEAD Box Protein 58)
SB  - IM
MH  - Apoptosis
MH  - Coculture Techniques
MH  - Cytotoxicity, Immunologic
MH  - DEAD Box Protein 58/*metabolism
MH  - Humans
MH  - Killer Cells, Natural/*cytology/immunology
MH  - Ligands
MH  - Lysosomal-Associated Membrane Protein 1/metabolism
MH  - Melanoma/*immunology/*therapy
MH  - RNA/genetics/*metabolism
MH  - Receptors, Immunologic
MH  - TNF-Related Apoptosis-Inducing Ligand/*metabolism
MH  - Transfection
MH  - Transplantation, Autologous
MH  - Tumor Cells, Cultured
OTO - NOTNLM
OT  - RIG-I
OT  - TRAIL
OT  - innate immunity
OT  - melanoma
OT  - natural killer (NK) cells
EDAT- 2018/09/20 06:00
MHDA- 2019/05/22 06:00
CRDT- 2018/09/20 06:00
PHST- 2018/03/21 00:00 [received]
PHST- 2018/07/30 00:00 [revised]
PHST- 2018/08/22 00:00 [accepted]
PHST- 2018/09/20 06:00 [pubmed]
PHST- 2019/05/22 06:00 [medline]
PHST- 2018/09/20 06:00 [entrez]
AID - 10.1002/ijc.31874 [doi]
PST - ppublish
SO  - Int J Cancer. 2019 Apr 1;144(7):1645-1656. doi: 10.1002/ijc.31874. Epub 2019 Jan 
      9.