PMID- 30230713 OWN - NLM STAT- MEDLINE DCOM- 20190130 LR - 20211204 IS - 2055-5822 (Electronic) IS - 2055-5822 (Linking) VI - 5 IP - 6 DP - 2018 Dec TI - Acute molecular effects of pressure-controlled intermittent coronary sinus occlusion in patients with advanced heart failure. PG - 1176-1183 LID - 10.1002/ehf2.12354 [doi] AB - AIMS: Cardiac repair has steered clinical attention and remains an unmet need, because available regenerative therapies lack robust mechanistic evidence. Pressure-controlled intermittent coronary sinus occlusion (PICSO), known to induce angiogenetic and vasoactive molecules as well as to reduce regional ischemia, may activate endogenous regenerative processes in failing myocardium. We aimed to investigate the effects of PICSO in patients with advanced heart failure undergoing cardiac resynchronization therapy. METHODS AND RESULTS: Eight out of 32 patients were treated with PICSO, and the remainder served as controls. After electrode testing including left ventricular leads, PICSO was performed for 20 min. To test immediate molecular responses, in both patient groups, coronary venous blood samples were taken at baseline and after 20 min, the time required for the intervention. Sera were tested for microRNAs and growth factors. To test the ability of up-regulated soluble factors on cell proliferation and expression of transcription factors [e.g. Kruppel-like factor 4 (KLF-4)], sera were co-cultured with human cardiomyocytes and fibroblasts. As compared with controls, significant differential expression (differences between pre-values and post-values in relation to both patient cohorts) of microRNA patterns associated with cardiac development was observed with PICSO. Importantly, miR-143 (P < 0.048) and miR-145 (P < 0,047) increased, both targeting a network of transcription factors (including KLF-4) that promote differentiation and repress proliferation of vascular smooth muscle cells. Additionally, an increase of miR-19b (P < 0.019) known to alleviate endothelial cell apoptosis was found, whereas disadvantageous miR-320b (P < 0.023) suspect to impair expression of c-myc, normally provoking cell cycle re-entry in post-mitotic myocytes and miR-25 (P < 0.023), decreased, a target of anti-miR application to improve contractility in the failing heart. Co-cultured post-PICSO sera significantly increased cellular proliferation both in fibroblasts (P < 0.001) and adult cardiomycytes (P < 0.004) sampled from a transplant recipient as compared with controls. Adult cardiomyocytes showed a seven-fold increase of the transcription factor KLF-4 protein when co-cultured with treated sera as compared with controls. CONCLUSIONS: Here, we show for the first time that PICSO, a trans-coronary sinus catheter intervention, is associated with an increase in morphogens secreted into cardiac veins, normally present during cardiac development, and a significant induction of cell proliferation. Present findings support the notion that epigenetic modifications, that is, haemodynamic stimuli on venous vascular cells, may reverse myocardial deterioration. Further investigations are needed to decipher the maze of complex interacting molecular pathways in failing myocardium and the potential role of PICSO to reinitiate developmental processes to prevent further myocardial decay eventually reaching clinical significance. CI - (c) 2018 The Authors. ESC Heart Failure published by John Wiley & Sons Ltd on behalf of the European Society of Cardiology. FAU - Mohl, Werner AU - Mohl W AD - Department of Cardiac Surgery, Medical University of Vienna, Vienna, Austria. FAU - Spitzer, Ernest AU - Spitzer E AD - Department of Cardiology, Thoraxcenter, Erasmus University Medical Center, Rotterdam, The Netherlands. FAU - Mader, Robert M AU - Mader RM AD - Department of Medicine I, Comprehensive Cancer Center of the Medical University of Vienna, Vienna, Austria. FAU - Wagh, Vilas AU - Wagh V AD - Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA. FAU - Nguemo, Filomain AU - Nguemo F AD - Center of Physiology and Pathophysiology, Institute of Neurophysiology, University of Cologne, Cologne, Germany. FAU - Milasinovic, Dejan AU - Milasinovic D AD - Department of Cardiology, Clinical Center of Serbia, Belgrade, Serbia. FAU - Jusic, Alem AU - Jusic A AD - Department of Cardiac Surgery, Medical University of Vienna, Vienna, Austria. FAU - Khazen, Cesar AU - Khazen C AD - Department of Cardiac Surgery, Medical University of Vienna, Vienna, Austria. FAU - Szodorai, Edit AU - Szodorai E AD - Department of Molecular Neurosciences, Center for Brain Research, Medical University Vienna, Vienna, Austria. FAU - Birkenberg, Beatrice AU - Birkenberg B AD - Department of Anesthesiology and General Intensive Care, Medical University of Vienna, Vienna, Austria. FAU - Lubec, Gert AU - Lubec G AD - Department of Pharmaceutical Chemistry Faculty of Life Sciences, University of Vienna, Vienna, Austria. FAU - Hescheler, Juergen AU - Hescheler J AD - Center of Physiology and Pathophysiology, Institute of Neurophysiology, University of Cologne, Cologne, Germany. FAU - Serruys, Patrick W AU - Serruys PW AD - International Centre for Circulatory Health, NHLI, Imperial College London, London, UK. LA - eng GR - P13274-Med/Austrian Science Fund FWF/Austria PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20180919 PL - England TA - ESC Heart Fail JT - ESC heart failure JID - 101669191 RN - 0 (Biomarkers) RN - 0 (KLF4 protein, human) RN - 0 (Kruppel-Like Factor 4) SB - IM MH - Aged MH - Balloon Occlusion/*methods MH - Biomarkers/blood MH - Cardiac Catheterization/*methods MH - Coronary Circulation/*physiology MH - Coronary Sinus/*physiopathology MH - Coronary Vessels/*physiopathology MH - Female MH - Heart Failure/blood/physiopathology/*therapy MH - Humans MH - Kruppel-Like Factor 4 MH - Male MH - Middle Aged MH - Pressure PMC - PMC6301157 OTO - NOTNLM OT - Cardiac regeneration OT - Embryonic recall OT - Heart failure OT - PICSO EDAT- 2018/09/20 06:00 MHDA- 2019/01/31 06:00 PMCR- 2018/09/19 CRDT- 2018/09/20 06:00 PHST- 2018/06/10 00:00 [received] PHST- 2018/07/20 00:00 [revised] PHST- 2018/08/08 00:00 [accepted] PHST- 2018/09/20 06:00 [pubmed] PHST- 2019/01/31 06:00 [medline] PHST- 2018/09/20 06:00 [entrez] PHST- 2018/09/19 00:00 [pmc-release] AID - EHF212354 [pii] AID - 10.1002/ehf2.12354 [doi] PST - ppublish SO - ESC Heart Fail. 2018 Dec;5(6):1176-1183. doi: 10.1002/ehf2.12354. Epub 2018 Sep 19.