PMID- 30232360
OWN - NLM
STAT- MEDLINE
DCOM- 20191023
LR  - 20191023
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 8
IP  - 1
DP  - 2018 Sep 19
TI  - Mutagen-induced phytotoxicity in maize seed germination is dependent on ROS 
      scavenging capacity.
PG  - 14078
LID - 10.1038/s41598-018-32271-y [doi]
LID - 14078
AB  - Ethidium bromide (EB) and acridine orange (AO) bind to nucleic acids and are thus 
      considered as potential mutagens. In this study, the effects of EB and AO on the 
      germination behaviours of white, yellow, red, and purple maize seeds were 
      investigated. The results indicate that low concentrations of EB (50 mug mL(-1)) 
      and AO (500 mug mL(-1)) promote germination, particularly for the white and yellow 
      seeds. However, high concentrations of EB (0.5 mg mL(-1)) and AO (5 mg mL(-1)) 
      significantly inhibit germination, with the level of inhibition decreasing in the 
      following order: white > yellow > red > purple. In addition, EB and AO induce 
      H(2)O(2) production in a concentration-dependent manner. The effects of these 
      mutagens on seed germination were partly reversed by dimethyl thiourea, a 
      scavenger of reactive oxygen species (ROS), and diphenylene iodonium (DPI), an 
      inhibitor of NADPH oxidase, while the effects were enhanced by treatment with 
      H(2)O(2) and 3-amino-1,2,4-triazole, a specific inhibitor of catalase. In 
      addition, AO and EB profoundly increased NADPH oxidase activities in germinating 
      seeds. The treatment of seeds with EB and AO did not affect the growth or drought 
      tolerance of the resultant seedlings. The findings suggest that the mechanism of 
      mutagen toxicity is related to the induction of ROS production.
FAU - Zhang, Yifei
AU  - Zhang Y
AD  - Heilongjiang Provincial Key Laboratory of Modern Agricultural Cultivation and 
      Crop Germplasm Improvement, Department of Agronomy, Heilongjiang Bayi 
      Agricultural University, Daqing, 163319, China.
FAU - Shi, Haojie
AU  - Shi H
AD  - School of Agricultural and Food Sciences, Zhejiang Agriculture and Forest 
      University, Hangzhou, PR China.
FAU - Deng, Benliang
AU  - Deng B
AUID- ORCID: 0000-0002-7301-7140
AD  - Heilongjiang Provincial Key Laboratory of Modern Agricultural Cultivation and 
      Crop Germplasm Improvement, Department of Agronomy, Heilongjiang Bayi 
      Agricultural University, Daqing, 163319, China. benliangdeng@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180919
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Mutagens)
RN  - 0 (Reactive Oxygen Species)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 1.6.3.- (NADPH Oxidases)
RN  - EN464416SI (Ethidium)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange/toxicity
MH  - Dose-Response Relationship, Drug
MH  - Droughts
MH  - Ethidium/toxicity
MH  - Gene Expression Regulation, Plant/drug effects
MH  - Germination/*drug effects
MH  - Hydrogen Peroxide/metabolism
MH  - Mutagens/*toxicity
MH  - NADPH Oxidases/metabolism
MH  - Reactive Oxygen Species/*metabolism
MH  - Stress, Physiological/drug effects
MH  - Zea mays/drug effects/*physiology
PMC - PMC6145914
COIS- The authors declare no competing interests.
EDAT- 2018/09/21 06:00
MHDA- 2019/10/24 06:00
PMCR- 2018/09/19
CRDT- 2018/09/21 06:00
PHST- 2018/04/09 00:00 [received]
PHST- 2018/09/05 00:00 [accepted]
PHST- 2018/09/21 06:00 [entrez]
PHST- 2018/09/21 06:00 [pubmed]
PHST- 2019/10/24 06:00 [medline]
PHST- 2018/09/19 00:00 [pmc-release]
AID - 10.1038/s41598-018-32271-y [pii]
AID - 32271 [pii]
AID - 10.1038/s41598-018-32271-y [doi]
PST - epublish
SO  - Sci Rep. 2018 Sep 19;8(1):14078. doi: 10.1038/s41598-018-32271-y.