PMID- 30232645
OWN - NLM
STAT- MEDLINE
DCOM- 20190705
LR  - 20200225
IS  - 1435-604X (Electronic)
IS  - 0268-8921 (Linking)
VI  - 34
IP  - 4
DP  - 2019 Jun
TI  - Effect of photobiomodulation on neural differentiation of human umbilical cord 
      mesenchymal stem cells.
PG  - 667-675
LID - 10.1007/s10103-018-2638-y [doi]
AB  - Photobiomodulation therapy (PBMT) can enhance the mesenchymal stem cell (MSC) 
      proliferation, differentiation, and tissue repair and can therefore be used in 
      regenerative medicine. The objective of this study is to investigate the effects 
      of photobiomodulation on the directional neural differentiation of human 
      umbilical cord mesenchymal stem cells (hUC-MSCs) and provide a theoretical basis 
      for neurogenesis. hUC-MSCs were divided into control, inducer, laser, and lasers 
      combined with inducer groups. A 635-nm laser and an 808-nm laser delivering 
      energy densities from 0 to 10 J/cm(2) were used in the study. Normal 
      cerebrospinal fluid (CSF) and injured cerebrospinal fluid (iCSF) were used as 
      inducers. The groups were continuously induced for 3 days. Cellular proliferation 
      was evaluated using MTT. The marker proteins nestin (marker protein of the neural 
      precursor cells), NeuN (marker protein of neuron), and GFAP (glial fibrillary 
      acidic protein, marker proteins of glial cells) were detected by 
      immunofluorescence and western blot. We found that irradiation with 635-nm laser 
      increased cell proliferation, and that with 808 nm laser by itself and combined 
      with cerebrospinal fluid treatment generated significant neuron-like 
      morphological changes in the cells at 72 h. Nestin showed high positive 
      expression at 24 h in the 808 nm group. The expression of GFAP increased in the 
      808-nm combined inducer group at 24 h but decreased at 72 h. The expression of 
      neuN protein increased only at 72 h in both the 808-nm combined inducer group and 
      inducer group. We concluded that 808 nm laser irradiation could help CSF to 
      induce neuronal differentiation of hUC-MSCs in early stage and tend to change to 
      neuron rather than glial cells.
FAU - Chen, Hongli
AU  - Chen H
AD  - School of Electronics and Information Engineering, Tianjin Polytechnic 
      University, Tianjin, 300387, China.
AD  - Laboratory of Laser Medicine, Institute of Biomedical Engineering, Chinese 
      Academy of Medical Sciences & Peking Union Medical College, Tianjin, 300192, 
      China.
FAU - Wu, Hongjun
AU  - Wu H
AD  - School of Electronics and Information Engineering, Tianjin Polytechnic 
      University, Tianjin, 300387, China.
FAU - Yin, Huijuan
AU  - Yin H
AD  - Laboratory of Laser Medicine, Institute of Biomedical Engineering, Chinese 
      Academy of Medical Sciences & Peking Union Medical College, Tianjin, 300192, 
      China. yinzi490@163.com.
FAU - Wang, Jinhai
AU  - Wang J
AD  - School of Electronics and Information Engineering, Tianjin Polytechnic 
      University, Tianjin, 300387, China. wangjinhai@tjpu.edu.cn.
FAU - Dong, Huajiang
AU  - Dong H
AD  - Logistics University of People's Armed Police Force, Tianjin, 300309, China.
FAU - Chen, Qianqian
AU  - Chen Q
AD  - Laboratory of Laser Medicine, Institute of Biomedical Engineering, Chinese 
      Academy of Medical Sciences & Peking Union Medical College, Tianjin, 300192, 
      China.
FAU - Li, Yingxin
AU  - Li Y
AD  - Laboratory of Laser Medicine, Institute of Biomedical Engineering, Chinese 
      Academy of Medical Sciences & Peking Union Medical College, Tianjin, 300192, 
      China.
LA  - eng
GR  - No.61705164/the National Natural Science Foundation of China/
GR  - No.81801240/National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20180919
PL  - England
TA  - Lasers Med Sci
JT  - Lasers in medical science
JID - 8611515
RN  - 0 (Antigens, Nuclear)
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Nestin)
RN  - 0 (neuronal nuclear antigen NeuN, human)
SB  - IM
MH  - Antigens, Nuclear/metabolism
MH  - Cell Differentiation/*radiation effects
MH  - Cell Proliferation/radiation effects
MH  - Cell Shape/radiation effects
MH  - Cells, Cultured
MH  - Glial Fibrillary Acidic Protein/metabolism
MH  - Humans
MH  - Immunophenotyping
MH  - *Low-Level Light Therapy
MH  - Mesenchymal Stem Cells/*cytology/*radiation effects
MH  - Nerve Tissue Proteins/metabolism
MH  - Nestin/metabolism
MH  - Neurogenesis/radiation effects
MH  - Neurons/*cytology/*radiation effects
MH  - Umbilical Cord/*cytology
OTO - NOTNLM
OT  - Cerebrospinal fluid
OT  - Low-level laser
OT  - Mesenchymal stem cells
OT  - Neural differentiation
OT  - Photobiomodulation
EDAT- 2018/09/21 06:00
MHDA- 2019/07/06 06:00
CRDT- 2018/09/21 06:00
PHST- 2018/07/24 00:00 [received]
PHST- 2018/09/11 00:00 [accepted]
PHST- 2018/09/21 06:00 [pubmed]
PHST- 2019/07/06 06:00 [medline]
PHST- 2018/09/21 06:00 [entrez]
AID - 10.1007/s10103-018-2638-y [pii]
AID - 10.1007/s10103-018-2638-y [doi]
PST - ppublish
SO  - Lasers Med Sci. 2019 Jun;34(4):667-675. doi: 10.1007/s10103-018-2638-y. Epub 2018 
      Sep 19.