PMID- 30236758
OWN - NLM
STAT- MEDLINE
DCOM- 20190520
LR  - 20190520
IS  - 1879-0003 (Electronic)
IS  - 0141-8130 (Linking)
VI  - 125
DP  - 2019 Mar 15
TI  - A cold-water soluble polysaccharide isolated from Grifola frondosa induces the 
      apoptosis of HepG2 cells through mitochondrial passway.
PG  - 1232-1241
LID - S0141-8130(18)33410-X [pii]
LID - 10.1016/j.ijbiomac.2018.09.098 [doi]
AB  - Grifola frondosa is a widely eaten and medicinal fungus. In this study, we 
      extracted a cold-water-soluble polysaccharide from Grifola frondosa (cGFP) and 
      investigated its effects on the proliferation and apoptosis of human hepatoma 
      HepG2 cells. MTT assay showed that cGFP induced apoptosis of HepG2 cells in a 
      dose-dependent manner. Flow cytometry analysis showed that cGFP induced apoptosis 
      in HepG2 cells through S phase arrest. The distribution of cells at different 
      apoptotic stages was determined by Annexin V-FITC and Propidium Iodide (PI) 
      staining. Scanning electron microscopy (SEM) results indicated that cGFP induced 
      typical apoptotic morphological features in HepG2. Mitochondrial membrane 
      potential was reduced according to the screening of JC-1 staining. And western 
      blot analysis of Bax, Bcl-2, cytochrome C (Cyto-c), caspase-3, and caspase-9 
      further demonstrated that the cGFP-induced apoptosis effect functioned through 
      the mitochondrial pathway. Further analysis by qRT-PCR showed that Bax expression 
      increased and Bcl-2 expression decreased. These findings suggested that cGFP 
      could inhibit the proliferation of HepG2 cells and induce apoptosis mainly 
      through the intrinsic activation mitochondrial pathway.
CI  - Copyright (c) 2018. Published by Elsevier B.V.
FAU - Chen, Pei
AU  - Chen P
AD  - State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Food 
      Nutrition and Safety, Ministry of Education of China, College of Food Engineering 
      and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, 
      PR China.
FAU - Liu, Hui-Ping
AU  - Liu HP
AD  - State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Food 
      Nutrition and Safety, Ministry of Education of China, College of Food Engineering 
      and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, 
      PR China. Electronic address: liuhuiping@tust.edu.cn.
FAU - Ji, Hai-Hu
AU  - Ji HH
AD  - State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Food 
      Nutrition and Safety, Ministry of Education of China, College of Food Engineering 
      and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, 
      PR China.
FAU - Sun, Na-Xin
AU  - Sun NX
AD  - State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Food 
      Nutrition and Safety, Ministry of Education of China, College of Food Engineering 
      and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, 
      PR China.
FAU - Feng, Ying-Ying
AU  - Feng YY
AD  - State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Food 
      Nutrition and Safety, Ministry of Education of China, College of Food Engineering 
      and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, 
      PR China.
LA  - eng
PT  - Journal Article
DEP - 20180917
PL  - Netherlands
TA  - Int J Biol Macromol
JT  - International journal of biological macromolecules
JID - 7909578
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Fungal Polysaccharides)
RN  - 0 (Monosaccharides)
SB  - IM
MH  - Antineoplastic Agents/pharmacology
MH  - Apoptosis/*drug effects
MH  - Cell Cycle/drug effects
MH  - Cell Proliferation/drug effects
MH  - Cell Survival/drug effects
MH  - Fungal Polysaccharides/chemistry/isolation & purification/*pharmacology
MH  - Gene Expression
MH  - Genes, Reporter
MH  - Grifola/*chemistry
MH  - Hep G2 Cells/ultrastructure
MH  - Humans
MH  - Membrane Potential, Mitochondrial
MH  - Mitochondria/*drug effects/*metabolism
MH  - Monosaccharides/chemistry
MH  - Signal Transduction/*drug effects
MH  - Spectrum Analysis
OTO - NOTNLM
OT  - Cell apoptosis
OT  - Cold-water-soluble polysaccharide
OT  - Grifola frondosa polysaccharide
OT  - Mitochondrial pathway
EDAT- 2018/09/22 06:00
MHDA- 2019/05/21 06:00
CRDT- 2018/09/22 06:00
PHST- 2018/07/06 00:00 [received]
PHST- 2018/09/14 00:00 [revised]
PHST- 2018/09/16 00:00 [accepted]
PHST- 2018/09/22 06:00 [pubmed]
PHST- 2019/05/21 06:00 [medline]
PHST- 2018/09/22 06:00 [entrez]
AID - S0141-8130(18)33410-X [pii]
AID - 10.1016/j.ijbiomac.2018.09.098 [doi]
PST - ppublish
SO  - Int J Biol Macromol. 2019 Mar 15;125:1232-1241. doi: 
      10.1016/j.ijbiomac.2018.09.098. Epub 2018 Sep 17.