PMID- 30237316
OWN - NLM
STAT- MEDLINE
DCOM- 20190916
LR  - 20191210
IS  - 1470-8736 (Electronic)
IS  - 0143-5221 (Linking)
VI  - 132
IP  - 21
DP  - 2018 Nov 15
TI  - The down-regulation of hsa_circ_0012919, the sponge for miR-125a-3p, contributes 
      to DNA methylation of CD11a and CD70 in CD4(+) T cells of systemic lupus 
      erythematous.
PG  - 2285-2298
LID - 10.1042/CS20180403 [doi]
AB  - BACKGROUND: Systemic lupus erythematous (SLE) is an autoimmune disease 
      characterized by the production of autoantibodies directed against various 
      autoantigens. But the expression profiles and functions of circular RNAs 
      (circRNAs) in SLE are still scarce. OBJECTIVES: To explore the roles of circRNA 
      in SLE and its potential diagnostic potential in SLE. METHODS: SLE patients and 
      healthy control subjects were recruited. CD4(+) T cells were isolated, circRNA 
      microarray analysis were used to screen for circRNA candidate in CD4(+) T cells. 
      Expression of DNMT1, CD11a and CD70, and methylation level of CD11a and CD70 were 
      detected after transfecting hsa_circ_0012919-targetted siRNA. The network 
      analysis of hsa_circ_0012919 was used by bioinformatics. Luciferase reporter 
      assay and fluorescence in situ hybridization (FISH) assay were used for screening 
      for which miRNAs could bind with hsa_circ_0012919. RESULTS: Twelve circRNAs were 
      up-regulated and two circRNAs were down-regulated in SLE patients group after 
      circRNA microarray analysis. Hsa_circ_0012919 was further confirmed to be 
      significantly different between healthy control and SLE patients (P<0.05) and 
      associated with SLE characters (P<0.05). Down-regulation of hsa_circ_0012919 (i) 
      increased the expression of DNMT1 and reduced the expression of CD70, CD11a, (ii) 
      reversed the DNA hypomethylation of CD11a and CD70 in CD4(+) T cells of SLE, but 
      it could be reversed by down-regulation of DNMT1. Hsa_circ_0012919 regulated 
      KLF13 and RANTES by miR-125a Conclusion: Hsa_circ_0012919 could be regarded as a 
      biomarker for SLE and hsa_circ_0012919 was the competitive endogenous RNA (ceRNA) 
      for miR-125a-3p.
CI  - (c) 2018 The Author(s). Published by Portland Press Limited on behalf of the 
      Biochemical Society.
FAU - Zhang, Chengzhong
AU  - Zhang C
AD  - Department of Dermatology, Shanghai General Hospital, Shanghai Jiaotong 
      University School of Medicine, Wujin Road 85, Hongkou District, Shanghai 200080, 
      China.
FAU - Wang, Xin
AU  - Wang X
AD  - Department of Dermatology, Shanghai General Hospital, Shanghai Jiaotong 
      University School of Medicine, Wujin Road 85, Hongkou District, Shanghai 200080, 
      China.
FAU - Chen, Yue
AU  - Chen Y
AD  - Department of Dermatology, Shanghai General Hospital, Shanghai Jiaotong 
      University School of Medicine, Wujin Road 85, Hongkou District, Shanghai 200080, 
      China.
FAU - Wu, Zhouwei
AU  - Wu Z
AD  - Department of Dermatology, Shanghai General Hospital, Shanghai Jiaotong 
      University School of Medicine, Wujin Road 85, Hongkou District, Shanghai 200080, 
      China.
FAU - Zhang, Chao
AU  - Zhang C
AD  - Department of Dermatology, Minhang Hospital, Fudan University, Xinsong Road 170, 
      Minhang District, Shanghai 201199, China charleszhang1980@126.com 
      shiyipifu@163.com.
AD  - Institute of Fudan-Minhang Academic Health System, Minhang Hospital, Fudan 
      University, Xinsong Road 170, Minhang District, Shanghai 201199, China.
FAU - Shi, Weimin
AU  - Shi W
AUID- ORCID: 0000-0002-0401-1175
AD  - Department of Dermatology, Shanghai General Hospital, Shanghai Jiaotong 
      University School of Medicine, Wujin Road 85, Hongkou District, Shanghai 200080, 
      China charleszhang1980@126.com shiyipifu@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20181102
PL  - England
TA  - Clin Sci (Lond)
JT  - Clinical science (London, England : 1979)
JID - 7905731
RN  - 0 (CCL5 protein, human)
RN  - 0 (CD11a Antigen)
RN  - 0 (CD27 Ligand)
RN  - 0 (CD70 protein, human)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Chemokine CCL5)
RN  - 0 (Genetic Markers)
RN  - 0 (KLF13 protein, human)
RN  - 0 (Kruppel-Like Transcription Factors)
RN  - 0 (MIRN125 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Circular)
RN  - 0 (Repressor Proteins)
RN  - 63231-63-0 (RNA)
RN  - EC 2.1.1.37 (DNA (Cytosine-5-)-Methyltransferase 1)
RN  - EC 2.1.1.37 (DNMT1 protein, human)
SB  - IM
CIN - Clin Sci (Lond). 2019 May 7;133(9):1049-1052. PMID: 31064795
MH  - Adult
MH  - CD11a Antigen/*genetics/immunology/metabolism
MH  - CD27 Ligand/*genetics/immunology/metabolism
MH  - CD4-Positive T-Lymphocytes/immunology/*metabolism
MH  - Case-Control Studies
MH  - Cell Cycle Proteins/genetics/metabolism
MH  - Cells, Cultured
MH  - Chemokine CCL5/genetics/metabolism
MH  - DNA (Cytosine-5-)-Methyltransferase 1/genetics/metabolism
MH  - *DNA Methylation
MH  - Female
MH  - Gene Expression Profiling/methods
MH  - Gene Regulatory Networks
MH  - Genetic Markers
MH  - Humans
MH  - Kruppel-Like Transcription Factors/genetics/metabolism
MH  - Lupus Erythematosus, Systemic/*genetics/immunology/metabolism
MH  - MicroRNAs/*genetics/metabolism
MH  - Oligonucleotide Array Sequence Analysis
MH  - RNA/*genetics/metabolism
MH  - RNA, Circular
MH  - Repressor Proteins/genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
OTO - NOTNLM
OT  - Biomarker
OT  - System lupus erythematous
OT  - hsa_circ_0012919
OT  - miR-125a-3p
EDAT- 2018/09/22 06:00
MHDA- 2019/09/17 06:00
CRDT- 2018/09/22 06:00
PHST- 2018/05/13 00:00 [received]
PHST- 2018/09/18 00:00 [revised]
PHST- 2018/09/19 00:00 [accepted]
PHST- 2018/09/22 06:00 [pubmed]
PHST- 2019/09/17 06:00 [medline]
PHST- 2018/09/22 06:00 [entrez]
AID - CS20180403 [pii]
AID - 10.1042/CS20180403 [doi]
PST - epublish
SO  - Clin Sci (Lond). 2018 Nov 2;132(21):2285-2298. doi: 10.1042/CS20180403. Print 
      2018 Nov 15.