PMID- 30238225
OWN - NLM
STAT- MEDLINE
DCOM- 20190328
LR  - 20200309
IS  - 2092-9293 (Electronic)
IS  - 1976-9571 (Linking)
VI  - 41
IP  - 1
DP  - 2019 Jan
TI  - Deciphering genome-wide WRKY gene family of Triticum aestivum L. and their 
      functional role in response to Abiotic stress.
PG  - 79-94
LID - 10.1007/s13258-018-0742-9 [doi]
AB  - WRKY transcription factors (TFs) act in regulating plant growth and development 
      as well as in response to different stress. Some earlier studies done by 
      individual researchers reported different wheat WRKY TFs. Although, the recently 
      released wheat genome has opened an avenue to investigate wheat WRKYs (TaWRKY) 
      TFs. Prime objective of this study to performed genome-wide classifications of 
      TaWRKYs and their functional annotation. The classification of 107 individual 
      identified characterized sequences of TaWRKY (IICS-TaWRKY) and 160 
      uncharacterized draft sequences of TaWRKY (UDS-TaWRKY), along with their gene 
      structures and motifs analysis was performed. Along with comparative sequence 
      analysis and microarray analysis was performed to mimic out TaWRKYs functions in 
      response to different abiotic stresses, accompanied by in-vitro validation. The 
      comparative phylogenetic analysis and estimation of Ka/Ks ratio with Triticum 
      urartu, illustrate group based clasifications of TaWRKYs and evolutionary 
      divergences. Furthermore, motif-based and protein-DNA interaction analysis of 
      TaWRKYs helps to identify, their putative function in target DNA recognition 
      sites. Subsequently, results of microarray and comparative sequence analysis 
      provides the evidence of TaWRKYs involved in heat and/or drought stress. Further, 
      in-vitro results validates that TaWRKY014, TaWRKY090 are found to participate in 
      response of drought stress, whereas TaWRKY008, TaWRKY122, and WRKY45 are involved 
      in response of heat and drought stress. These findings can be utilized in 
      developing novel heat and drought-tolerant wheat cultivars using marker-assisted 
      breeding and transgenic development.
FAU - Gupta, Saurabh
AU  - Gupta S
AD  - Department of Applied Sciences, Indian Institute of Information Technology, 
      Deoghat, Jhalwa, Allahabad, 211015, India.
AD  - AgriGenome Labs Pvt. Ltd., Hyderabad, 500078, India.
FAU - Mishra, Vinod Kumar
AU  - Mishra VK
AD  - Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, 221005, 
      India.
FAU - Kumari, Sunita
AU  - Kumari S
AD  - Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, 221005, 
      India.
FAU - Raavi
AU  - Raavi
AD  - Molecular Biology, Cell Biology and Biochemistry Program, Boston University, 
      Boston, 02215, USA.
FAU - Chand, Ramesh
AU  - Chand R
AD  - Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, 221005, 
      India.
FAU - Varadwaj, Pritish Kumar
AU  - Varadwaj PK
AD  - Department of Applied Sciences, Indian Institute of Information Technology, 
      Deoghat, Jhalwa, Allahabad, 211015, India. pritish@iiita.ac.in.
LA  - eng
PT  - Journal Article
DEP - 20180920
PL  - Korea (South)
TA  - Genes Genomics
JT  - Genes & genomics
JID - 101481027
RN  - 0 (Plant Proteins)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Gene Expression Regulation, Plant
MH  - *Multigene Family
MH  - Plant Proteins/*genetics/metabolism
MH  - *Stress, Physiological
MH  - Transcription Factors/*genetics/metabolism
MH  - Triticum/*genetics
OTO - NOTNLM
OT  - Drought stress
OT  - Gene amplification
OT  - Heat stress
OT  - WRKY TFs
OT  - Wheat
EDAT- 2018/09/22 06:00
MHDA- 2019/03/29 06:00
CRDT- 2018/09/22 06:00
PHST- 2018/06/18 00:00 [received]
PHST- 2018/09/11 00:00 [accepted]
PHST- 2018/09/22 06:00 [pubmed]
PHST- 2019/03/29 06:00 [medline]
PHST- 2018/09/22 06:00 [entrez]
AID - 10.1007/s13258-018-0742-9 [pii]
AID - 10.1007/s13258-018-0742-9 [doi]
PST - ppublish
SO  - Genes Genomics. 2019 Jan;41(1):79-94. doi: 10.1007/s13258-018-0742-9. Epub 2018 
      Sep 20.