PMID- 30238979
OWN - NLM
STAT- MEDLINE
DCOM- 20200316
LR  - 20200316
IS  - 1097-4652 (Electronic)
IS  - 0021-9541 (Linking)
VI  - 234
IP  - 4
DP  - 2019 Apr
TI  - Exercise rescues the immune response fine-tuned impaired by peroxisome 
      proliferator-activated receptors gamma deletion in macrophages.
PG  - 5241-5251
LID - 10.1002/jcp.27333 [doi]
AB  - BACKGROUND: Exercise is a powerful tool for prevention and treatment of many 
      conditions related to the cardiovascular system and also chronic low-grade 
      inflammation. Peroxisome proliferator-activated receptors gamma (PPARgamma) exerts an 
      import role on the regulation of metabolic profile and subsequent inflammatory 
      response, especially in macrophages. PURPOSE: To investigate the effects of 
      8-week moderate-exercise training on metabolic and inflammatory parameters in 
      mice with PPARgamma deficiency in myeloid cells. METHODS: Twelve-week old mice 
      bearing PPARgamma deletion exclusively in myeloid cells (PPARgammalox/lox Lys Cre (-/+) , 
      knockout [KO]) and littermate controls (PPARgammalox/lox Lys Cre (-/-) , wild type 
      [WT]) were submitted to 8-week exercise training (treadmill running at moderate 
      intensity, 5 days/week). Animals were evaluated for food intake, glucose 
      homeostasis, serum metabolites, adipose tissue and peritoneal macrophage 
      inflammation, and basal and stimulated cytokine secretion. RESULTS: Exercise 
      protocol did not improve glucose metabolism or adiponectin concentrations in 
      serum of KO mice. Moreover, the absence of PPARgamma in macrophages exacerbated the 
      proinflammatory profile in sedentary mice. Peritoneal cultured cells had higher 
      tumor necrosis factor-alpha (TNF-alpha) secretion in nonstimulated and lipopolysaccharide 
      (LPS)-stimulated conditions and higher Toll-4 receptor (TLR4) gene expression 
      under LPS stimulus. Trained mice showed reduced TNF-alpha content in adipose tissue 
      independently of the genotype. M2 polarization ability was impaired in KO 
      peritoneal macrophages after exercise training, while adipose tissue-associated 
      macrophages did not present any effect by PPARgamma ablation. CONCLUSION: Overall, 
      PPARgamma seems necessary to maintain macrophages appropriate response to 
      inflammatory stimulus and macrophage polarization, affecting also whole body 
      lipid metabolism and adiponectin profile. Exercise training showed as an 
      efficient mechanism to restore the immune response impaired by PPARgamma deletion in 
      macrophages.
CI  - (c) 2018 Wiley Periodicals, Inc.
FAU - Silveira, Loreana Sanches
AU  - Silveira LS
AUID- ORCID: 0000-0003-4429-0153
AD  - Department of Physical Education, Exercise and Immunometabolism Research Group, 
      Post-Graduation Program in Movement Sciences, Sao Paulo State University (UNESP), 
      Sao Paulo, Brazil.
AD  - Department of Cell and Developmental Biology, Institute of Biomedical Sciences, 
      University of Sao Paulo (USP), Sao Paulo, Brazil.
FAU - Batatinha, Helena Angelica Pereira
AU  - Batatinha HAP
AD  - Department of Cell and Developmental Biology, Institute of Biomedical Sciences, 
      University of Sao Paulo (USP), Sao Paulo, Brazil.
FAU - Castoldi, Angela
AU  - Castoldi A
AD  - Department of Immunology, Institute of Biomedical Sciences, University of Sao 
      Paulo, Sao Paulo, Brazil.
FAU - Camara, Niels Olsen Saraiva
AU  - Camara NOS
AD  - Department of Immunology, Institute of Biomedical Sciences, University of Sao 
      Paulo, Sao Paulo, Brazil.
FAU - Festuccia, Willian T
AU  - Festuccia WT
AD  - Department of Physiology and Biophysics, Institute of Biomedical Sciences, 
      University of Sao Paulo, Sao Paulo, Brazil.
FAU - Souza, Camila Oliveira
AU  - Souza CO
AD  - Department of Cell and Developmental Biology, Institute of Biomedical Sciences, 
      University of Sao Paulo (USP), Sao Paulo, Brazil.
FAU - Rosa Neto, Jose Cesar
AU  - Rosa Neto JC
AD  - Department of Cell and Developmental Biology, Institute of Biomedical Sciences, 
      University of Sao Paulo (USP), Sao Paulo, Brazil.
FAU - Lira, Fabio Santos
AU  - Lira FS
AD  - Department of Physical Education, Exercise and Immunometabolism Research Group, 
      Post-Graduation Program in Movement Sciences, Sao Paulo State University (UNESP), 
      Sao Paulo, Brazil.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180921
PL  - United States
TA  - J Cell Physiol
JT  - Journal of cellular physiology
JID - 0050222
RN  - 0 (Adiponectin)
RN  - 0 (Adipoq protein, mouse)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Lipids)
RN  - 0 (PPAR gamma)
RN  - 0 (Pparg protein, mouse)
SB  - IM
MH  - Adiponectin/blood
MH  - Adipose Tissue/metabolism
MH  - Animals
MH  - *Cell Plasticity
MH  - Cells, Cultured
MH  - *Energy Metabolism
MH  - Gene Deletion
MH  - Inflammation Mediators/*metabolism
MH  - Lipids/blood
MH  - Macrophages, Peritoneal/immunology/*metabolism
MH  - Male
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - PPAR gamma/*deficiency/genetics
MH  - Phenotype
MH  - Physical Conditioning, Animal/*methods
MH  - Physical Endurance
MH  - Time Factors
OTO - NOTNLM
OT  - aerobic training
OT  - cytokines
OT  - energetic metabolism
OT  - transcriptional factor
EDAT- 2018/09/22 06:00
MHDA- 2020/03/17 06:00
CRDT- 2018/09/22 06:00
PHST- 2018/03/13 00:00 [received]
PHST- 2018/08/10 00:00 [accepted]
PHST- 2018/09/22 06:00 [pubmed]
PHST- 2020/03/17 06:00 [medline]
PHST- 2018/09/22 06:00 [entrez]
AID - 10.1002/jcp.27333 [doi]
PST - ppublish
SO  - J Cell Physiol. 2019 Apr;234(4):5241-5251. doi: 10.1002/jcp.27333. Epub 2018 Sep 
      21.