PMID- 30241074 OWN - NLM STAT- MEDLINE DCOM- 20181017 LR - 20181017 IS - 1873-376X (Electronic) IS - 1570-0232 (Linking) VI - 1097-1098 DP - 2018 Oct 15 TI - Mechanism of YLTZ on glycolipid metabolism based on UPLC/TOF/MS metabolomics. PG - 128-141 LID - S1570-0232(18)30839-0 [pii] LID - 10.1016/j.jchromb.2018.08.023 [doi] AB - Type 2 diabetes mellitus (T2DM) is a metabolic disease characterized by dysfunction of glycolipid metabolism. YLTZ is used to treat hyperlipidemia, yet its hypolipidemic and hypoglycemic mechanism on T2DM are unknown. Thus, UPLC/TOF/MS was applied in this study to identify the potential bio-markers, and deduce the possible metabolic pathways. According to bio-indexes, the increased blood lipid levels, including TC, TG, LDL and FA, and the decreased HDL, the elevated glucose, reduced insulin level and impaired OGTT were observed in diabetic rat model. While YLTZ can decrease the lipid levels and glucose content, as well as increased insulin standards and improve OGTT. After data from UPLC/TOF/MS processed, 17 metabolites were obtained, including phospholipids (LPCs, PCs and PGP (18:1)), beta-oxidation production (HAA, VAG and CNE) and precursors (THA), bile acid (CA, CDCA and IDCA), hydrolysate of TG (MG (22:4)), glycometabolism (G6P), cholesterol-driven synthetics (ADO) and production of arachidonate acid (THETA). As a result, YLTZ was able to reduce LPCs, PCs, PGP (18:1), HAA, VAG, CNE, CA, ADO and THETA, as well as enhance MG (22:4) and G6P. After analyzing results, several metabolic pathways were deduced, which containing, cholesterol synthesis and elimination, FA beta-oxidation, TG hydrolysis, phospholipids synthesis, glycolysis, gluconeogenesis and inflammation. Consequently, YLTZ performed to prohibit the FA beta-oxidation, synthesis of cholesterol and phospholipids, gluconeogenesis and inflammation level, as well as promote TG hydrolysis, glycolysis and blood circulation. Hence, applying metabonomics in TCM research can uncover its pharmacological edges, elucidating comprehensively that YLTZ has capacity of hypolipidemic, hypoglycemic and promoting blood circulation, matching the effect of removing blood stasis, eliminating phlegm and dampness. CI - Copyright (c) 2018 Elsevier B.V. All rights reserved. FAU - Zhuo, Jun-Cheng AU - Zhuo JC AD - Guangzhou University of Chinese Medicine, Guangzhou 510405, China; Guangdong Province Engineering Technology Research Institute of T.C.M., Guangzhou 510095, China; Guangdong Second Traditional Chinese Medicine Hospital, Guangzhou, Guangdong 510095, China. FAU - Cai, Da-Ke AU - Cai DK AD - Guangdong Province Engineering Technology Research Institute of T.C.M., Guangzhou 510095, China; Guangdong Second Traditional Chinese Medicine Hospital, Guangzhou, Guangdong 510095, China. FAU - Xie, Kai-Feng AU - Xie KF AD - Guangzhou University of Chinese Medicine, Guangzhou 510405, China; Guangdong Province Engineering Technology Research Institute of T.C.M., Guangzhou 510095, China; Guangdong Second Traditional Chinese Medicine Hospital, Guangzhou, Guangdong 510095, China. FAU - Gan, Hai-Ning AU - Gan HN AD - Guangdong Province Engineering Technology Research Institute of T.C.M., Guangzhou 510095, China; Guangdong Second Traditional Chinese Medicine Hospital, Guangzhou, Guangdong 510095, China. FAU - Li, Sha-Sha AU - Li SS AD - Guangdong Provincial Hospital of Chinese Medicine, Guangzhou, China. FAU - Huang, Xue-Jun AU - Huang XJ AD - Guangdong Province Engineering Technology Research Institute of T.C.M., Guangzhou 510095, China; Guangdong Second Traditional Chinese Medicine Hospital, Guangzhou, Guangdong 510095, China. FAU - Huang, Dane AU - Huang D AD - Guangdong Province Engineering Technology Research Institute of T.C.M., Guangzhou 510095, China; Guangdong Second Traditional Chinese Medicine Hospital, Guangzhou, Guangdong 510095, China. FAU - Zhang, Cheng-Zhe AU - Zhang CZ AD - Guangdong Province Engineering Technology Research Institute of T.C.M., Guangzhou 510095, China; Guangdong Second Traditional Chinese Medicine Hospital, Guangzhou, Guangdong 510095, China. FAU - Li, Ru-Yue AU - Li RY AD - Guangzhou University of Chinese Medicine, Guangzhou 510405, China; Guangdong Province Engineering Technology Research Institute of T.C.M., Guangzhou 510095, China; Guangdong Second Traditional Chinese Medicine Hospital, Guangzhou, Guangdong 510095, China. FAU - Chen, Yu-Xing AU - Chen YX AD - Guangdong Province Engineering Technology Research Institute of T.C.M., Guangzhou 510095, China; Guangdong Second Traditional Chinese Medicine Hospital, Guangzhou, Guangdong 510095, China. Electronic address: tcmgdp@126.com. FAU - Zeng, Xiao-Hui AU - Zeng XH AD - Guangdong Province Engineering Technology Research Institute of T.C.M., Guangzhou 510095, China; Guangdong Second Traditional Chinese Medicine Hospital, Guangzhou, Guangdong 510095, China. Electronic address: simao37@163.com. LA - eng PT - Journal Article DEP - 20180824 PL - Netherlands TA - J Chromatogr B Analyt Technol Biomed Life Sci JT - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JID - 101139554 RN - 0 (Biomarkers) RN - 0 (Drugs, Chinese Herbal) RN - 0 (Glycolipids) RN - 9009-62-5 (Propolis) SB - IM MH - Animals MH - Biomarkers/blood/metabolism MH - Chromatography, High Pressure Liquid/methods MH - Diabetes Mellitus, Experimental/*drug therapy/*metabolism MH - Drugs, Chinese Herbal/*pharmacology MH - *Ginkgo biloba MH - Glycolipids/blood/*metabolism MH - *Gynostemma MH - Lipid Metabolism/drug effects MH - Male MH - Mass Spectrometry/methods MH - Metabolome/drug effects MH - Metabolomics/methods MH - Phytotherapy/methods MH - Propolis/chemistry/*pharmacology MH - Rats MH - Rats, Wistar OTO - NOTNLM OT - Bio-markers OT - Energy metabolism OT - Metabonomics OT - T2DM OT - TCM EDAT- 2018/09/22 06:00 MHDA- 2018/10/18 06:00 CRDT- 2018/09/22 06:00 PHST- 2018/05/28 00:00 [received] PHST- 2018/08/20 00:00 [revised] PHST- 2018/08/21 00:00 [accepted] PHST- 2018/09/22 06:00 [pubmed] PHST- 2018/10/18 06:00 [medline] PHST- 2018/09/22 06:00 [entrez] AID - S1570-0232(18)30839-0 [pii] AID - 10.1016/j.jchromb.2018.08.023 [doi] PST - ppublish SO - J Chromatogr B Analyt Technol Biomed Life Sci. 2018 Oct 15;1097-1098:128-141. doi: 10.1016/j.jchromb.2018.08.023. Epub 2018 Aug 24.